Browse by Research Team

2024

Non-viral targeted insertion of large payloads into T cells.
Izsvak, Z.
Nature Biomedical Engineering 16 September 2024 (In Press)

Atypical KCNQ1/Kv7 channel function in a neonatal diabetes patient: hypersecretion preceded the failure of pancreatic β-cells.
Zhou, Z., Gong, M., Pande, A., Margineanu, A., Lisewski, U., Purfürst, B., Zhu, H., Liang, L., Jia, S., Froehler, S., Zeng, C., Kühnen, P., Khodaverdi, S., Krill, W., Röpke, T., Chen, W., Raile, K., Sander, M. and Izsvák, Z.
iScience 27 (7): 110291. 19 July 2024

Selection on synonymous sites: the unwanted transcript hypothesis.
Radrizzani, S., Kudla, G., Izsvák, Z. and Hurst, L.D.
Nature Reviews Genetics 25 (6): 431-448. June 2024

Advancing research and treatment: an overview of clinical trials in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and future perspectives.
Seton, K.A., Espejo-Oltra, J.A., Giménez-Orenga, K., Haagmans, R., Ramadan, D.J. and Mehlsen, J.
Journal of Clinical Medicine 13 (2): 325. 6 January 2024

2023

Staring at the onco-exaptation: the two-faced medley of an ancient retrovirus, HERVH.
Singh, M., Kondraskhina, A.M., Hurst, L.D. and Izsvák, Z.
Journal of Clinical Investigation 133 (14): e172278. 17 July 2023

A new human embryonic cell type associated with activity of young transposable elements allows definition of the inner cell mass.
Singh, M., Kondrashkina, A., Widmann, T.J., Cortes, J.L., Bansal, V., Wang, J., Römer, C., Garcia-Canadas, M., Garcia-Perez, J.L., Hurst, L.D. and Izsvak, Z.
PLoS Biology 21 (6): e3002162. 20 June 2023

HMGXB4 targets Sleeping Beauty transposition to germinal stem cells.
Devaraj, A., Singh, M., Narayanavari, S.A., Yong, G., Chen, J., Wang, J., Becker, M., Walisko, O., Schorn, A., Cseresznyés, Z., Raskó, T., Radscheit, K., Selbach, M., Ivics, Z. and Izsvak, Z.
International Journal of Molecular Sciences 24 (8): 7283. 14 April 2023

Enhanced biosafety of the Sleeping Beauty transposon system by using mRNA as source of transposase to efficiently and stably transfect retinal pigment epithelial cells.
Harmening, N., Johnen, S., Izsvák, Z., Ivics, Z., Kropp, M., Bascuas, T., Walter, P., Kreis, A., Pajic, B. and Thumann, G.
Biomolecules 13 (4): 658. 7 April 2023

Retrotransposon: a versatile player in human preimplantation development and health.
Qu, Y., Izsvák, Z. and Wang, J.
Life Medicine 2 (1): 1-3. February 2023

2022

Molecular and functional characterization of BDNF-overexpressing human retinal pigment epithelial cells established by sleeping beauty transposon-mediated gene transfer.
Mattern, L., Otten, K., Miskey, C., Fuest, M., Izsvák, Z., Ivics, Z., Walter, P., Thumann, G. and Johnen, S.
International Journal of Molecular Sciences 23 (21): 12982. 1 November 2022

GMP-grade manufacturing and quality control of a non-virally engineered advanced therapy medicinal product for personalized treatment of age-related macular degeneration.
Kropp, M., Harmening, N., Bascuas, T., Johnen, S., De Clerck, E., Fernández, V., Ronchetti, M., Cadossi, R., Zanini, C., Scherman, D., Ivics, Z., Marie, C., Izsvák, Z. and Thumann, G.
Biomedicines 10 (11): 2777. November 2022

A novel gene controls a new structure: piggyBac transposable element-derived 1, unique to mammals, controls mammal-specific neuronal paraspeckles.
Raskó, T., Pande, A., Radscheit, K., Zink, A., Singh, M., Sommer, C., Wachtl, G., Kolacsek, O., Inak, G., Szvetnik, A., Petrakis, S., Bunse, M., Bansal, V., Selbach, M., Orbán, T.I., Prigione, A., Hurst, L.D. and Izsvák, Z.
Molecular Biology and Evolution 39 (10): msac175. October 2022

Analyses of circRNA expression throughout the light-dark cycle reveal a strong regulation of (Cdr1as), associated with light entrainment in the SCN.
Ivanov, A., Mattei, D., Radscheit, K., Compagnion, A.C., Pett, J.P., Herzel, H.P., Paolicelli, R.C., Piwecka, M., Meyer, U. and Beule, D.
International Journal of Molecular Sciences 23 (20): 12347. October 2022

Functional indications for transposase domestications - characterization of the human piggyBac transposase derived (PGBD) activities.
Kolacsek, O., Wachtl, G., Fóthi, Á., Schamberger, A., Sándor, S., Pergel, E., Varga, N., Raskó, T., Izsvák, Z., Apáti, Á. and Orbán, T.I.
Gene 834 : 146609. 5 August 2022

Recapitulating early human development with 8C-like cells.
Yu, X., Liang, S., Chen, M., Yu, H., Li, R., Qu, Y., Kong, X., Guo, R., Zheng, R., Izsvak, Z., Sun, C., Yang, M. and Wang, J.
Cell Reports 39 (12): 110994. 21 June 2022

Safe and efficient in vivo hematopoietic stem cell transduction in nonhuman primates using HDAd5/35++ vectors.
Li, C., Wang, H., Gil, S., Germond, A., Fountain, C., Baldessari, A., Kim, J., Liu, Z., Georgakopoulou, A., Radtke, S., Raskó, T., Pande, A., Chiang, C., Chin, E., Yannaki, E., Izsvak, Z., Papayannopoulou, T., Kiem, H.P. and Lieber, A.
Molecular Therapy - Methods and Clinical Development 24 : 127-141. 10 March 2022

Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction.
Zywitza, V., Rusha, E., Shaposhnikov, D., Ruiz-Orera, J., Telugu, N., Rishko, V., Hayashi, M., Michel, G., Wittler, L., Stejskal, J., Holtze, S., Göritz, F., Hermes, R., Wang, J., Izsvák, Z., Colleoni, S., Lazzari, G., Galli, C., Hildebrandt, T.B., Hayashi, K., Diecke, S. and Drukker, M.
Scientific Reports 12 (1): 3100. 8 March 2022

Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells.
Yang, M., Yu, H., Yu, X., Liang, S., Hu, Y., Luo, Y., Izsvák, Z., Sun, C. and Wang, J.
Cell Stem Cell 29 (3): 400-418.e13. 3 March 2022

2021

Schizophrenia risk candidate protein ZNF804A interacts with STAT2 and influences interferon-mediated gene transcription in mammalian cells.
Klockmeier, K., Silva Ramos, E., Raskó, T., Martí Pastor, A. and Wanker, E.E.
Journal of Molecular Biology 433 (19): 167184. 17 September 2021

Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis.
Rössler, U., Hennig, A.F., Stelzer, N., Bose, S., Kopp, J., Søe, K., Cyganek, L., Zifarelli, G., Ali, S., von der Hagen, M., Strässler, E.T., Hahn, G., Pusch, M., Stauber, T., Izsvák, Z., Gossen, M., Stachelscheid, H. and Kornak, U.
Journal of Bone and Mineral Research 36 (8): 1621-1635. August 2021

Single-dose MGTA-145/plerixafor leads to efficient mobilization and in vivo transduction of HSCs with thalassemia correction in mice.
Li, C., Goncalves, K.A., Raskó, T., Pande, A., Gil, S., Liu, Z., Izsvák, Z., Papayannopoulou, T., Davis, J.C., Kiem, H.P. and Lieber, A.
Blood Advances 5 (5): 1239-1249. 9 March 2021

Isolation, culture, and genetic engineering of mammalian primary pigment epithelial cells for non-viral gene therapy.
Bascuas, T., Kropp, M., Harmening, N., Wong, B.M., Johnen, S., Izsvák, Z. and Thumann, G.
Journal of Visualized Experiments (168): e62145. 26 February 2021

