Browse by Research Team

American Journal of Physiology Endocrinology and Metabolism

Susceptibility to diet-induced obesity at thermoneutral conditions is independent of UCP1.
Dieckmann, S., Strohmeyer, A., Willershäuser, M., Maurer, S.F., Wurst, W., Marschall, S., Hrabě de Angelis, M., Kühn, R., Worthmann, A., Fuh, M.M., Heeren, J., Köhler, N., Pauling, J.K. and Klingenspor, M.
American Journal of Physiology Endocrinology and Metabolism 322 (2): E85-E100. February 2022

Annual Review of Genetics

The functional annotation of mammalian genomes: the challenge of phenotyping.
Brown, S.D.M., Wurst, W., Kühn, R. and Hancock, J.M.
Annual Review of Genetics 43 : 305-333. December 2009

BMC Biology

Genome-wide screening reveals the genetic basis of mammalian embryonic eye development.
Chee, J.M., Lanoue, L., Clary, D., Higgins, K., Bower, L., Flenniken, A., Guo, R., Adams, D.J., Bosch, F., Braun, R.E., Brown, S.D.M., Chin, H.J.G., Dickinson, M.E., Hsu, C.W., Dobbie, M., Gao, X., Galande, S., Grobler, A., Heaney, J.D., Herault, Y., de Angelis, M.H., Mammano, F., Nutter, L.M.J, Parkinson, H., Qin, C., Shiroishi, T., Sedlacek, R., Seong, J.K., Xu, Y., Brooks, B., McKerlie, C., Lloyd, K.C.K., Westerberg, H. and Moshiri, A.
BMC Biology 21 (1): 22. 3 February 2023

BMC Biotechnology

Enhancement of CRISPR-Cas9 induced precise gene editing by targeting Histone H2A-K15 ubiquitination.
Bashir, S., Dang, T., Rossius, J., Wolf, J. and Kuehn, R.
BMC Biotechnology 20 (1): 57. 23 October 2020

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes.
Chu, V.T., Weber, T., Graf, R., Sommermann, T., Petsch, K., Sack, U., Volchkov, P., Rajewsky, K. and Kühn, R.
BMC Biotechnology 16 (1): 4. 16 January 2016

Bio-protocol

Expansion and precise CRISPR-Cas9 gene repair of autologous T memory stem cells from patients with T cell immunodeficiencies.
Li, X., Chu, V.T., Kocks, C. and Rajewsky, K.
Bio-protocol 14 (20): e5085. 20 October 2024

Application of a spacer-nick gene-targeting approach to repair disease-causing mutations with increased safety.
Tran, N.T., Lebedin, M., Danner, E., Kühn, R., Rajewsky, K. and Chu, V.T.
Bio-protocol 13 (8): e4661. 20 April 2023

Biochimica et Biophysica Acta - Molecular Basis of Disease

Elevated glutaric acid levels in Dhtkd1-/Gcdh- double knockout mice challenge our current understanding of lysine metabolism.
Biagosch, C., Ediga, R.D., Hensler, S.V., Faerberboeck, M., Kuehn, R., Wurst, W., Meitinger, T., Kölker, S., Sauer, S. and Prokisch, H.
Biochimica et Biophysica Acta - Molecular Basis of Disease 1863 (9): 2220-2228. September 2017

Blood

Chronic CD30-signaling in B cells results in lymphomagenesis by driving the expansion of plasmablasts and B1 cells.
Sperling, S.A., Fiedler, P., Lechner, M., Pollithy, A., Ehrenberg, S., Schiefer, A.I., Kenner, L., Feuchtinger, A., Kühn, R., Swinerd, G., Schmidt-Supprian, M., Strobl, L.J. and Zimber-Strobl, U.
Blood 133 (24): 2597-2609. 13 June 2019

CD19-independent instruction of murine marginal zone B-cell development by constitutive Notch2 signaling.
Hampel, F., Ehrenberg, S., Hojer, C., Draeseke, A., Marschall-Schröter, G., Kühn, R., Mack, B., Gires, O., Vahl, C.J., Schmidt-Supprian, M., Strobl, L.J. and Zimber-Strobl, U.
Blood 118 (24): 6321-631. 8 December 2011

Cancers

A RAS-independent biomarker panel to reliably predict response to MEK inhibition in colorectal cancer.
Pfohl, U., Loskutov, J., Bashir, S., Kühn, R., Herter, P., Templin, M., Mamlouk, S., Belanov, S., Linnebacher, M., Bürtin, F., Vetter, M., Reinhard, C., Wedeken, L. and Regenbrecht, C.R.A.
Cancers 14 (13): 3252. 1 July 2022

Cell

Mutations in disordered regions can cause disease by creating dileucine motifs.
Meyer, K., Kirchner, M., Uyar, B., Cheng, J.Y., Russo, G., Hernandez-Miranda, L.R., Szymborska, A., Zauber, H., Rudolph, I.M., Willnow, T.E., Akalin, A., Haucke, V., Gerhardt, H., Birchmeier, C., Kühn, R., Krauss, M., Diecke, S., Pascual, J.M. and Selbach, M.
Cell 175 (1): 239-253. 20 September 2018

In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death.
Lam, K.P., Kühn, R. and Rajewsky, K.
Cell 90 (6): 1073-1083. 19 September 1997

Interleukin-10-deficient mice develop chronic enterocolitis.
Kühn, R., Löhler, J., Rennick, D., Rajewsky, K. and Müller, W.
Cell 75 (2): 263-274. 22 October 1993

Cell Reports

Microglia sense neuronal activity via GABA in the early postnatal hippocampus.
Logiacco, F., Xia, P., Georgiev, S.V., Franconi, C., Chang, Y.J., Ugursu, B., Sporbert, A., Kühn, R., Kettenmann, H. and Semtner, M.
Cell Reports 37 (13): 110128. 28 December 2021

Efficient CRISPR/Cas9-mediated gene knockin in mouse hematopoietic stem and progenitor cells.
Tran, N.T., Sommermann, T., Graf, R., Trombke, J., Pempe, J., Petsch, K., Kühn, R., Rajewsky, K. and Chu, V.T.
Cell Reports 28 (13): 3510-3522. 24 September 2019

Cells

Generation of a NES-mScarlet red fluorescent reporter human iPSC line for live cell imaging and flow cytometric analysis and sorting using CRISPR-Cas9-mediated gene editing.
Nouri, P., Zimmer, A., Brüggemann, S., Friedrich, R., Kühn, R. and Prakash, N.
Cells 11 (2): 268. 13 January 2022

Cellular Reprogramming

Human induced pluripotent stem cell-derived pericytes as scalable and editable source to study direct lineage reprogramming into induced neurons.
Menon, R., Petrucci, L., Lohrer, B., Zhang, J., Schulze, M., Schichor, C., Winner, B., Winkler, J., Riemenschneider, M.J., Kühn, R., Falk, S. and Karow, M.
Cellular Reprogramming 25 (5): 212-223. 13 October 2023

Current Opinion in Immunology

Advances in gene targeting methods.
Kühn, R. and Schwenk, F.
Current Opinion in Immunology 9 (2): 183-188. April 1997

Current Protocols in Mouse Biology

Gene editing in one-cell embryos by zinc-finger and TAL nucleases.
Wefers, B., Meyer, M., Hensler, S., Panda, S., Ortiz, O., Wurst, W. and Kühn, R.
Current Protocols in Mouse Biology 2 : 347-364. December 2012

