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Inducible gene targeting in mice

Item Type:Article
Title:Inducible gene targeting in mice
Creators Name:Kühn, R., Schwenk, F., Aguet, M. and Rajewsky, K.
Abstract:A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
Keywords:DNA Nucleotidyltransferases, DNA Polymerase I, GTP-Binding Proteins, Gene Targeting, Genetic Crosses, Genetic Promoter Regions, Genetic Recombination, Genetic Vectors, Integrases, Interferon-alpha, Interferon-beta, Liver, Myxovirus Resistance Proteins, Poly I-C, Proteins, Sequence Deletion, Viral Proteins, Animals, Mice
Publisher:American Association for the Advancement of Science
Page Range:1427-1429
Date:8 September 1995
Official Publication:https://doi.org/10.1126/science.7660125
PubMed:View item in PubMed

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