Electroporation-based genetic modification of primary human pigment epithelial cells using the Sleeping Beauty transposon system.
Johnen, S., Harmening, N., Marie, C., Scherman, D., Izsvák, Z., Ivics, Z., Walter, P. and Thumann, G.
Journal of Visualized Experiments (168): e61987. February 2021

2020

Induction and analysis of oxidative stress in Sleeping Beauty transposon-transfected human retinal pigment epithelial cells.
Bascuas, T., Kropp, M., Harmening, N., Asrih, M., Izsvák, Z. and Thumann, G.
Journal of Visualized Experiments 2020 (166): e61957. 11 December 2020

Human endogenous retrovirus K rec forms a regulatory loop with MITF that opposes the progression of melanoma to an invasive stage.
Singh, M., Cai, H., Bunse, M., Feschotte, C. and Izsvák, Z.
Viruses 12 (11): 1303. 13 November 2020

Targeting CD33 in chemoresistant AML patient-derived xenografts by CAR-CIK cells modified with an improved SB transposon system.
Rotiroti, M.C., Buracchi, C., Arcangeli, S., Galimberti, S., Valsecchi, M.G., Perriello, V.M., Rasko, T., Alberti, G., Magnani, C.F., Cappuzzello, C., Lundberg, F., Pande, A., Dastoli, G., Introna, M., Serafini, M., Biagi, E., Izsvák, Z., Biondi, A. and Tettamanti, S.
Molecular Therapy 28 (9): 1974-1986. 2 September 2020

Curative in vivo hematopoietic stem cell gene therapy of murine thalassemia using large regulatory elements.
Wang, H., Georgakopoulou, A., Li, C., Liu, Z., Gil, S., Bashyam, A., Yannaki, E., Anagnostopoulos, A., Pande, A., Izsvák, Z., Papayannopoulou, T. and Lieber, A.
JCI Insight 5 (16): e139538. 20 August 2020

HMGXB4 targets Sleeping Beauty transposition to vertebrate germinal stem sells.
Devaraj, A., Singh, M., Narayanavari, S., Yong, G., Wang, J., Wang, J., Becker, M., Walisko, O., Schorn, A., Cseresznyés, Z., Grzela, D., Raskó, T., Selbach, M., Ivics, Z. and Izsvák, Z.
bioRxiv : 2020.06.15.145656. 15 June 2020

Nuclear inclusions of pathogenic ataxin-1 induce oxidative stress and perturb the protein synthesis machinery.
Laidou, S., Alanis-Lobato, G., Pribyl, J., Raskó, T., Tichy, B., Mikulasek, K., Tsagiopoulou, M., Oppelt, J., Kastrinaki, G., Lefaki, M., Singh, M., Zink, A., Chondrogianni, N., Psomopoulos, F., Prigione, A., Ivics, Z., Pospisilova, S., Skladal, P., Izsvák, Z., Andrade-Navarro, M.A. and Petrakis, S.
Redox Biology 32 : 101458. May 2020

Human endogenous retrovirus HERV-K(HML-2) RNA causes neurodegeneration through Toll-like receptors.
Dembny, P., Newman, A.G., Singh, M., Hinz, M., Szczepek, M., Krüger, C., Adalbert, R., Dzaye, O., Trimbuch, T., Wallach, T., Kleinau, G., Derkow, K., Richard, B.C., Schipke, C., Scheidereit, C., Stachelscheid, H., Golenbock, D., Peters, O., Coleman, M., Heppner, F.L., Scheerer, P., Tarabykin, V., Ruprecht, K., Izsvák, Z., Mayer, J. and Lehnardt, S.
JCI Insight 5 (7): e131093. 9 April 2020

Loss of Piccolo function in rats induces cerebellar network dysfunction and Pontocerebellar Hypoplasia type 3-like phenotypes.
Falck, J., Bruns, C., Hoffmann-Conaway, S., Straub, I., Plautz, E.J., Orlando, M., Munawar, H., Rivalan, M., Winter, Y., Izsvák, Z., Schmitz, D., Hamra, F.K., Hallermann, S., Curtis Garner, C. and Ackermann, F.
Journal of Neuroscience 40 (14): 2943-2959. 1 April 2020

A single amino acid switch converts the sleeping beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.
Kesselring, L., Miskey, C., Zuliani, C., Querques, I., Kapitonov, V., Laukó, A., Fehér, A., Palazzo, A., Diem, T., Lustig, J., Sebe, A., Wang, Y., Dinnyés, A., Izsvák, Z., Barabas, O. and Ivics, Z.
Nucleic Acids Research 48 (1): 316-331. 1 January 2020

2019

Preclinical evaluation of a cell-based gene therapy using the Sleeping Beauty transposon system in choroidal neovascularization.
Hernandez, M., Recalde, S., Garcia-Garcia, L., Bezunartea, J., Miskey, C., Johnen, S., Diarra, S., Sebe, A., Rodriguez-Madoz, J.R., Pouillot, S., Marie, C., Izsvák, Z., Scherman, D., Kropp, M., Prosper, F., Thumann, G., Ivics, Z., Garcia-Layana, A. and Fernandez-Robredo, P.
Molecular Therapy - Methods and Clinical Development 15 : 403-417. 13 December 2019

Critical role for piccolo in synaptic vesicle retrieval.
Ackermann, F., Schink, K.O., Bruns, C., Izsvák, Z., Hamra, F.K., Rosenmund, C. and Garner, C.C.
eLife 8 : e46629. 10 May 2019

A multiple Piccolino-RIBEYE interaction supports plate-shaped synaptic ribbons in retinal neurons.
Müller, T.M., Gierke, K., Joachimsthaler, A., Sticht, H., Izsvák, Z., Hamra, F.K., Fejtová, A., Ackermann, F., Garner, C.C., Kremers, J., Brandstätter, J.H. and Regus-Leidig, H.
Journal of Neuroscience 39 (14): 2606-2619. 3 April 2019

In vivo hematopoietic stem cell gene therapy ameliorates murine thalassemia intermedia.
Wang, H., Georgakopoulou, A., Psatha, N., Li, C., Capsali, C., Samal, H.B., Anagnostopoulos, A., Ehrhardt, A., Izsvák, Z., Papayannopoulou, T., Yannaki, E. and Lieber, A.
Journal of Clinical Investigation 129 (2): 598-615. 1 February 2019

2018

The genetic ancestry of modern Indus Valley populations from Northwest India.
Pathak, A.K., Kadian, A., Kushniarevich, A., Montinaro, F., Mondal, M., Ongaro, L., Singh, M., Kumar, P., Rai, N., Parik, J., Metspalu, E., Rootsi, S., Pagani, L., Kivisild, T., Metspalu, M., Chaubey, G. and Villems, R.
American Journal of Human Genetics 103 (6): 918-929. 6 December 2018

Ten things you should know about transposable elements.
Bourque, G., Burns, K.H., Gehring, M., Gorbunova, V., Seluanov, A., Hammell, M., Imbeault, M., Izsvák, Z., Levin, H.L., Macfarlan, T.S., Mager, D.L. and Feschotte, C.
Genome Biology 19 (1): 199. 19 November 2018

Improved molecular platform for the gene therapy of rare diseases by liver protein secretion.
Quiviger, M., Giannakopoulos, A., Verhenne, S., Marie, C., Stavrou, E.F., Vanhoorelbeke, K., Izsvák, Z., De Meyer, S.F., Athanassiadou, A. and Scherman, D.
European Journal of Medical Genetics 61 (11): 723-728. November 2018

A simple approach for multi-targeted shRNA-mediated inducible knockdowns using Sleeping Beauty vectors.
Fink, S., Zugelder, L., Roth, B., Brandt, E., Meloche, S., Izsvák, Z., Bargou, R.C. and Stühmer, T.
PLoS ONE 13 (10): e0205585. 19 October 2018

Mutant screen reveals the Piccolo's control over depression and brain-gonad crosstalk.
Medrano, G.A., Singh, M., Plautz, E.J., Good, L.B., Chapman, K.M., Chaudhary, J., Jaichander, P., Powell, H.M., Pudasaini, A., Shelton, J.M., Richardson, J.A., Xie, X.J., Ivics, Z., Braun, C., Ackermann, F., Garner, C.C., Izsvak, Z. and Hamra, F.K.
bioRxiv : 405985. 31 August 2018