Design and generation of gene-targeting vectors.
Wefers, B., Wurst, W. and Kühn, R.
Current Protocols in Mouse Biology 1 : 199-211. March 2011

Development

Caspase-mediated apoptosis induction in zebrafish cerebellar Purkinje neurons.
Weber, T., Namikawa, K., Winter, B., Müller-Brown, K., Kühn, R., Wurst, W. and Köster, R.W.
Development 143 (22): 4279-4287. 15 November 2016

Drug Discovery Today: Disease Models

Genome wide conditional mouse knockout resources.
Kaloff, C., Anastassiadis, K., Ayadi, A., Baldock, R., Beig, J., Birling, M.C., Bradley, A., Brown, S.D.M., Buerger, A., Bushell, W., Chiani, F., Collins, F.S., Doe, B., Eppig, J.T., Finnell, R.H., Fletcher, C., Flicek, P., Fray, M., Friedel, R.H., Gambadoro, A., Gates, H., Hansen, J., Herault, Y., Hicks, G.G., Hoerlein, A., Hrabe de Angelis, M., Iyer, V., de Jong, P.J., Koscielny, G., Kuehn, R., Liu, P., Lloyd, K.C.K, Lopez, R.G., Marschall, S., Martinez, S., McKerlie, C., Meehan, T., von Melchner, H., Moore, M., Murray, S.A., Nagy, A., Nutter, L.M.J., Pavlovic, G., Pombero, A., Prosser, H., Ramirez-Solis, R., Ringwald, M., Rosen, B., Rosenthal, N., Rossant, J., Ruiz Noppinger, P., Ryder, E., Skarnes, W.C., Schick, J., Schnuetgen, F., Schofield, P., Seisenberger, C., Selloum, M., Smedley, D., Simpson, E.M., Stewart, A.F., Teboul, L., Tocchini Valentini, G.P., Valenzuela, D., West, A.P. and Wurst, W.
Drug Discovery Today: Disease Models 20 : 3-12. 2016

EMBO Reports

Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells.
Lommel, S., Benesch, S., Rottner, K., Franz, T., Wehland, J. and Kühn, R.
EMBO Reports 2 (9): 850-857. September 2001

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Mouse mutagenesis and gene function.
Kühn, R. and Wurst, W.
In: Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. Wiley, New York, U.S.A., 1184-1201. ISBN 978-0-470-84974-3 15 April 2005

European Journal of Immunology

A novel allele for inducible Cre expression in germinal center B cells.
Weber, T., Seagal, J., Winkler, W., Wirtz, T., Chu, V.T. and Rajewsky, K.
European Journal of Immunology 49 (1): 192-194. January 2019

Somatic hypermutation occurs in B cells of terminal deoxynucleotidyl transferase-, CD23-, interleukin-4-, IgD- and CD30-deficient mouse mutants.
Texidó, G., Jacobs, H., Meiering, M., Kühn, R., Roes, J., Müller, W., Gilfillan, S., Fujiwara, H., Kikutani, H., Yoshida, N., Amakawa, R., Benoist, C., Mathis, D., Kishimoto, T., Mak, T.W. and Rajewsky, K.
European Journal of Immunology 26 (8): 1966-1969. August 1996

Development and proliferation of lymphocytes in mice deficient for both interleukins-2 and -4.
Sadlack, B., Kühn, R., Schorle, H., Rajewsky, K., Müller, W. and Horak, I.
European Journal of Immunology 24 (1): 281-284. January 1994

Interleukin-4 transgenic mice of resistant background are susceptible to Leishmania major infection.
Leal, L.M.C.C., Moss, D.W., Kuhn, R., Müller, W. and Liew, F.Y.
European Journal of Immunology 23 (2): 566-569. February 1993

Major histocompatibility complex class II hyperexpression on B cells in interleukin 4-transgenic mice does not lead to B cell proliferation and hypergammaglobulinemia.
Müller, W., Kühn, R. and Rajewsky, K.
European Journal of Immunology 21 (4): 921-925. April 1991

European Journal of Neuroscience

Connexin43 is not expressed in principal cells of mouse cortex and hippocampus.
Theis, M., Soehl, G., Speidel, D., Kühn, R. and Willecke, K.
European Journal of Neuroscience 18 (2): 267-274. July 2003

Experimental Neurology

Pink1-deficiency in mice impairs gait, olfaction and serotonergic innervation of the olfactory bulb.
Glasl, L., Kloos, K., Giesert, F., Roethig, A., Di Benedetto, B., Kühn, R., Zhang, J., Hafen, U., Zerle, J., Hofmann, A., de Angelis, M.H., Winklhofer, K.F., Hölter, S.M., Vogt Weisenhorn, D.M. and Wurst, W.
Experimental Neurology 235 (1): 214-227. May 2012

Experimental Parasitology

Plasmodium chabaudi chabaudi: differential susceptibility of gene-targeted mice deficient in IL-10 to an erythrocytic-stage infection.
Linke, A, Kühn, R., Müller, W, Honarvar, N, Li, C and Langhorne, J
Experimental Parasitology 84 (2): 253-263. November 1996

FEBS Open Bio

Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos.
Brandl, C., Ortiz, O., Röttig, B., Wefers, B., Wurst, W. and Kühn, R.
FEBS Open Bio 5 : 26-35. 2015

Frontiers in Behavioral Neuroscience

Conditional reduction of adult born doublecortin-positive neurons reversibly impairs selective behaviors.
Garrett, L., Zhang, J., Zimprich, A., Niedermeier, K.M., Fuchs, H., Gailus-Durner, V., Hrabe de Angelis, M., Vogt Weisenhorn, D., Wurst, W. and Hoelter, S.M.
Frontiers in Behavioral Neuroscience 9 : 302. 12 November 2015

Frontiers in Genetics

Efficient and precise CRISPR/Cas9-mediated MECP2 modifications in human-induced pluripotent stem cells.
Le, T.T.H., Tran, N.T., Dao, T.M.L., Nguyen, D.D., Do, H.D., Ha, T.L., Kühn, R., Nguyen, T.L., Rajewsky, K. and Chu, V.T.
Frontiers in Genetics 10 : 625. July 2019

Enhancement of precise gene editing by the association of Cas9 with homologous recombination factors.
Tran, N.T., Bashir, S., Li, X., Rossius, J., Chu, V.T., Rajewsky, K. and Kühn, R.
Frontiers in Genetics 10 : 365. 30 April 2019

Frontiers in Immunology

A CRISPR/Cas9-mediated screen identifies determinants of early plasma cell differentiation.
Xiong, E., Popp, O., Salomon, C., Mertins, P., Kocks, C., Rajewsky, K. and Chu, V.T.
Frontiers in Immunology 13 : 1083119. 5 January 2023

B-cell-specific Myd88 L252P expression causes a premalignant gammopathy resembling IgM MGUS.
Schmidt, K., Sack, U., Graf, R., Winkler, W., Popp, O., Mertins, P., Sommermann, T., Kocks, C. and Rajewsky, K.
Frontiers in Immunology 11 : 602868. 1 December 2020

Frontiers in Neuroscience

Novel human neurodevelopmental and neurodegenerative disease associated with IRF2BPL gene variants-mechanisms and therapeutic avenues.
Bauersachs, D., Bomholtz, L., Del Ray Mateos, S., Kühn, R. and Lisowski, P.
Frontiers in Neuroscience 18 : 1426177. 6 June 2024

