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Gene editing in one-cell embryos by zinc-finger and TAL nucleases

Item Type:Article
Title:Gene editing in one-cell embryos by zinc-finger and TAL nucleases
Creators Name:Wefers, B., Meyer, M., Hensler, S., Panda, S., Ortiz, O., Wurst, W. and Kühn, R.
Abstract:Gene targeting by sequence-specific nucleases in one-cell embryos provides an expedited mutagenesis approach in rodents. This technology has been recently established to create knockout and knockin mutants through sequence deletion or sequence insertion. This article provides protocols for the preparation and microinjection of nuclease mRNA and targeting vector DNA into fertilized mouse eggs. Furthermore, we provide guidelines for genotyping the desired mouse mutants.
Keywords:Pronucleus Injection, Gene Targeting, Mouse Mutant, Zinc-Finger Nuclease, TAL Nuclease, Homologous Recombination, Animals, Mice
Source:Current Protocols in Mouse Biology
Page Range:347-364
Date:December 2012
Official Publication:https://doi.org/10.1002/9780470942390.mo120177
PubMed:View item in PubMed

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