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Signal requirements for growth and differentiation of activated murine B lymphocytes

Item Type:Article
Title:Signal requirements for growth and differentiation of activated murine B lymphocytes
Creators Name:Müller, W., Kühn, R., Goldmann, W., Tesch, H., Smith, F.I., Radbruch, A. and Rajewsky, K.
Abstract:Mouse B lymphocytes were stimulated at high cell concentrations with goat anti-IgM antibodies, which leads to the induction of B cell proliferation without the addition of any growth factors. After 48 hr, blast cells were purified and cultured at low cell concentrations. Proliferation and differentiation of purified B lymphocyte blasts is then dependent on the addition of either mitogens (e.g., LPS) or certain lymphokines derived from activated T cells or macrophages. One such lymphokine was isolated from supernatants of various activated T cells and characterized by gel filtration as a material with an apparent m.w. of 40,000 to 50,000, similar to BCGF II. It supports the proliferation of the B cell blasts and induces their differentiation into plaque-forming cells. Lymphokines such as BCGF I, interleukin 2, and BCDF gamma could neither maintain growth nor induce differentiation of B lymphocytes preactivated by goat anti-IgM.
Keywords:B-Lymphocytes, Cell Differentiation, Cell Division, Gel Chromatography, Growth Substances, High Pressure Liquid Chromatography, Interleukin-4, Lymphocyte Activation, Lymphokines, Macrophages, T-Lymphocytes, Animals, Inbred BALB C Mice, Inbred C57BL Mice, Mice
Source:Journal of Immunology
Publisher:American Association of Immunologists
Page Range:1213-1219
Date:August 1985
Official Publication:https://doi.org/10.4049/jimmunol.135.2.1213
PubMed:View item in PubMed

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