Gene and Genome Editing

The methylation inhibitor 3DZNep promotes HDR pathway choice during CRISPR-Cas9 genome editing.
Bischoff, N., Wimberger, S., Kuehn, R., Laugesen, A., Turan, V., Larsen, B.D., Sørensen, C.S., Helin, K., Bennett, E.P., Maresca, M. and Brakebusch, C.
Gene and Genome Editing 5 : 100023. June 2023

Genes

A mouse model of X-linked chronic granulomatous disease for the development of CRISPR/Cas9 gene therapy.
Sevim-Wunderlich, S., Dang, T., Rossius, J., Schnütgen, F. and Kuehn, R.
Genes 15 (6): 706. June 2024

Genes & Development

Oscillations of MyoD and Hes1 proteins regulate the maintenance of activated muscle stem cells.
Lahmann, I., Bröhl, D., Zyrianova, T., Isomura, A., Czajkowski, M.T., Kapoor, V., Griger, J., Ruffault, P.L., Mademtzoglou, D., Zammit, P.S., Wunderlich, T., Spuler, S., Kühn, R., Preibisch, S., Wolf, J., Kageyama, R. and Birchmeier, C.
Genes & Development 33 (9-10): 524-535. 1 May 2019

Genes Brain and Behavior

Genetic mouse models for behavioral analysis through transgenic RNAi technology.
Delic, S., Streif, S., Deussing, J.M., Weber, P., Ueffing, M., Hoelter, S.M., Wurst, W. and Kühn, R.
Genes Brain and Behavior 7 (7): 821-830. October 2008

Genesis

Reversible and tissue-specific activation of MAP kinase signaling by tamoxifen in Braf(V637)ER(T2) mice.
Ortiz, O., Wurst, W. and Kühn, R.
Genesis 51 (6): 448-455. June 2013

Novel caspase-suicide proteins for tamoxifen-inducible apoptosis.
Chu, Y., Senghaas, N., Koester, R.W., Wurst, W. and Kühn, R.
Genesis 46 (10): 530-536. October 2008

Sall4 isoforms act during proximal-distal and anterior-posterior axis formation in the mouse embryo.
Uez, N., Lickert, H., Kohlhase, J., de Angelis, M.H., Kühn, R., Wurst, W. and Floss, T.
Genesis 46 (9): 463-477. September 2008

Simultaneous Cre-mediated conditional knockdown of two genes in mice.
Steuber-Buchberger, P., Wurst, W. and Kühn, R.
Genesis 46 (3): 144-151. March 2008

Genetics

Highly efficient targeted mutagenesis in mice using TALENs.
Panda, S.K., Wefers, B., Ortiz, O., Floss, T., Schmid, B., Haass, C., Wurst, W. and Kühn, R.
Genetics 195 (3): 703-713. November 2013

Genome Biology

Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9.
Kühn, R. and Chu, V.T.
Genome Biology 16 (1): 244. 10 November 2015

Genome Research

Phenotypic annotation of the mouse X chromosome.
Cox, B.J., Vollmer, M., Tamplin, O., Lu, M., Biechele, S., Gertsenstein, M., van Campenhout, C., Floss, T., Kühn, R., Wurst, W., Lickert, H. and Rossant, J.
Genome Research 20 (8): 1154-1164. August 2010

Glia

Inducible gene deletion in astroglia and radial glia--a valuable tool for functional and lineage analysis.
Mori, T., Tanaka, K., Buffo, A., Wurst, W., Kühn, R. and Goetz, M.
Glia 54 (1): 21-34. July 2006

Handbook of Experimental Pharmacology

RNA interference in mice.
Kühn, R., Streif, S. and Wurst, W.
Handbook of Experimental Pharmacology 178 (1): 149-176. 2007

Handbuch der Psychopharmakotherapie

Genetisch veraenderte Tiere.
Kühn, R. and Wurst, W.
In: Handbuch der Psychopharmakotherapie. Springer, 149-167. ISBN 978-3-642-19843-4 2012

Human Molecular Genetics

BACE knockout mice are healthy despite lacking the primary beta-secretase activity in brain: implications for Alzheimer's disease therapeutics.
Roberds, S.L., Anderson, J., Basi, G., Bienkowski, M.J., Branstetter, D.G., Chen, K.S., Freedman, S.B., Frigon, N.L., Games, D., Hu, K., Johnson-Wood, K., Kappenman, K.E., Kawabe, T.T., Kola, I., Kuehn, R., Lee, M., Liu, W., Motter, R., Nichols, N.F., Power, M., Robertson, D.W., Schenk, D., Schoor, M., Shopp, GM., Shuck, M.E., Sinha, S., Svensson, K.A., Tatsuno, G., Tintrup, H., Wijsman, J., Wright, S. and McConlogue, L.
Human Molecular Genetics 10 (12): 1317-1324. 1 June 2001

Immunological Reviews

Common cytokine receptor gamma chain (gamma c)-dependent cytokines: understanding in vivo functions by gene targeting.
Di Santo, J.P., Kühn, R. and Mueller, W.
Immunological Reviews 148 (1): 19-34. December 1995

JCI Insight

Base editing repairs an SGCA mutation in human primary muscle stem cells.
Escobar, H., Krause, A., Keiper, S., Kieshauer, J., Müthel, S., García de Paredes, M., Metzler, E., Kühn, R., Heyd, F. and Spuler, S.
JCI Insight 6 (10): e145994. 24 May 2021

Journal of Biotechnology

Fusion of SpCas9 to E.coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.
Lin, L., Petersen, T.S., Jensen, K.T., Bolund, L., Kühn, R. and Luo, Y.
Journal of Biotechnology 247 : 42-49. 10 April 2017

Journal of Clinical Investigation

Enterocolitis and colon cancer in interleukin-10-deficient mice are associated with aberrant cytokine production and CD4(+) TH1-like responses.
Berg, D.J., Davidson, N., Kühn, R., Müller, W., Menon, S., Holland, G., Thompson-Snipes, L., Leach, M.W. and Rennick, D.
Journal of Clinical Investigation 98 (4): 1010-1020. 15 August 1996

Conditional gene targeting.
Rajewsky, K., Gu, H., Kühn, R., Betz, U.A., Müller, W., Roes, J. and Schwenk, F.
Journal of Clinical Investigation 98 (3): 600-603. 1 August 1996

Interleukin-10 is a central regulator of the response to LPS in murine models of endotoxic shock and the Shwartzman reaction but not endotoxin tolerance.
Berg, D.J., Kühn, R., Rajewsky, K., Müller, W., Menon, S., Davidson, N., Grünig, G. and Rennick, D.
Journal of Clinical Investigation 96 (5): 2339-2347. November 1995

Journal of Comparative Neurology

Differential mRNA distribution of components of the ERK/MAPK signalling cascade in the adult mouse brain.
Di Benedetto, B., Hitz, C., Hoelter, S.M., Kuehn, R., Vogt Weisenhorn, D.M. and Wurst, W.
Journal of Comparative Neurology 500 (3): 542-556. 20 January 2007

Journal of Experimental Medicine

Interleukin (IL)-4-independent immunoglobulin class switch to immunoglobulin (Ig)E in the mouse.
Morawetz, R.A., Gabriele, L., Rizzo, L.V., Noben-Trauth, N., Kühn, R., Rajewsky, K., Müller, W., Doherty, T.M., Finkelman, F., Coffman, R.L. and Morse, H.C.
Journal of Experimental Medicine 184 (5): 1651-1661. 1 November 1996

