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Rapid generation of inducible mouse mutants

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Item Type:Article
Title:Rapid generation of inducible mouse mutants
Creators Name:Seibler, J., Zevnik, B., Küter-Luks, B., Andreas, S., Kern, H., Hennek, T., Rode, A., Heimann, C., Faust, N., Kauselmann, G., Schoor, M., Jaenisch, R., Rajewsky, K., Kühn, R. and Schwenk, F.
Abstract:We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.
Keywords:Cell Line, Gene Expression, Genetic Engineering, Genetic Recombination, Integrases, Knockout Mice, Mammalian Embryo, Recombinant Fusion Proteins, Steroid Receptors, Transgenic Mice, Viral Proteins, Animals, Mice
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:e12
Date:15 February 2003
Official Publication:https://doi.org/10.1093/nar/gng012
PubMed:View item in PubMed

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