Genome engineering using Sleeping Beauty transposition in vertebrates.
Chilkunda, S.S., Raghunathan, V., Narayanavari, S.A., Ivics, Z. and Izsvák, Z.
In: Genome editing and engineering. Cambridge University Press, Cambridge, 249-269. ISBN 9781316756300 July 2018

Integrating HDAd5/35++ vectors as a new platform for HSC gene therapy of hemoglobinopathies.
Li, C., Psatha, N., Wang, H., Singh, M., Samal, H.B., Zhang, W., Ehrhardt, A., Izsvák, Z., Papayannopoulou, T. and Lieber, A.
Molecular Therapy - Methods and Clinical Development 9 : 142-152. 15 June 2018

The antibiotic-free pFAR4 vector paired with the sleeping beauty transposon sSystem mediates efficient transgene delivery in human cells.
Pastor, M., Johnen, S., Harmening, N., Quiviger, M., Pailloux, J., Kropp, M., Walter, P., Ivics, Z., Izsvák, Z., Thumann, G., Scherman, D. and Marie, C.
Molecular Therapy - Nucleic Acids 11 : 57-67. 1 June 2018

The phylogenetically distinct early human embryo.
Singh, M., Widmann, T.J., Cortes, J.L., Schumann, G.G., Wunderlich, S., Martin, U., Garcia-Perez, J.L., Hurst, L.D. and Izsvak, Z.
bioRxiv : 318329. 18 May 2018

Efficient non-viral T cell engineering by Sleeping Beauty minicircles diminishing DNA toxicity and miRNAs silencing the endogenous TCR.
Clauss, J., Obenaus, M., Miskey, C., Ivics, Z., Izsvák, Z., Uckert, W. and Bunse, M.
Human Gene Therapy 29 (5): 569-584. May 2018

Efficient non-viral gene delivery into human hematopoietic stem cells by minicircle Sleeping Beauty transposon vectors.
Holstein, M., Mesa-Nuñez, C., Miskey, C., Almarza, E., Poletti, V., Schmeer, M., Grueso, E., Ordóñez Flores, J.C., Kobelt, D., Walther, W., Aneja, M.K., Geiger, J,, Bonig, H.B., Izsvák, Z., Schleef, M., Rudolph, C., Mavilio, F., Bueren, J.A., Guenechea, G. and Ivics, Z.
Molecular Therapy 26 (4): 1137-1153. 4 April 2018

A combined in vivo HSC transduction/selection approach results in efficient and stable gene expression in peripheral blood cells in mice.
Wang, H., Richter, M., Psatha, N., Li, C., Kim, J., Liu, J., Ehrhardt, A., Nilsson, S.K., Cao, B., Palmer, D., Ng, P., Izsvák, Z., Haworth, K.G., Kiem, H.P., Papayannopoulou, T. and Lieber, A.
Molecular Therapy - Methods and Clinical Development 8 : 52-64. 16 March 2018

High and long-term von Willebrand factor expression after Sleeping Beauty transposon-mediated gene therapy in a mouse model of severe von Willebrand disease.
Portier, I., Vanhoorelbeke, K., Verhenne, S., Pareyn, I., Vandeputte, N., Deckmyn, H., Goldenberg, D.S., Samal, H.B., Singh, M., Ivics, Z., Izsvák, Z. and De Meyer, S.F.
Journal of Thrombosis and Haemostasis 16 (3): 592-604. March 2018

2017

Long-term PEDF release in rat iris and retinal epithelial cells after Sleeping Beauty transposon-mediated gene delivery.
Garcia-Garcia, L., Recalde, S., Hernandez, M., Bezunartea, J., Rodriguez-Madoz, J.R., Johnen, S., Diarra, S., Marie, C., Izsvák, Z., Ivics, Z., Scherman, D., Kropp, M., Thumann, G., Prosper, F., Fernandez-Robredo, P. and Garcia-Layana, A.
Molecular Therapy - Nucleic Acids 9 : 1-11. 15 December 2017

Disturbed placental imprinting in preeclampsia leads to altered expression of DLX5, a human-specific early trophoblast marker.
Zadora, J., Singh, M., Herse, F., Przybyl, L., Haase, N., Golic, M., Yung, H.W., Huppertz, B., Cartwright, J.E., Whitley, G.S., Johnsen, G.M., Levi, G., Isbruch, A., Schulz, H., Luft, F.C., Müller, D.N., Staff, A.C., Hurst, L.D., Dechend, R. and Izsvák, Z.
Circulation 136 (19): 1824-1839. 7 November 2017

Gene therapy with the Sleeping Beauty transposon system.
Kebriaei, P., Izsvák, Z., Narayanavari, S.A., Singh, H. and Ivics, Z.
Trends in Genetics 33 (11): 852-870. November 2017

Wide awake and ready to move: 20 years of non-viral therapeutic genome engineering with the Sleeping Beauty transposon system.
Hodge, R., Narayanavari, S., Izsvák, Z. and Ivics, Z.
Human Gene Therapy 28 (10): 842-855. 1 October 2017

Generation of an immortalized mesenchymal stem cell line producing a secreted biosensor protein for glucose monitoring.
Siska, E.K., Weisman, I., Romano, J., Ivics, Z., Izsvák, Z., Barkai, U., Petrakis, S. and Koliakos, G.
PLoS ONE 12 (9): e0185498. 26 September 2017

Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side.
Hudecek, M., Izsvák, Z., Johnen, S., Renner, M., Thumann, G. and Ivics, Z.
Critical Reviews in Biochemistry and Molecular Biology 52 (4): 355-380. August 2017

How to tame an endogenous retrovirus: HERVH and the evolution of human pluripotency.
Römer, C., Singh, M., Hurst, L.D. and Izsvák, Z.
Current Opinion in Virology 25 : 49-58. August 2017

Downregulation of Plzf gene ameliorates metabolic and cardiac traits in the spontaneously hypertensive rat.
Liška, F., Landa, V., Zidek, V., Mlejnek, P., Šilhavý, J., Šimáková, M., Strnad, H., Trnovská, J., Škop, V., Kazdová, L., Starker, C.G., Voytas, D.F., Izsvák, Z., Mancini, M., Šeda, O., Křen, V. and Pravenec, M.
Hypertension 69 (6): 1084-1091. June 2017

Placental DAPK1 and autophagy marker LC3B-II are dysregulated by TNF-α in a gestational age-dependent manner.
Prokesch, A., Blaschitz, A., Bauer, T., Moser, G., Hiden, U., Zadora, J., Dechend, R., Herse, F. and Gauster, M.
Histochemistry and Cell Biology 147 (6): 695-705. June 2017

Human iPSC-derived neural progenitors are an effective drug discovery model for neurological mtDNA disorders.
Lorenz, C., Lesimple, P., Bukowiecki, R., Zink, A., Inak, G., Mlody, B., Singh, M., Semtner, M., Mah, N., Auré, K., Leong, M., Zabiegalov, O., Lyras, E.M., Pfiffer, V., Fauler, B., Eichhorst, J., Wiesner, B., Huebner, N., Priller, J., Mielke, T., Meierhofer, D., Izsvák, Z., Meier, J.C., Bouillaud, F., Adjaye, J., Schuelke, M., Wanker, E.E., Lombès, A. and Prigione, A.
Cell Stem Cell 20 (5): 659-674.e9. 4 May 2017

Long-term prevention of congenital thrombotic thrombocytopenic purpura in ADAMTS13 knockout mice by sleeping beauty transposon-mediated gene therapy.
Verhenne, S., Vandeputte, N., Pareyn, I., Izsvák, Z., Rottensteiner, H., Deckmyn, H., De Meyer, S.F. and Vanhoorelbeke, K.
Arteriosclerosis Thrombosis and Vascular Biology 37 (5): 836-844. May 2017