T helper cell 1-type CD4+ T cells, but not B cells, mediate colitis in interleukin 10-deficient mice.
Davidson, N.J., Leach, M.W., Fort, M.M., Thompson-Snipes, L., Kühn, R., Müller, W., Berg, D.J. and Rennick, D.M.
Journal of Experimental Medicine 184 (1): 241-251. 1 July 1996

Leishmania promastigotes selectively inhibit interleukin 12 induction in bone marrow-derived macrophages from susceptible and resistant mice.
Carrera, L., Gazzinelli, R.T., Badolato, R., Hieny, S., Mueller, W., Kühn, R. and Sacks, D.L.
Journal of Experimental Medicine 183 (2): 515-526. 1 February 1996

Interleukin 10 but not interleukin 4 is a natural suppressant of cutaneous inflammatory responses.
Berg, D.J., Leach, M.W., Kühn, R., Rajewsky, K., Müller, W., Davidson, N.J. and Rennick, D.
Journal of Experimental Medicine 182 (1): 99-108. 1 July 1995

Induction of interleukin 4 (IL-4) expression in T helper (Th) cells is not dependent on IL-4 from non-Th cells.
Schmitz, J., Thiel, A., Kühn, R., Rajewsky, K., Müller, W., Assenmacher, M. and Radbruch, A.
Journal of Experimental Medicine 179 (4): 1349-53. 1 April 1994

Journal of Immunological Methods

Generation of Cre recombinase-specific monoclonal antibodies, able to characterize the pattern of Cre expression in cre-transgenic mouse strains.
Schwenk, F., Sauer, B., Kukoč, N., Hoess, R., Müller, W., Kocks, C., Kühn, R. and Rajewsky, K.
Journal of Immunological Methods 207 (2): 203-212. 24 September 1997

Journal of Immunology

IL-10 regulates liver pathology in acute murine Schistosomiasis mansoni but is not required for immune down-modulation of chronic disease.
Wynn, T.A., Cheever, A.W., Williams, M.E., Hieny, S., Caspar, P., Kühn, R., Müller, W. and Sher, A.
Journal of Immunology 160 (9): 4473-4480. 1 May 1998

In the absence of endogenous IL-10, mice acutely infected with Toxoplasma gondii succumb to a lethal immune response dependent on CD4+ T cells and accompanied by overproduction of IL-12, IFN-gamma and TNF-alpha.
Gazzinelli, R.T., Wysocka, M., Hieny, S., Scharton-Kersten, T., Cheever, A., Kühn, R., Mueller, W., Trinchieri, G. and Sher, A.
Journal of Immunology 157 (2): 798-805. 15 July 1996

IL-9 production of naive CD4+ T cells depends on IL-2, is synergistically enhanced by a combination of TGF-beta and IL-4, and is inhibited by IFN-gamma.
Schmitt, E., Germann, T., Goedert, S., Hoehn, P., Huels, C., Koelsch, S., Kuehn, R., Mueller, W., Palm, N. and Ruede, E.
Journal of Immunology 153 (9): 3989-3996. 1 November 1994

T helper cell membranes promote IL-4-independent expression of germ-line Cγ1 transcripts in B cells.
Schultz, C.L., Rothman, P., Kühn, R., Kehry, M., Müller, W., Rajewsky, K., Alt, F. and Coffman, R.L.
Journal of Immunology 149 (1): 60-64. 1 July 1992

Signal requirements for growth and differentiation of activated murine B lymphocytes.
Müller, W., Kühn, R., Goldmann, W., Tesch, H., Smith, F.I., Radbruch, A. and Rajewsky, K.
Journal of Immunology 135 (2): 1213-1219. August 1985

Journal of Investigative Dermatology

Impaired immunosuppressive response to ultraviolet radiation in interleukin-10-deficient mice.
Beissert, S., Hosoi, J., Kühn, R., Rajewsky, K., Müller, W. and Granstein, R.D.
Journal of Investigative Dermatology 107 (4): 553-557. October 1996

Journal of Neuroscience

Regulation of the natriuretic peptide receptor 2 (Npr2) by phosphorylation of juxtamembrane serine and threonine residues is essential for bifurcation of sensory axons.
Schmidt, H., Dickey, D.M., Dumoulin, A., Octave, M., Robinson, J.W., Kühn, R., Feil, R., Potter, L.R. and Rathjen, F.G.
Journal of Neuroscience 38 (45): 9768-9780. 7 November 2018

DNA hypomethylation perturbs the function and survival of CNS neurons in postnatal animals.
Fan, G., Beard, C., Chen, R.Z., Csankovszki, G., Sun, Y., Siniaia, M., Biniszkiewicz, D., Bates, B., Lee, P.P., Kühn, R., Trumpp, A., Poon, C., Wilson, C.B. and Jaenisch, R.
Journal of Neuroscience 21 (3): 788-797. 1 February 2001

Journal of Neuroscience Research

Forebrain-specific knockout of B-raf kinase leads to deficits in hippocampal long-term potentiation, learning, and memory.
Chen, A.P., Ohno, M., Giese, K.P., Kühn, R., Chen, R.L. and Silva, A.J.
Journal of Neuroscience Research 83 (1): 28-38. January 2006

Journal of Virology

Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4.
Bachmann, M.F., Schorle, H., Kuehn, R., Mueller, W., Hengartner, H., Zinkernagel, R.M. and Horak, I.
Journal of Virology 69 (8): 4842-4846. August 1995

Laboratory Animals

Genome engineering in rodents - status quo and perspectives.
Kühn, R.
Laboratory Animals 56 (1): 83-87. 1 February 2022

Leukemia

In vivo CRISPR/Cas9-mediated screen reveals a critical function of TFDP1 and E2F4 transcription factors in hematopoiesis.
Tran, N.T., Graf, R., Acevedo-Ochoa, E., Trombke, J., Weber, T., Sommermann, T., Salomon, C., Kühn, R., Rajewsky, K. and Chu, V.T.
Leukemia 38 (9): 2003-2015. September 2024

Life Science Alliance

Branch point strength controls species-specific CAMK2B alternative splicing and regulates LTP.
Franz, A., Weber, A.I., Preußner, M., Dimos, N., Stumpf, A., Ji, Y., Moreno-Velasquez, L., Voigt, A., Schulz, F., Neumann, A., Kuropka, B., Kuehn, R., Urlaub, H., Schmitz, D., Wahl, M.C. and Heyd, F.
Life Science Alliance 6 (3): e202201826. March 2023

Mammalian Genome

A deletion containing a CTCF-element in intron 8 of the Bbs7 gene is partially responsible for juvenile obesity in the Berlin Fat Mouse.
Krause, F., Mohebian, K., Delpero, M., Hesse, D., Kuehn, R., Arends, D. and Brockmann, G.A.
Mammalian Genome 33 (3): 465–470. September 2022

Control of gene editing by manipulation of DNA repair mechanisms.
Danner, E., Bashir, S., Yumlu, S., Wurst, W., Wefers, B. and Kühn, R.
Mammalian Genome 28 (7-8): 262-274. August 2017

Methods

Gene editing and clonal isolation of human induced pluripotent stem cells using CRISPR/Cas9.
Yumlu, S., Stumm, J., Bashir, S., Dreyer, A.K., Lisowski, P., Danner, E. and Kühn, R.
Methods 121-122 : 29-44. 15 May 2017

