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Development of a species-specific RNA polymerase I-based shRNA expression vector

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Item Type:Article
Title:Development of a species-specific RNA polymerase I-based shRNA expression vector
Creators Name:Brenz Verca, M.S., Weber, P., Mayer, C., Graf, C., Refojo, D., Kühn, R., Grummt, I. and Lutz, B.
Abstract:RNA interference (RNAi) can be induced in vitro either by application of synthetic short interfering RNAs (siRNAs), or by intracellular expression of siRNAs or short hairpin RNAs (shRNAs) from transfected vectors. The most widely used promoters for siRNA/shRNA expression are based on polymerase III (Pol III)-dependent transcription. We developed an alternative vector for siRNA/shRNA expression, using a mouse RNA polymerase I (Pol I) promoter. Pol I-dependent transcription serves in cells for production of ribosomal RNA (rRNA), and as such, is ubiquitously and stably active in different cell types. As Pol I-dependent transcription is highly species-specific, Pol I-based system provides an important biosafety advantage with respect to silencing of genes with unknown functions.
Keywords:Cell Line, Genetic Promoter Regions, Genetic Transcription, Genetic Vectors, RNA Interference, RNA Polymerase I, Small Interfering RNA, Species Specificity, Untranslated RNA, Animals, Mice
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:e10
Date:January 2007
Official Publication:https://doi.org/10.1093/nar/gkl1045
PubMed:View item in PubMed

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