Localized irradiation of mouse legs using an image-guided robotic linear accelerator.
Kufeld, M., Escobar, H., Marg, A., Pasemann, D., Budach, V. and Spuler, S.
Annals of Translational Medicine 5 (7): 156. April 2017

Transposition of the bamboo Mariner-like element Ppmar1 in yeast.
Zhou, M.B., Hu, H., Miskey, C., Lazarow, K., Ivics, Z., Kunze, R., Yang, G., Izsvák, Z. and Tang, D.Q.
Molecular Phylogenetics and Evolution 109 : 367-374. April 2017

Sleeping Beauty transposon vectors for therapeutic applications: advances and challenges.
Narayanavari, S.A. and Izsvák, Z.
Cell and Gene Therapy Insights 3 (2): 131-158. 30 March 2017

Engineering of PEDF-expressing primary pigment epithelial cells by the SB transposon system delivered by pFAR4 plasmids.
Thumann, G., Harmening, N., Prat-Souteyrand, C., Marie, C., Pastor, M., Sebe, A., Miskey, C., Hurst, L., Diarra, S., Kropp, M., Walter, P., Scherman, D., Ivics, Z., Izsvák, Z. and Johnen, S.
Molecular Therapy - Nucleic Acids 6 : 302-314. 17 March 2017

Transposase.
Izsvák, Z. and Ivics, Z.
In: Reference Module in Life Sciences. Elsevier, 161-164. ISBN 978-0-12-809633-8 16 March 2017

Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition.
Wang, Y., Pryputniewicz-Dobrinska, D., Nagy, E.E., Kaufman, C.D., Singh, M., Yant, S., Wang, J., Dalda, A., Kay, M.A., Ivics, Z. and Izsvák, Z.
Nucleic Acids Research 45 (1): 311-326. 9 January 2017

Sleeping Beauty transposition: from biology to applications.
Narayanavari, S.A., Chilkunda, S.S., Ivics, Z. and Izsvák, Z.
Critical Reviews in Biochemistry and Molecular Biology 52 (1): 18-44. January 2017

2016

In vivo transduction of primitive mobilized hematopoietic stem cells after intravenous injection of integrating adenovirus vectors.
Richter, M., Saydaminova, K., Yumul, R., Krishnan, R., Liu, J., Nagy, E.E., Singh, M., Izsvák, Z., Cattaneo, R., Uckert, W., Palmer, D., Ng, P., Haworth, K.G., Kiem, H.P., Ehrhardt, A., Papayannopoulou, T. and Lieber, A.
Blood 128 (18): 2206-2217. 3 November 2016

Targeting of the Plzf gene in the rat by transcription activator-like effector nuclease results in caudal regression syndrome in spontaneously hypertensive rats.
Liška, F., Peterková, R., Peterka, M., Landa, V., Zidek, V., Mlejnek, P., Šilhavý, J., Šimáková, M., Křen, V., Starker, C.G., Voytas, D.F., Izsvák, Z. and Pravenec, M.
PLoS ONE 11 (10): e0164206. 11 October 2016

Structural determinants of sleeping beauty transposase activity.
Abrusán, G., Yant, S.R., Szilágyi, A., Marsh, J.A., Mátés, L., Izsvák, Z., Barabás, O. and Ivics, Z.
Molecular Therapy 24 (8): 1369-1377. August 2016

Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.
Voigt, F., Wiedemann, L., Zuliani, C., Querques, I., Sebe, A., Mátés, L., Izsvák, Z., Ivics, Z. and Barabas, O.
Nature Communications 7 : 11126. 30 March 2016

A Helitron transposon reconstructed from bats reveals a novel mechanism of genome shuffling in eukaryotes.
Grabundzija, I., Messing, S.A., Thomas, J., Cosby, R.L., Bilic, I., Miskey, C., Gogol-Döring, A., Kapitonov, V., Diem, T., Dalda, A., Jurka, J., Pritham, E.J., Dyda, F., Izsvák, Z. and Ivics, Z.
Nature Communications 7 : 10716. 2 March 2016

Generation of human induced pluripotent stem cell line from a patient with a long QT syndrome type 2.
Fatima, A., Ivanyuk, D., Herms, S., Heilmann-Heimbach, S., O'Shea, O., Chapman, C., Izsvák, Z., Farr, M., Hescheler, J. and Šarić, T.
Stem Cell Research 16 (2): 304-307. March 2016

Genome-wide profiling reveals remarkable parallels between insertion site selection properties of the MLV retrovirus and the piggyBac transposon in primary human CD4(+) T cells.
Gogol-Döring, A., Ammar, I., Gupta, S., Bunse, M., Miskey, C., Chen, W., Uckert, W., Schulz, T.F., Izsvák, Z. and Ivics, Z.
Molecular Therapy 24 (3): 592-606. March 2016

Sleeping Beauty transposon vectors in liver directed gene delivery of LDLR and VLDLR for gene therapy of familial hypercholesterolemia.
Turunen, T.A.K., Kurkipuro, J., Heikura, T., Vuorio, T., Hytönen, E., Izsvák, Z. and Ylä-Herttuala, S.
Molecular Therapy 24 (3): 620-635. March 2016

Identification and re-addressing of a transcriptionally permissive locus in the porcine genome.
Garrels, W., Mukherjee, A., Holler, S., Cleve, N., Talluri, T.R., Barg-Kues, B., Diederich, M., Köhler, P., Petersen, B., Lucas-Hahn, A., Niemann, H., Izsvák, Z., Ivics, Z. and Kues, W.A.
Transgenic Research 25 (1): 63-70. February 2016

Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression.
Wang, J., Singh, M., Sun, C., Besser, D., Prigione, A., Ivics, Z., Hurst, L.D. and Izsvák, Z.
Nature Protocols 11 (2): 327-346. February 2016

Full-length dysferlin transfer by the hyperactive Sleeping Beauty transposase restores dysferlin-deficient muscle.
Escobar, H., Schöwel, V., Spuler, S., Marg, A. and Izsvák, Z.
Molecular Therapy - Nucleic Acids 5 : e277. 19 January 2016

Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells.
Klawitter, S., Fuchs, N.V., Upton, K.R., Muñoz-Lopez, M., Shukla, R., Wang, J., Garcia-Cañadas, M., Lopez-Ruiz, C., Gerhardt, D.J., Sebe, A., Grabundzija, I., Merkert, S., Gerdes, P., Pulgarin, J.A., Bock, A., Held, U., Witthuhn, A., Haase, A., Sarkadi, B., Löwer, J., Wolvetang, E.J., Martin, U., Ivics, Z., Izsvák, Z., Garcia-Perez, J.L., Faulkner, G.J. and Schumann, G.G.
Nature Communications 7 : 10286. 8 January 2016

Pluripotency and the endogenous retrovirus HERVH: Conflict or serendipity?
Izsvák, Z., Wang, J., Singh, M., Mager, D.L. and Hurst, L.D.
BioEssays 38 (1): 109-117. January 2016

2015

Retrotransposition creates sloping shores: a graded influence of hypomethylated CpG islands on flanking CpG sites.
Grandi, F.C., Rosser, J.M., Newkirk, S.J., Yin, J., Jiang, X., Xing, Z., Whitmore, L., Bashir, S., Ivics, Z., Izsvák, Z., Ye, P., Yu, Y.E. and An, W.
Genome Research 25 (8): 1135-1146. August 2015

Transposon-based, targeted ex vivo gene therapy to treat age-related macular degeneration (TargetAMD).
Thumann, G., Perdikomati-Dahmen, M., Izsvák, Z., Ivics, Z., Garcia Layana, A., Ronchetti, M., Petrovski, G., Scherman, D., Aranda, P., Pouillot, S., Binder, S., Johnen, S. and van den Berg, J.
Human Gene Therapy Clinical Development 26 (2): 97-100. 18 June 2015

Sleeping Beauty transposition.
Ivics, Z. and Izsvák, Z.
Microbiology Spectrum 3 (2) April 2015

Antiangiogenic and neurogenic activities of Sleeping Beauty-mediated PEDF-transfected RPE cells in vitro and in vivo.
Johnen, S., Djalali-Talab, Y., Kazanskaya, O., Möller, T., Harmening, N., Kropp, M., Izsvák, Z., Walter, P. and Thumann, G.
BioMed Research International 2015 : 863845. 2015