Gene editing in mouse zygotes using the CRISPR/Cas9 system.
Wefers, B., Bashir, S., Rossius, J. and Kühn, R.
Methods 121-122 : 55-67. 15 May 2017

Editing and investigating genomes with TALE and CRISPR/Cas systems: Genome engineering across species using TALENs.
Kuehn, R. and Wefers, B.
Methods 69 (1): 1. 15 August 2014

Generation of targeted mouse mutants by embryo microinjection of TALENs.
Wefers, B., Ortiz, O., Wurst, W. and Kühn, R.
Methods 69 (1): 94-101. 15 August 2014

Methods in Enzymology

Gene knockdown in the mouse through RNAi.
Kleinhammer, A., Wurst, W. and Kühn, R.
Methods in Enzymology 477 : 387-414. 2010

Methods in Molecular Biology

Gene editing in mouse zygotes using the CRISPR/Cas9 system.
Wefers, B., Wurst, W. and Kühn, R.
Methods in Molecular Biology 2631 : 207-230. 2023

Efficient gene editing of human induced pluripotent stem cells using CRISPR/Cas9.
Yumlu, S., Bashir, S., Stumm, J. and Kühn, R.
Methods in Molecular Biology 1961 : 137-151. 2019

Genome editing in mice using TALE nucleases.
Wefers, B., Brandl, C., Ortiz, O., Wurst, W. and Kühn, R.
Methods in Molecular Biology 1338 : 229-243. 2016

Target validation in mice by constitutive and conditional RNAi.
Kleinhammer, A., Wurst, W. and Kühn, R.
Methods in Molecular Biology 986 : 307-323. 23 January 2013

Generating conditional knockout mice.
Friedel, R.H., Wurst, W., Wefers, B. and Kuehn, R.
Methods in Molecular Biology 693 : 205-231. 2011

Gene knockout protocols.
Wurst, W. and Kuehn, R., eds.
Methods in Molecular Biology, 530 . Springer / Humana Press. ISBN 978-1-934115-26-8 2009

Generation of shRNA transgenic mice.
Hitz, C., Steuber-Buchberger, P., Delic, S., Wurst, W. and Kuehn, R.
Methods in Molecular Biology 530 : 101-129. 2009

Overview on mouse mutagenesis.
Kühn, R. and Wurst, W.
Methods in Molecular Biology 530 : 1-12. 2009

Conditional knockout mice.
Kühn, R. and Schwenk, F.
Methods in Molecular Biology 209 : 159-185. 2002

Cre/loxP recombination system and gene targeting.
Kühn, R. and Torres, R.M.
Methods in Molecular Biology 180 (5): 175-204. 2002

Molecular Biotechnology

Simple derivation of transgene-free iPS cells by a dual recombinase approach.
Pertek, A., Meier, F., Irmler, M., Beckers, J., Skylaki, S., Endele, M., Wurst, W., Prakash, N. and Kühn, R.
Molecular Biotechnology 56 (8): 697-713. August 2014

Local knockdown of ERK2 in the adult mouse brain via adeno-associated virus-mediated RNA interference.
Di Benedetto, B., Wefers, B., Wurst, W. and Kühn, R.
Molecular Biotechnology 41 (3): 263-269. March 2009

Molecular Endocrinology

Conditional deletion of brain-derived neurotrophic factor in the postnatal brain leads to obesity and hyperactivity.
Rios, M., Fan, G., Fekete, C., Kelly, J., Bates, B., Kuehn, R., Lechan, R.M. and Jaenisch, R.
Molecular Endocrinology 15 (10): 1748-1757. October 2001

Molecular Therapy

CRISPR/Cas9-mediated ELANE mutation correction in hematopoietic stem and progenitor cells to treat severe congenital neutropenia.
Tran, N.T., Graf, R., Wulf-Goldenberg, A., Stecklum, M., Strauß, G., Kühn, R., Kocks, C., Rajewsky, K. and Chu, V.T.
Molecular Therapy 28 (12): 2621-2634. 2 December 2020

Molecular and Cellular Biology

Hybrid embryonic stem cell-derived tetraploid mice show apparently normal morphological, physiological, and neurological characteristics.
Schwenk, F., Zevnik, B., Bruening, J., Roehl, M., Willuweit, A., Rode, A., Hennek, T., Kauselmann, G., Jaenisch, R. and Kühn, R.
Molecular and Cellular Biology 23 (11): 3982-3989. June 2003

Nature

Csk controls antigen receptor-mediated development and selection of T-lineage cells.
Schmedt, C., Saijo, K., Niidome, T., Kühn, R., Aizawa, S. and Tarakhovsky, A.
Nature 394 (6696): 901-904. 27 August 1998

Requirement of mammalian DNA polymerase-beta in base-excision repair.
Sobol, R.W., Horton, J.K., Kühn, R., Gu, H., Singhal, R.K., Prasad, R., Rajewsky, K. and Wilson, S.H.
Nature 379 (6561): 183-186. 11 January 1996

A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin μ chain gene.
Kitamura, D., Roes, J., Kühn, R. and Rajewsky, K.
Nature 350 (6317): 423-426. 4 April 1991

Nature Biomedical Engineering

Enhanced precision and efficiency.
Bashir, S. and Kühn, R.
Nature Biomedical Engineering 1 (11): 856-857. November 2017

Nature Biotechnology

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Chu, V.T., Weber, T., Wefers, B., Wurst, W., Sander, S., Rajewsky, K. and Kühn, R.
Nature Biotechnology 33 (5): 543-548. May 2015

Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation.
Eggan, K., Rode, A., Jentsch, I., Samuel, C., Hennek, T., Tintrup, H., Zevnik, B., Erwin, J., Loring, J., Jackson-Grusby, L., Speicher, M.R., Kuehn, R. and Jaenisch, R.
Nature Biotechnology 20 (5): 455-459. May 2002

Nature Communications

Mutant huntingtin impairs neurodevelopment in human brain organoids through CHCHD2-mediated neurometabolic failure.
Lisowski, P., Lickfett, S., Rybak-Wolf, A., Menacho, C., Le, S., Pentimalli, T.M., Notopoulou, S., Dykstra, W., Oehler, D., López-Calcerrada, S., Mlody, B., Otto, M., Wu, H., Richter, Y., Roth, P., Anand, R., Kulka, L.A.M., Meierhofer, D., Glazar, P., Legnini, I., Telugu, N.S., Hahn, T., Neuendorf, N., Miller, D.C., Böddrich, A., Polzin, A., Mayatepek, E., Diecke, S., Olzscha, H., Kirstein, J., Ugalde, C., Petrakis, S., Cambridge, S., Rajewsky, N., Kühn, R., Wanker, E.E., Priller, J., Metzger, J.J. and Prigione, A.
Nature Communications 15 (1): 7027. 22 August 2024

Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth.
Dzamukova, M., Brunner, T.M., Miotla-Zarebska, J., Heinrich, F., Brylka, L., Mashreghi, M.F., Kusumbe, A., Kühn, R., Schinke, T., Vincent, T.L.. and Löhning, M.
Nature Communications 13 (1): 3059. 1 June 2022

Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome.
Inak, G., Rybak-Wolf, A., Lisowski, P., Pentimalli, T.M., Jüttner, R., Glažar, P., Uppal, K., Bottani, E., Brunetti, D., Secker, C., Zink, A., Meierhofer, D., Henke, M.T., Dey, M., Ciptasari, U., Mlody, B., Hahn, T., Berruezo-Llacuna, M., Karaiskos, N., Di Virgilio, M., Mayr, J.A., Wortmann, S.B., Priller, J., Gotthardt, M., Jones, D.P., Mayatepek, E., Stenzel, W., Diecke, S., Kühn, R., Wanker, E.E., Rajewsky, N., Schuelke, M. and Prigione, A.
Nature Communications 12 (1): 1929. 26 March 2021

Human and mouse essentiality screens as a resource for disease gene discovery.
Cacheiro, P., Muñoz-Fuentes, V., Murray, S.A., Dickinson, M.E., Bucan, M., Nutter, L.M.J., Peterson, K.A., Haselimashhadi, H., Flenniken, A.M., Morgan, H., Westerberg, H., Konopka, T., Hsu, C.W., Christiansen, A., Lanza, D.G., Beaudet, A.L., Heaney, J.D., Fuchs, H., Gailus-Durner, V., Sorg, T., Prochazka, J., Novosadova, V., Lelliott, C.J., Wardle-Jones, H., Wells, S., Teboul, L., Cater, H., Stewart, M., Hough, T., Wurst, W., Sedlacek, R., Adams, D.J., Seavitt, J.R., Tocchini-Valentini, G., Mammano, F., Braun, R.E., McKerlie, C., Herault, Y., de Angelis, M.H., Mallon, A.M., Lloyd, K.C.K., Brown, S.D.M., Parkinson, H., Meehan, T.F. and Smedley, D.
Nature Communications 11 (1): 655. 31 January 2020

Identification of genetic elements in metabolism by high-throughput mouse phenotyping.
Rozman, J., Rathkolb, B., Oestereicher, M.A., Schütt, C., Ravindranath, A.C., Leuchtenberger, S., Sharma, S., Kistler, M., Willershäuser, M., Brommage, R., Meehan, T.F., Mason, J., Haselimashhadi, H., Hough, T., Mallon, A.M., Wells, S., Santos, L., Lelliott, C.J., White, J.K., Sorg, T., Champy, M.F., Bower, L.R., Reynolds, C. L., Flenniken, A.M., Murray, St.A., Nutter, L.M.J., Svenson, K.L., West, D., Tocchini-Valentini, G.P., Beaudet, A.L., Bosch, F., Braun, R.B., Dobbie, M.S., Gao, X., Herault, Y., Moshiri, A., Moore, B.A., Kent Lloyd, K.C., McKerlie, C., Masuya, H., Tanaka, N., Flicek, P., Parkinson, H.E., Sedlacek, R., Seong, J.K., Wang, C.K.L., Moore, M., Brown, S.D., Tschöp, M.H., Wurst, W., Klingenspor, M., Wolf, E., Beckers, J., Machicao, F., Peter, A., Staiger, H., Häring, H.U., Grallert, H., Campillos, M., Maier, H., Fuchs, H., Gailus-Durner, V., Werner, T. and Hrabe de Angelis, M.
Nature Communications 9 (1): 288. 18 January 2018

A large scale hearing loss screen reveals an extensive unexplored genetic landscape for auditory dysfunction.
Bowl, M.R., Simon, M.M., Ingham, N.J., Greenaway, S., Santos, L., Cater, H., Taylor, S., Mason, J., Kurbatova, N., Pearson, S., Bower, L.R., Clary, D.A., Meziane, H., Reilly, P., Minowa, O., Kelsey, L., Tocchini-Valentini, G.P., Gao, X., Bradley, A., Skarnes, W.C., Moore, M., Beaudet, A.L., Justice, M.J., Seavitt, J., Dickinson, M.E., Wurst, W., Hrabe de Angelis, M., Herault, Y., Wakana, S., Nutter, L.M.J., Flenniken, A.M., McKerlie, C., Murray, S.A., Svenson, K.L., Braun, R.E., West, D.B., Lloyd, K.C.K., Adams, D.J., White, J., Karp, N., Flicek, P., Smedley, D., Meehan, T.F., Parkinson, H.E., Teboul, L.M., Wells, S., Steel, K.P., Mallon, A.M. and Brown, S.D.M.
Nature Communications 8 (1): 886. 12 October 2017

Nature Genetics

In vivo dissection of a clustered-CTCF domain boundary reveals developmental principles of regulatory insulation.
Anania, C., Acemel, R.D., Jedamzick, J., Bolondi, A., Cova, G., Brieske, N., Kühn, R., Wittler, L., Real, F.M. and Lupiáñez, D.G.
Nature Genetics 54 (7): 1026-1036. July 2022

A resource of targeted mutant mouse lines for 5,061 genes.
Birling, M.C., Yoshiki, A., Adams, D.J., Ayabe, S., Beaudet, A.L., Bottomley, J., Bradley, A., Brown, S.D.M., Bürger, A., Bushell, W., Chiani, F., Chin, H.J.G., Christou, S., Codner, G.F., DeMayo, F.J., Dickinson, M.E., Doe, B., Donahue, L.R., Fray, M.D., Gambadoro, A., Gao, X., Gertsenstein, M., Gomez-Segura, A., Goodwin, L.O., Heaney, J.D., Hérault, Y., de Angelis, M.H., Jiang, S.T., Justice, M.J., Kasparek, P., King, R.E., Kühn, R., Lee, H., Lee, Y.J., Liu, Z., Lloyd, K.C.K., Lorenzo, I., Mallon, A.M., McKerlie, C., Meehan, T.F., Fuentes, V.M., Newman, S., Nutter, L.M.J., Oh, G.T., Pavlovic, G., Ramirez-Solis, R., Rosen, B., Ryder, E.J., Santos, L.A., Schick, J., Seavitt, J.R., Sedlacek, R., Seisenberger, C., Seong, J.K., Skarnes, W.C., Sorg, T., Steel, K.P., Tamura, M., Tocchini-Valentini, G.P., Wang, C.K.L., Wardle-Jones, H., Wattenhofer-Donzé, M., Wells, S., Wiles, M.V., Willis, B.J., Wood, J.A., Wurst, W., Xu, Y., Teboul, L. and Murray, S.A.
Nature Genetics 53 (4): 416-419. April 2021

Nature Immunology

A type 1 immunity-restricted promoter of the IL-33 receptor gene directs antiviral T-cell responses.
Brunner, T.M., Serve, S., Marx, A.F., Fadejeva, J., Saikali, P., Dzamukova, M., Durán-Hernández, N., Kommer, C., Heinrich, F., Durek, P., Heinz, G.A., Höfer, T., Mashreghi, M.F., Kühn, R., Pinschewer, D.D. and Löhning, M.
Nature Immunology 25 (2): 256-267. February 2024

IFNγ binding to extracellular matrix prevents fatal systemic toxicity.
Kemna, J., Gout, E., Daniau, L., Lao, J., Weißert, K., Ammann, S., Kühn, R., Richter, M., Molenda, C., Sporbert, A., Zocholl, D., Klopfleisch, R., Schütz, A., Lortat-Jacob, H., Aichele, P., Kammertoens, T. and Blankenstein, T.
Nature Immunology 24 (3): 414-422. March 2023

Nature Neuroscience

Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity.
Fecher, C., Trovò, L., Müller, S.A., Snaidero, N., Wettmarshausen, J., Heink, S., Ortiz, O., Wagner, I., Kühn, R., Hartmann, J., Karl, R.M., Konnerth, A., Korn, T., Wurst, W., Merkler, D., Lichtenthaler, S.F., Perocchi, F. and Misgeld, T.
Nature Neuroscience 22 (10): 1731-1742. October 2019