Sleeping Beauty transposition.
Ivics, Z. and Izsvák, Z.
In: Mobile DNA III. ASM Press, Washington, DC, 851-872. ISBN 9781555819200 2015

2014

Primate-specific endogenous retrovirus-driven transcription defines naive-like stem cells.
Wang, J., Xie, G., Singh, M., Ghanbarian, A.T., Raskó, T., Szvetnik, A., Cai, H., Besser, D., Prigione, A., Fuchs, N.V., Schumann, G.G., Chen, W., Lorincz, M.C., Ivics, Z., Hurst, L.D. and Izsvák, Z.
Nature 516 (7531): 405-409. 18 December 2014

Genomic analysis of Sleeping Beauty transposon integration in human somatic cells.
Turchiano, G., Latella, M.C., Gogol-Döring, A., Cattoglio, C., Mavilio, F., Izsvák, Z., Ivics, Z. and Recchia, A.
PLoS ONE 9 (11): e112712. 12 November 2014

Human satellite cells have regenerative capacity and are genetically manipulable.
Marg, A., Escobar, H., Gloy, S., Kufeld, M., Zacher, J., Spuler, A., Birchmeier, C., Izsvák, Z. and Spuler, S.
Journal of Clinical Investigation 124 (10): 4257-4265. 1 October 2014

Gibbon genome and the fast karyotype evolution of small apes.
Carbone, L., Harris, R.A., Gnerre, S., Veeramah, K.R., Lorente-Galdos, B., Huddleston, J., Meyer, T.J., Herrero, J., Roos, C., Aken, B., Anaclerio, F., Archidiacono, N., Baker, C., Barrell, D., Batzer, M.A., Beal, K., Blancher, A., Bohrson, C.L., Brameier, M., Campbell, M.S., Capozzi, O., Casola, C., Chiatante, G., Cree, A., Damert, A., de Jong, P.J., Dumas, L., Fernandez-Callejo, M., Flicek, P., Fuchs, N.V., Gut, I., Gut, M., Hahn, M.W., Hernandez-Rodriguez, J., Hillier, L.W., Hubley, R., Ianc, B., Izsvák, Z., Jablonski, N.G., Johnstone, L.M., Karimpour-Fard, A., Konkel, M.K., Kostka, D., Lazar, N.H., Lee, S.L., Lewis, L.R., Liu, Y., Locke, D.P., Mallick, S., Mendez, F.L., Muffato, M., Nazareth, L.V., Nevonen, K.A., O'Bleness, M., Ochis, C., Odom, D.T., Pollard, K.S., Quilez, J., Reich, D., Rocchi, M., Schumann, G.G., Searle, S., Sikela, J.M., Skollar, G., Smit, A., Sonmez, K., ten Hallers, B., Terhune, E., Thomas, G.W.C., Ullmer, B., Ventura, M., Walker, J.A., Wall, J.D., Walter, L., Ward, M.C., Wheelan, S.J., Whelan, C.W., White, S., Wilhelm, L.J., Woerner, A.E., Yandell, M., Zhu, B., Hammer, M.F., Marques-Bonet, T., Eichler, E.E., Fulton, L., Fronick, C., Muzny, D.M., Warren, W.C., Worley, K.C., Rogers, J., Wilson, R.K. and Gibbs, R.A.
Nature 513 (7517): 195-201. 11 September 2014

Human somatic cell mutagenesis creates genetically tractable sarcomas.
Molyneux, S.D., Waterhouse, P.D., Shelton, D., Shao, Y.W., Watling, C.M., Tang, Q.L., Harris, I.S., Dickson, B.C., Tharmapalan, P., Sandve, G.K., Zhang, X., Bailey, S.D., Berman, H., Wunder, J.S., Izsvák, Z., Lupien, M., Mak, T.W. and Khokha, R.
Nature Genetics 46 (9): 964-972. September 2014

Excision efficiency is not strongly coupled to transgenic rate: cell type dependent transposition efficiency of Sleeping Beauty and piggyBac DNA transposons.
Kolacsek, O., Erdei, Z., Apáti, A., Sándor, S., Izsvák, Z., Ivics, Z., Sarkadi, B. and Orbán, T.I.
Human Gene Therapy Methods 25 (4): 241-252. August 2014

Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons.
Ivics, Z., Garrels, W., Mátés, L., Yau, T.Y., Bashir, S., Zidek, V., Landa, V., Geurts, A., Pravenec, M., Rülicke, T., Kues, W.A. and Izsvák, Z.
Nature Protocols 9 (4): 810-827. April 2014

Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons.
Ivics, Z., Hiripi, L., Hoffmann, O.I., Mátés, L., Yau, T.Y., Bashir, S., Zidek, V., Landa, V., Geurts, A., Pravenec, M., Rülicke, T., Bösze, Z. and Izsvák, Z.
Nature Protocols 9 (4): 794-809. April 2014

Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons.
Ivics, Z., Mátés, L., Yau, T.Y., Landa, V., Zidek, V., Bashir, S., Hoffmann, O.I., Hiripi, L., Garrels, W., Kues, W.A., Boesze, Z., Geurts, A., Pravenec, M., Rülicke, T. and Izsvák, Z.
Nature Protocols 9 (4): 773-793. April 2014

Suicidal autointegration of Sleeping Beauty and piggyBac transposons in eukaryotic cells.
Wang, Y., Wang, J., Devaraj, A., Singh, M., Jimenez Orgaz, A., Chen, J.X., Selbach, M., Ivics, Z. and Izsvák, Z.
PLoS Genetics 10 (3): e1004103. 13 March 2014

2013

Human endogenous retrovirus K (HML-2) RNA and protein expression is a marker for human embryonic and induced pluripotent stem cells.
Fuchs, N.V., Loewer, S., Daley, G.Q., Izsvák, Z., Löwer, J. and Löwer, R.
Retrovirology 10 (1): 115. 24 October 2013

Hybrid adeno-associated viral vectors utilizing transposase-mediated somatic integration for stable transgene expression in human cells.
Zhang, W., Solanki, M., Müther, N., Ebel, M., Wang, J., Sun, C., Izsvak, Z. and Ehrhardt, A.
PLoS ONE 8 (10): e76771. 8 October 2013

Integration profile and safety of an adenovirus hybrid-vector utilizing hyperactive Sleeping Beauty transposase for somatic integration.
Zhang, W., Muck-Hausl, M., Wang, J., Sun, C., Gebbing, M., Miskey, C., Ivics, Z., Izsvak, Z. and Ehrhardt, A.
PLoS ONE 8 (10): e75344. 4 October 2013

Impaired cell reprogramming in non-homologous end joining deficient cells.
Molina-Estevez, F.J., Lozano, M.L., Navarro, S., Torres, Y., Grabundzija, I., Ivics, Z., Samper, E., Bueren, J.A. and Guenechea, G.
Stem Cells 31 (8): 1726-1730. August 2013

Regulation of DNA transposition by CpG methylation and chromatin structure in human cells.
Jursch, T., Miskey, C., Izsvák, Z. and Ivics, Z.
Mobile DNA 4 (1): 15. 15 May 2013

Cell division promotes efficient retrotransposition in a stable L1 reporter cell line.
Xie, Y., Mates, L., Ivics, Z., Izsvák, Z., Martin, S.L. and An, W.
Mobile DNA 4 (1): 1-10. 6 March 2013

Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits.
Katter, K., Geurts, A.M., Hoffmann, O., Mátés, L., Landa, V., Hiripi, L., Moreno, C., Lazar, J., Bashir, S., Zidek, V., Popova, E., Jerchow, B., Becker, K., Devaraj, A., Walter, I., Grzybowksi, M., Corbett, M., Filho, A.R., Hodges, M.R., Bader, M., Ivics, Z., Jacob, H.J., Pravenec, M., Bosze, Z., Rülicke, T. and Izsvák, Z.
FASEB Journal 27 (3): 930-941. March 2013

Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells.
Grabundzija, I., Wang, J., Sebe, A., Erdei, Z., Kajdi, R., Devaraj, A., Steinemann, D., Szuhai, K., Stein, U., Cantz, T., Schambach, A., Baum, C., Izsvák, Z., Sarkadi, B. and Ivics, Z.
Nucleic Acids Research 41 (3): 1829-1847. 1 February 2013

Derivation and characterization of Sleeping Beauty transposon-mediated porcine induced pluripotent stem cells.
Kues, W.A., Herrmann, D., Barg-Kues, B., Meena Haridoss, S., Nowak-Imialek, M., Buchholz, T., Streeck, M., Grebe, A., Grabundzija, I., Merkert, S., Martin, U., Hall, V.J., Rasmussen, M.A., Ivics, Z., Maddox-Hyttel, P. and Niemann, H.
Stem Cells and Development 22 (1): 124-135. 1 January 2013

Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element.
Hozumi, A., Mita, K., Miskey, C., Mates, L., Izsvak, Z., Ivics, Z., Satake, H. and Sasakura, Y.
Developmental Dynamics 242 (1): 30-43. January 2013

2012

Generation of mouse induced pluripotent stem cells from different genetic backgrounds using Sleeping beauty transposon mediated gene transfer.
Muenthaisong, S., Ujhelly, O., Polgar, Z., Varga, E., Ivics, Z., Pirity, M.K. and Dinnyes, A.
Experimental Cell Research 318 (19): 2482-2489. 15 November 2012

Retargeting Sleeping Beauty transposon insertions by engineered zinc finger DNA-binding domains.
Voigt, K., Gogol-Doering, A., Miskey, C., Chen, W., Cathomen, T., Izsvak, Z. and Ivics, Z.
Molecular Therapy 20 (10): 1852-1862. October 2012

Retargeting transposon insertions by the adeno-associated virus Rep protein.
Ammar, I., Gogol-Doering, A., Miskey, C., Chen, W., Cathomen, T., Izsvak, Z. and Ivics, Z.
Nucleic Acids Research 40 (14): 6693-6712. 1 August 2012

Sleeping Beauty transposon-mediated transfection of retinal and iris pigment epithelial cells.
Johnen, S., Izsvak, Z., Stocker, M., Harmening, N., Salz, A.K., Walter, P. and Thumann, G.
Investigative Ophthalmology & Visual Science 53 (8): 4787-4796. 18 July 2012

Gateway compatible transposon vector to genetically modify human embryonic kidney and adipose-derived stromal cells.
Petrakis, S., Rasko, T., Mates, L., Ivics, Z., Izsvak, Z., Kouzi-Koliakou, K. and Koliakos, G.
Biotechnology Journal 7 (7): 891-897. July 2012

The Sleeping Beauty transposon system for clinical applications.
Swierczek, M., Izsvak, Z. and Ivics, Z.
Expert Opinion on Biological Therapy 12 (2): 139-153. February 2012

The sleeping beauty transposon toolbox.
Ammar, I., Izsvak, Z. and Ivics, Z.
Methods in Molecular Biology 859 : 229-240. 31 January 2012

2011

Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa.
Garrels, W., Holler, S., Taylor, U., Herrmann, D., Struckmann, C., Klein, S., Barg-Kues, B., Nowak-Imialek, M., Ehling, C., Rath, D., Ivics, Z., Niemann, H. and Kues, W.A.
PLoS ONE 6 (11): e27563. 16 November 2011

Recombinase technology for gene therapy.
Ivics, Z.
Current Gene Therapy 11 (5): 331. 1 October 2011

Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells.
Ivics, Z., Izsvak, Z., Medrano, G., Chapman, K.M. and Hamra, F.K.
Nature Protocols 6 (10): 1521-1535. October 2011

Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery.
Galla, M., Schambach, A., Falk, C.S., Maetzig, T., Kuehle, J., Lange, K., Zychlinski, D., Heinz, N., Brugman, M.H., Goehring, G., Izsvak, Z., Ivics, Z. and Baum, C.
Nucleic Acids Research 39 (16): 7147-7160. 1 September 2011

Nonviral gene delivery with the Sleeping Beauty transposon system.
Ivics, Z. and Izsvak, Z.
Human Gene Therapy 22 (9): 1043-1051. September 2011

The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor.
Jin, Z., Maiti, S., Huls, H., Singh, H., Olivares, S., Mates, L., Izsvak, Z., Ivics, Z., Lee, D.A., Champlin, R.E. and Cooper, L.J.
Gene Therapy 18 (9): 849-856. September 2011

Germline transgenic pigs by Sleeping Beauty transposition in porcine zygotes and targeted integration in the pig genome.
Garrels, W., Mates, L., Holler, S., Dalda, A., Taylor, U., Petersen, B., Niemann, H., Izsvak, Z., Ivics, Z. and Kues, W.A.
PLoS ONE 6 (8): e23573. 29 August 2011

Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells.
Moldt, B., Miskey, C., Staunstrup, N.H., Gogol-Doering, A., Bak, R.O., Sharma, N., Mates, L., Izsvak, Z., Chen, W., Ivics, Z. and Mikkelsen, J.G.
Molecular Therapy 19 (8): 1499-1510. August 2011

Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells.
Ivics, Z., Izsvak, Z., Chapman, K.M. and Hamra, F.K.
Methods 53 (4): 356-365. April 2011

Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers.
Kolacsek, O., Krizsik, V., Schamberger, A., Erdei, Z., Apati, A., Varady, G., Mates, L., Izsvak, Z., Ivics, Z., Sarkadi, B. and Orban, T.I.
Mobile DNA 2 (1): 5. 3 March 2011

Transgene Expression durch zytoplasmatische Injektion von Plasmiden und Transposon-basierten Konstrukten in Saeugerembryonen [Transgenetic expression by cytoplasmic injection of plasmids and trans­poson-based constructs in mammalian embryos].
Garrels, W., Holler, S., Ivics, Z., Niemann, H. and Kues, W.A.
Zuechtungskunde 83 (1): 47-67. January 2011

Insertional engineering of chromosomes with Sleeping Beauty transposition: an overview.
Grabundzija, I., Izsvak, Z. and Ivics, Z.
In: Mammalian Chromosome Engineering. Methods in Molecular Biology, 738 . Springer / Humana Press, 69-85. 2011

2010

The expanding universe of transposon technologies for gene and cell engineering.
Ivics, Z. and Izsvak, Z.
Mobile DNA 1 (1): 25. 7 December 2010

Herpes simplex virus/Sleeping Beauty Vector-based embryonic gene transfer using the HSB5 mutant: loss of apparent transposition hyperactivity in vivo.
de Silva, S., Mastrangelo, M.A., Lotta, L.T., Burris, C.A., Izsvak, Z., Ivics, Z. and Bowers, W.J.
Human Gene Therapy 21 (11): 1603-1613. 19 November 2010

A DNA-transposon-based approach to functional screening in neural stem cells.
Albieri, I., Onorati, M., Calabrese, G., Moiana, A., Biasci, D., Badaloni, A., Camnasio, S., Spiliotopoulos, D., Ivics, Z., Cattaneo, E. and Consalez, G.G.
Journal of Biotechnology 150 (1): 11-21. 1 October 2010

Novel hyperactive transposons for genetic modification of induced puripotent and adult stem cells: a non-viral paradigm for coaxed differentiation.
Belay, E., Matrai, J., Acosta-Sanchez, A., Ma, L., Quattrocelli, M., Mates, L., Sancho-Bru, P., Geraerts, M., Yan, B., Vermeesch, J., Rincon, M.Y., Samara-Kuko, E., Ivics, Z., Verfaillie, C.M., Sampaolesi, M., Izsvak, Z., Vandendriessche, T. and Chuah, M.K.
Stem Cells 28 (10): 1760-1771. October 2010

Translating Sleeping Beauty transposition into cellular therapies: Victories and challenges.
Izsvak, Z., Hackett, P.B., Cooper, L.J. and Ivics, Z.
BioEssays 32 (9): 756-767. September 2010

Novel strong tissue specific promoter for gene expression in human germ cells.
Kuzmin, D., Gogvadze, E., Kholodenko, R., Grzela, D.P., Mityaev, M., Vinogradova, T., Kopantzev, E., Malakhova, G., Suntsova, M., Sokov, D., Ivics, Z. and Buzdin, A.
BMC Biotechnology 10 (1): 58. 17 August 2010