Limbic corticotropin-releasing hormone receptor 1 mediates anxiety-related behavior and hormonal adaptation to stress.
Mueller, M.B., Zimmermann, S., Sillaber, I., Hagemeyer, T.P., Deussing, J.M., Timpl, P., Kormann, M.S.D., Droste, S.K., Kühn, R., Reul, J.M.H.M., Holsboer, F. and Wurst, W.
Nature Neuroscience 6 (10): 1100-1107. October 2003

Nature Protocols

Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA.
Wefers, B., Panda, S.K., Ortiz, O., Brandl, C., Hensler, S., Hansen, J., Wurst, W. and Kühn, R.
Nature Protocols 8 (12): 2355-2379. December 2013

NeuroMethods

Genetic models of Parkinson's disease.
Kühn, R., Vogt-Weisenhorn, D. and Wurst, W.
NeuroMethods 61 : 243-265. 2012

Neurochemical Research

Neuron-specific ablation of PDGF-B is compatible with normal central nervous system development and astroglial response to injury.
Enge, M., Wilhelmsson, U., Abramsson, A., Stakeberg, J., Kühn, R., Betsholtz, C. and Pekny, M.
Neurochemical Research 28 (2): 271-279. February 2003

Neuron

Prox2 and Runx3 vagal sensory neurons regulate esophageal motility.
Lowenstein, E.D., Ruffault, P.L., Misios, A., Osman, K.L., Li, H., Greenberg, R.S., Thompson, R., Song, K., Dietrich, S., Li, X., Vladimirov, N., Woehler, A., Brunet, J.F., Zampieri, N., Kühn, R., Liberles, S.D., Jia, S., Lewin, G.R., Rajewsky, N., Lever, T.E. and Birchmeier, C.
Neuron 111 (14): 2184-2200.e7. 19 July 2023

Essential role for TrkB receptors in hippocampus-mediated learning.
Minichiello, L., Korte, M., Wolfer, D., Kühn, R., Unsicker, K., Cestari, V., Rossi-Arnaud, C., Lipp, H.P., Bonhoeffer, T. and Klein, R.
Neuron 24 (2): 401-414. October 1999

Neuropharmacology

N-desalkylquetiapine activates ERK1/2 to induce GDNF release in C6 glioma cells: a putative cellular mechanism for quetiapine as antidepressant.
Di Benedetto, B., Kuehn, R., Nothdurfter, C., Rein, T., Wurst, W. and Rupprecht, R.
Neuropharmacology 62 (1): 209-216. January 2012

Nucleic Acids Research

Development of an intein-mediated split-Cas9 system for gene therapy.
Truong, D.J.J., Kühner, K., Kühn, R., Werfel, S., Engelhardt, S., Wurst, W. and Ortiz, O.
Nucleic Acids Research 43 (13): 6450-6458. 27 July 2015

Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference.
Hitz, C., Wurst, W. and Kühn, R.
Nucleic Acids Research 35 (12): e90. June 2007

Development of a species-specific RNA polymerase I-based shRNA expression vector.
Brenz Verca, M.S., Weber, P., Mayer, C., Graf, C., Refojo, D., Kühn, R., Grummt, I. and Lutz, B.
Nucleic Acids Research 35 (2): e10. January 2007

Single copy shRNA configuration for ubiquitous gene knockdown in mice.
Seibler, J., Küter-Luks, B., Kern, H., Streu, S., Plum, L., Mauer, J., Kühn, R., Bruening, J.C. and Schwenk, F.
Nucleic Acids Research 33 (7): e67. 14 April 2005

Rapid generation of inducible mouse mutants.
Seibler, J., Zevnik, B., Küter-Luks, B., Andreas, S., Kern, H., Hennek, T., Rode, A., Heimann, C., Faust, N., Kauselmann, G., Schoor, M., Jaenisch, R., Rajewsky, K., Kühn, R. and Schwenk, F.
Nucleic Acids Research 31 (4): e12. 15 February 2003

Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells.
Andreas, S., Schwenk, F., Kueter-Luks, B., Faust, N. and Kühn, R.
Nucleic Acids Research 30 (11): 2299-2306. 1 June 2002

Temporally and spatially regulated somatic mutagenesis in mice.
Schwenk, F., Kühn, R., Angrand, P.O., Rajewsky, K. and Stewart, A.F.
Nucleic Acids Research 26 (6): 1427-1432. 15 March 1998

Open Biology

A homology independent sequence replacement strategy in human cells using a CRISPR nuclease.
Danner, E., Lebedin, M., de la Rosa, K. and Kühn, R.
Open Biology 11 (1): 200283. January 2021

PLoS Biology

The C-terminus of the prototypical M2 muscarinic receptor localizes to the mitochondria and regulates cell respiration under stress conditions.
Fasciani, I., Petragnano, F., Wang, Z., Edwards, R., Telugu, N., Pietrantoni, I., Zabel, U., Zauber, H., Grieben, M., Terzenidou, M.E., Di Gregorio, J., Pellegrini, C., Santini, S., Taddei, A.R., Pohl, B., Aringhieri, S., Carli, M., Aloisi, G., Marampon, F., Charlesworth, E., Roman, A., Diecke, S., Flati, V., Giorgi, F., Amicarelli, F., Tobin, A.B., Scarselli, M., Tokatlidis, K., Rossi, M., Lohse, M.J., Annibale, P. and Maggio, R.
PLoS Biology 22 (4): e3002582. 29 April 2024

PLoS Genetics

Mouse mutant phenotyping at scale reveals novel genes controlling bone mineral density.
Swan, A.L., Schütt, C., Rozman, J., Del Mar Muñiz Moreno, M., Brandmaier, S., Simon, M., Leuchtenberger, S., Griffiths, M., Brommage, R., Keskivali-Bond, P., Grallert, H., Werner, T., Teperino, R., Becker, L., Miller, G., Moshiri, A., Seavitt, J.R., Cissell, D.D., Meehan, T.F., Acar, E.F., Lelliott, C.J., Flenniken, A.M., Champy, M.F., Sorg, T., Ayadi, A., Braun, R.E., Cater, H., Dickinson, M.E., Flicek, P., Gallegos, J., Ghirardello, E.J., Heaney, J.D., Jacquot, S., Lally, C., Logan, J.G., Teboul, L., Mason, J., Spielmann, N., McKerlie, C., Murray, S.A., Nutter, L.M.J., Odfalk, K.F., Parkinson, H., Prochazka, J., Reynolds, C.L., Selloum, M., Spoutil, F., Svenson, K.L., Vales, T.S., Wells, S.E., White, J.K., Sedlacek, R., Wurst, W., Lloyd, K.K.C., Croucher, P.I., Fuchs, H., Williams, G.R., Bassett, D., Gailus-Durner, V., Herault, Y., Mallon, A.M., Brown, S.D.M., Mayer-Kuckuk, P. and Hrabe de Angelis, M.
PLoS Genetics 16 (12): e1009190. 28 December 2020

PLoS ONE

FGF/FGFR2 signaling regulates the generation and correct positioning of Bergmann glia cells in the developing mouse cerebellum.
Meier, F., Giesert, F., Delic, S., Faus-Kessler, T., Matheus, F., Simeone, A., Hoelter, S.M., Kühn, R., Vogt-Weisenhorn, D.M., Wurst, W. and Prakash, N.
PLoS ONE 9 (7): e101124. 1 July 2014

An RNAi-based approach to down-regulate a gene family in vivo.
Kim, J., Badaloni, A., Willert, T., Zimber-Strobl, U., Kühn, R., Wurst, W. and Kieslinger, M.
PLoS ONE 8 (11): e80312. 12 November 2013

Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector.
Merkl, C., Saalfrank, A., Riesen, N., Kühn, R., Pertek, A., Eser, S., Hardt, M.S., Kind, A., Saur, D., Wurst, W., Iglesias, A. and Schnieke, A.
PLoS ONE 8 (1): e55170. 31 January 2013

In vivo functional requirement of the mouse Ifitm1 gene for germ cell development, interferon mediated immune response and somitogenesis.
Klymiuk, I., Kenner, L., Adler, T., Busch, D.H., Boersma, A., Irmler, M., Fridrich, B., Gailus-Durner, V., Fuchs, H., Leitner, N., Mueller, M., Kuehn, R., Schlederer, M., Treise, I., de Angelis, M.H. and Beckers, J.
PLoS ONE 7 (10): e44609. 24 October 2012

Humanized c-Myc mouse.
Lehmann, F.M., Feicht, S., Helm, F., Maurberger, A., Ladinig, C., Zimber-Strobl, U., Kuehn, R., Mautner, J., Gerbitz, A. and Bornkamm, G.W.
PLoS ONE 7 (7): e42021. 31 July 2012

MAPK signaling determines anxiety in the juvenile mouse brain but depression-like behavior in adults.
Wefers, B., Hitz, C., Hoelter, S.M., Truembach, D., Hansen, J., Weber, P., Puetz, B., Deussing, J.M., de Angelis, M.H., Roenneberg, T., Zheng, F., Alzheimer, C., Silva, A., Wurst, W. and Kuehn, R.
PLoS ONE 7 (4): e35035. 18 April 2012

Pharmacogenomics Journal

Characterization of the melanocortin-4-receptor nonsense mutation W16X in vitro and in vivo.
Bolze, F., Rink, N., Brumm, H., Kühn, R., Mocek, S., Schwarz, A.E., Kless, C., Biebermann, H., Wurst, W., Rozman, J. and Klingenspor, M.
Pharmacogenomics Journal 13 (1): 80-93. February 2013

Proceedings of the National Academy of Sciences of the United States of America

Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line.
Chu, V.T., Graf, R., Wirtz, T., Weber, T., Favret, J., Li, X., Petsch, K., Tran, N.T., Sieweke, M.H., Berek, C., Kühn, R. and Rajewsky, K.
Proceedings of the National Academy of Sciences of the United States of America 113 (44): 12514-12519. 1 November 2016

Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides.
Wefers, B., Meyer, M., Ortiz, O., Hrabé de Angelis, M., Hansen, J., Wurst, W. and Kühn, R.
Proceedings of the National Academy of Sciences of the United States of America 110 (10): 3782-3787. 5 March 2013

Modeling disease mutations by gene targeting in one-cell mouse embryos.
Meyer, M., Ortiz, O., Hrabe de Angelis, M., Wurst, W. and Kuehn, R.
Proceedings of the National Academy of Sciences of the United States of America 109 (24): 9354-9359. 12 June 2012

Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases.
Meyer, M., de Angelis, M.H., Wurst, W. and Kühn, R.
Proceedings of the National Academy of Sciences of the United States of America 107 (34): 15022-15026. 24 August 2010

Progress in Immunology

Knock out mice models for immunodeficiency diseases.
Haas, W. and Kühn, R.
In: Progress in Immunology. Springer, 561-570 . ISBN 978-3-642-51481-4 1993

Research in Immunology

Gene targeting in immunology.
Kühn, R. and Müller, W.
Research in Immunology 148 (7): 447-449. September 1997

Interleukin-4-deficient mice.
Müller, W, Rajewsky, K and Kuehn, R.
Research in Immunology 144 (8): 637-638. October 1993

Science

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function.
Piwecka, M., Glažar, P., Hernandez-Miranda, L.R., Memczak, S., Wolf, S.A., Rybak-Wolf, A., Filipchyk, A., Klironomos, F., Cerda Jara, C.A., Fenske, P., Trimbuch, T., Zywitza, V., Plass, M., Schreyer, L., Ayoub, S., Kocks, C., Kühn, R., Rosenmund, C., Birchmeier, C. and Rajewsky, N.
Science 357 (6357): eaam8526. 22 September 2017

Inducible gene targeting in mice.
Kühn, R., Schwenk, F., Aguet, M. and Rajewsky, K.
Science 269 (5229): 1427-1429. 8 September 1995

MHC class I expression in mice lacking the proteasome subunit LMP-7.
Fehling, H.J., Swat, W., Laplace, C., Kühn, R., Rajewsky, K., Müller, U. and von Boehmer, H.
Science 265 (5176): 1234-1237. 26 August 1994

Resistance to murine acquired immunodeficiency syndrome (MAIDS).
Morawetz, R.A., Doherty, T.M., Giese, N.A., Hartley, J.W., Müller, W., Kühn, R., Rajewsky, K., Coffman, R. and Morse, H.C.
Science 265 (5169): 264-266. 8 July 1994

Generation and analysis of interleukin-4 deficient mice.
Kühn, R., Rajewsky, K. and Müller, W.
Science 254 (5032): 707-710. 1 November 1991

Science Advances

Precise CRISPR/Cas-mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells.
Tran, N.T., Danner, E., Li, X., Graf, R., Lebedin, M., de la Rosa, K., Kühn, R., Rajewsky, K. and Chu, V.T.
Science Advances 8 (22): eabm9106. 3 June 2022

Science Immunology

Precise CRISPR-Cas9 gene repair in autologous memory T cells to treat familial hemophagocytic lymphohistiocytosis.
Li, X., Wirtz, T., Weber, T., Lebedin, M., Lowenstein, E.D., Sommermann, T., Zach, A., Yasuda, T., de la Rosa, K., Chu, V.T., Schulte, J.H., Müller, I., Kocks, C. and Rajewsky, K.
Science Immunology 9 (92): eadi0042. 2 February 2024

Scientific Reports

The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4.
Funk, N., Munz, M., Ott, T., Brockmann, K., Wenninger-Weinzierl, A., Kühn, R., Vogt-Weisenhorn, D., Giesert, F., Wurst, W., Gasser, T. and Biskup, S.
Scientific Reports 9 (1): 4515. 14 March 2019

bioRxiv

Targeted H3K9 acetylation at lncSox1 promoter by cell type-specific epigenome editing promotes intermediate progenitor proliferation in developing mouse cortex.
Sokpor, Godwin, Ulmke, P.A., Nguyen, H.D., Pham, L., Kakani, P., Chu, V.T., Arnold, S.J., Brand-Saberi, B., Nguyen, H.P. and Tuoc, T.
bioRxiv : 2024.11.05.621758. 5 November 2024

Gene-edited primary muscle stem cells rescue dysferlin-deficient muscular dystrophy.
Escobar Fernandez, H., Di Francescantonio, S., Marg, A., Zhogov, A., Krishna, S., Metzler, E., Petkova, M., Daumke, O., Kühn, R. and Spuler, S.
bioRxiv : 2024.02.12.579813. 12 February 2024