Repetitive elements and genome instability.
Ivics, Z. and Izsvak, Z.
Seminars in Cancer Biology 20 (4): 197-199. August 2010

Comparative analysis of transposable element vector systems in human cells.
Grabundzija, I., Irgang, M., Mates, L., Belay, E., Matrai, J., Gogol-Doering, A., Kawakami, K., Chen, W., Ruiz, P., Chuah, M.K., Vandendriessche, T., Izsvak, Z. and Ivics, Z.
Molecular Therapy 18 (6): 1200-1209. June 2010

Generating knockout rats by transposon mutagenesis in spermatogonial stem cells.
Izsvak, Z., Froehlich, J., Grabundzija, I., Shirley, J.R., Powell, H.M., Chapman, K.M., Ivics, Z. and Hamra, F.K.
Nature Methods 7 (6): 443-445. June 2010

Efficient conditional and promoter-specific in vivo expression of cDNAs of choice by taking advantage of recombinase-mediated cassette exchange using FlEx gene traps.
Schebelle, L., Wolf, C., Stribl, C., Javaheri, T., Schnuetgen, F., Ettinger, A., Ivics, Z., Hansen, J., Ruiz, P., von Melchner, H., Wurst, W. and Floss, T.
Nucleic Acids Research 38 (9): e106. May 2010

A novel active endogenous retrovirus family contributes to genome variability in rat inbred strains.
Wang, Y., Liska, F., Gosele, C., Sedova, L., Kren, V., Krenova, D., Ivics, Z., Huebner, N. and Izsvak, Z.
Genome Research 20 (1): 19-27. January 2010

Unique functions of repetitive transcriptomes.
Schumann, G.G., Gogvadze, E.V., Osanai-Futahashi, M., Kuroki, A., Muenk, C., Fujiwara, H., Ivics, Z. and Buzdin, A.A.
International Review of Cell and Molecular Biology 285 : 115-188. 2010

2009

Efficient stable gene transfer into human cells by Sleeping Beauty transposon vectors.
Izsvak, Z., Chuah, M.K., VandenDriessche, T. and Ivics, Z.
Methods 49 (3): 287-297. November 2009

Emerging potential of transposons for gene therapy and generation of induced pluripotent stem cells.
Vandendriessche, T., Ivics, Z., Izsvak, Z. and Chuah, M.K.
Blood 114 (8): 1461-1468. 20 August 2009

Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system.
Xue, X., Huang, X., Nodland, S.E., Mates, L., Ma, L., Izsvak, Z., Ivics, Z., Lebien, T.W., McIvor, R.S., Wagner, J.E. and Zhou, X.
Blood 114 (7): 1319-1330. 13 August 2009

Hybrid lentivirus-transposon vectors with a random integration profile in human cells.
Staunstrup, N.H., Moldt, B., Mates, L., Villesen, P., Jakobsen, M., Ivics, Z., Izsvak, Z. and Mikkelsen, J.G.
Molecular Therapy 17 (7): 1205-1214. July 2009

Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates.
Mates, L., Chuah, M.K., Belay, E., Jerchow, B., Manoj, N., Acosta-Sanchez, A., Grzela, D.P., Schmitt, A., Becker, K., Matrai, J., Ma, L., Samara-Kuko, E., Gysemans, C., Pryputniewicz, D., Miskey, C., Fletcher, B., Vandendriessche, T., Ivics, Z. and Izsvak, Z.
Nature Genetics 41 (6): 753-761. June 2009

Transposon-mediated genome manipulation in vertebrates.
Ivics, Z., Li, M.A., Mates, L., Boeke, J.D., Nagy, A., Bradley, A. and Izsvak, Z.
Nature Methods 6 (6): 415-422. June 2009

Genomic parasites and genome evolution.
Ivics, Z.
Genome Biology 10 (4): 306. 15 April 2009

Molecular domestication of transposable elements: From detrimental parasites to useful host genes.
Sinzelle, L., Izsvak, Z. and Ivics, Z.
Cellular and Molecular Life Sciences 66 (6): 1073-1093. March 2009

Applying a "double-feature" promoter to identify cardiomyocytes differentiated from human embryonic stem cells following transposon-based gene delivery.
Orban, T.I., Apati, A., Nemeth, A., Varga, N., Krizsik, V., Schamberger, A., Szebenyi, K., Erdei, Z., Varady, G., Karaszi, E., Homolya, L., Nemet, K., Gocza, E., Miskey, C., Mates, L., Ivics, Z., Izsvak, Z. and Sarkadi, B.
Stem Cells 27 (5): 1077-1087. 20 February 2009

Interactions of transposons with the cellular DNA repair machinery.
Izsvak, Z., Wang, Y. and Ivics, Z.
Genome Dynamics and Stability 4 : 133-176. 2009

Transposable elements as plasmid-based vectors for long-term gene transfer into tumors.
Ohlfest, J.R., Ivics, Z. and Izsvak, Z.
Methods in Molecular Biology 542 (Part 2): 105-116. 2009

Transposon-host cell interactions in the regulation of Sleeping Beauty transposition.
Walisko, O., Jursch, T., Izsvak, Z. and Ivics, Z.
Genome Dynamics and Stability 4 : 109-132. 2009

2008

Targeted gene insertion for molecular medicine.
Voigt, K., Izsvak, Z. and Ivics, Z.
Journal of Molecular Medicine 86 (11): 1205-1219. November 2008

Progress and prospects in rat genetics: a community view.
Aitman, T.J., Critser, J.K., Cuppen, E., Dominiczak, A., Fernandez-Suarez, X.M., Flint, J., Gauguier, D., Geurts, A.M., Gould, M., Harris, P.C., Holmdahl, R., Huebner, N., Izsvak, Z., Jacob, H.J., Kuramoto, T., Kwitek, A.E., Marrone, A., Mashimo, T., Moreno, C., Mullins, J., Mullins, L., Olsson, T., Pravenec, M., Riley, L., Saar, K., Serikawa, T., Shull, J.D., Szpirer, C., Twigger, S.N., Voigt, B. and Worley, K.C.
Nature Genetics 40 (5): 516-22. May 2008

Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes.
Sinzelle, L., Kapitonov, V.V., Grzela, D.P., Jursch, T., Jurka, J., Izsvak, Z. and Ivics, Z.
Proceedings of the National Academy of Sciences of the United States of America 105 (12): 4715-4720. 25 March 2008

Transcriptional activities of the Sleeping Beauty transposon and shielding its genetic cargo with insulators.
Walisko, O., Schorn, A., Rolfs, F., Devaraj, A., Miskey, C., Izsvak, Z. and Ivics, Z.
Molecular Therapy 16 (2): 359-369. February 2008

Insertional mutagenesis of the mouse germline with Sleeping Beauty transposition.
Takeda, J., Izsvak, Z. and Ivics, Z.
Methods in Molecular Biology 435 : 109-125. 2008

2007

Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.
Mates, L., Izsvak, Z. and Ivics, Z.
Genome Biology 8 Suppl 1 : S1. 31 October 2007

The Ancient Mariner Sails Again: Transposition of the Human Hsmar1 Element by a Reconstructed Transposase and Activities of the SETMAR Protein on Transposon Ends.
Miskey, C., Papp, B., Mates, L., Sinzelle, L., Keller, H., Izsvak, Z. and Ivics, Z.
Molecular and Cellular Biology 27 (12): 4589-4600. 15 June 2007

Targeted Sleeping Beauty transposition in human cells.
Ivics, Z., Katzer, A., Stuewe, E.E., Fiedler, D., Knespel, S. and Izsvak, Z.
Molecular Therapy 15 (6): 1137-1144. June 2007

2006

Transposons for gene therapy!
Ivics, Z. and Izsvak, Z.
Current Gene Therapy 6 (5): 593-607. October 2006

Hungary's 'reforms' are threatening basic science.
Izsvak, Z., Ivics, Z. and Mates, L.
Nature 443 (7110): 394. 28 September 2006

Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1.
Walisko, O., Izsvak, Z., Szabo, K., Kaufman, C.D., Herold, S. and Ivics, Z.
Proceedings of the National Academy of Sciences of the United States of America 103 (11): 4062-4067. 14 March 2006

Transposons.
Ivics, Z., Walisko, O. and Izsvak, Z.
In: Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine ; Vol. 2. Springer, Berlin [u.a.], 1915-1919. ISBN 3-540-44244-8 2006

2005

Transposon-based targeting system [Transposon-System zur gezielten Integration].
Ivics, Z. and Izsvak, Z.
EP1594971 ; EP1594972 ; EP1594973 ; WO0469994 ; WO0469995 ; WO0470042. 16 November 2005

Frog Prince transposon-based RNAi vectors mediate efficient gene knockdown in human cells.
Kaufman, C.D., Izsvak, Z., Katzer, A. and Ivics, Z.
Journal of RNAi and Gene Silencing 1 (2): 97-104. October 2005

Sleeping Beauty hits them all: transposon-mediated saturation mutagenesis in the mouse germline.
Izsvak, Z. and Ivics, Z.
Nature Methods 2 (10): 735-736. October 2005

DNA transposons in vertebrate functional genomics.
Miskey, C., Izsvak, Z., Kawakami, K. and Ivics, Z.
Cellular and Molecular Life Sciences 62 (6): 629-641. March 2005

A whole lotta jumpin' goin' on: new transposon tools for vertebrate functional genomics.
Ivics, Z. and Izsvak, Z.
Trends in Genetics 21 (1): 8-11. 1 January 2005

2004

Development of hyperactive Sleeping Beauty transposon vectors by mutational analysis.
Zayed, H., Izsvak, Z., Walisko, O. and Ivics, Z.
Molecular Therapy 9 (2): 292-304. 1 February 2004

Sleeping Beauty transposition: biology and applications for molecular therapy.
Izsvak, Z. and Ivics, Z.
Molecular Therapy 9 (2): 147-156. 1 February 2004

Healing the wounds inflicted by Sleeping Beauty transposition by double-strand break repair in mammalian somatic cells.
Izsvak, Z., Stuewe, E.E., Fiedler, D., Katzer, A., Jeggo, P.A. and Ivics, Z.
Molecular Cell 13 (2): 279-290. 1 January 2004

The Sleeping Beauty transposable element: evolution, regulation and genetic applications.
Ivics, Z., Kaufman, C.D., Zayed, H., Miskey, C., Walisko, O. and Izsvak, Z.
Current Issues in Molecular Biology 6 (1): 43-55. 1 January 2004

Transposable elements for transgenesis and insertional mutagenesis in vertebrates: a contemporary review of experimental strategies.
Ivics, Z. and Izsvak, Z.
In: Mobile Genetic Elements: Protocols and Genomic Applications. Methods in Molecular Biology ; 260, 260 . Humana Press, Totowa, NJ, 255-276. ISBN 1-58829-007-7 1 January 2004

2003

Frog Prince, ein Transposonvektor fuer den Gentransfer bei Wirbeltieren [The Frog Prince, a transposon vector for gene transfer in vertebrates].
Miskey, C., Izsvak, Z. and Ivics, Z.
DE10224242A1 ; EP1507865 ; WO03100070. 11 December 2003

The Frog Prince: a reconstructed transposon from Rana pipiens with high transpositional activity in vertebrate cells.
Miskey, C., Izsvak, Z., Plasterk, R.H. and Ivics, Z.
Nucleic Acids Research 31 (23): 6873-6881. 1 December 2003

The DNA-bending protein HMGB1 is a cellular cofactor of Sleeping Beauty transposition.
Zayed, H., Izsvak, Z., Khare, D., Heinemann, U. and Ivics, Z.
Nucleic Acids Research 31 (9): 2313-2322. 1 May 2003

2002

Common physical properties of DNA affecting target site selection of Sleeping Beauty and other Tc1/mariner transposable elements.
Vigdal, T.J., Kaufman, C.D., Izsvak, Z., Voytas, D.F. and Ivics, Z.
Journal of Molecular Biology 323 (3): 441-452. 25 October 2002

Involvement of a bifunctional, paired-like DNA-binding domain and a transpositional enhancer in Sleeping Beauty transposition.
Izsvak, Z., Khare, D., Behlke, J., Heinemann, U., Plasterk, R.H. and Ivics, Z.
Journal of Biological Chemistry 277 (37): 34581-34588. 13 September 2002

2000

Sleeping Beauty, a wide host-range transposon vector for genetic transformation in vertebrates.
Izsvak, Z., Ivics, Z. and Plasterk, R.H.
Journal of Molecular Biology 302 : 93-102. 8 September 2000

Somatic integration and long-term transgene expression in normal and haemophilic mice using a DNA transposon system.
Yant, S.R., Meuse, L., Chiu, W., Ivics, Z., Izsvak, Z. and Kay, M.A.
Nature Genetics 25 (1): 35-41. 1 May 2000

1999

Resident aliens: the Tc1/mariner superfamily of transposable elements.
Plasterk, R.H., Izsvak, Z. and Ivics, Z.
Trends in Genetics 15 : 326-332. 1 August 1999

Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell.
Hackett, B.P., Clark, J.K., Dupuy, J.A., Ekker, C.S., Largaespada, A.D., Ivics, Z. and Izsvak, Z.
EP1034258 ; WO9925817. 27 May 1999

Development of genetic tools for transgenic animals.
Hackett, P.B., Izsvak, Z., Ivics, Z. and Caldovic, L.
In: Transgenic Animals in Agriculture. CAB International, Wallingford, UK, 19-35. ISBN 9780851992938 1 January 1999

Short inverted-repeat transposable elements in teleost fish and implications for a mechanism of their amplification.
Izsvak, Z., Ivics, Z., Shimoda, N., Mohn, D., Okamoto, H. and Hackett, P.B.
Journal of Molecular Evolution 48 : 13-21. 1 January 1999

1998

Elements transposables: des outils permettant la mutagenese et le transgenese chez les vertebres [Transposable elements as tools from mutagenesis and transgenesis of vertebrates].
Plasterk, R.H., Van Luenen, H., Vos, C., Ivics, Z., Izsvak, Z. and Fischer, S.
Pathologie Biologie 46 : 674-675. 1 November 1998

Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells.
Luo, G., Ivics, Z., Izsvak, Z. and Bradley, A.
Proceedings of the National Academy of Sciences of the United States of America 95 (18): 10769-10773. 1 September 1998

1997

Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells.
Ivics, Z., Hackett, P.B., Plasterk, R.H. and Izsvak, Z.
Cell 91 (4): 501-510. 14 November 1997

Repetitive elements and their genetic applications in zebrafish.
Izsvak, Z., Ivics, Z. and Hackett, P.B.
Biochemistry and Cell Biology 75 (5): 507-523. 1 October 1997

Cloning and characterization of the genes of the CeqI restriction-modification system.
Izsvak, Z., Jobbagy, Z., Takacs, I. and Duda, E.
International Journal of Biochemistry and Cell Biology 29 : 895-900. 1 June 1997

Family of plasmid vectors for the expression of beta-galactosidase fusion proteins in eukaryotic cells.
Ivics, Z. and Izsvak, Z.
Biotechniques 22 : 254-258. 1 February 1997

1996

Identification of functional domains and evolution of Tc1-like transposable elements.
Ivics, Z., Izsvak, Z., Minter, A. and Hackett, P.B.
Proceedings of the National Academy of Sciences of the United States of America 93 (10): 5008-5013. 14 May 1996

DANA elements: a family of composite, tRNA-derived short interspersed DNA elements associated with mutational activities in zebrafish (Danio rerio).
Izsvak, Z., Ivics, Z., Garcia-Estefania, D., Fahrenkrug, S.C. and Hackett, P.B.
Proceedings of the National Academy of Sciences of the United States of America 93 (3): 1077-1081. 6 February 1996

1995

Characterization of a Tc1-like transposable element in zebrafish (Danio rerio).
Izsvak, Z., Ivics, Z. and Hackett, P.B.
Molecular and General Genetics 247 (3): 312-322. 10 May 1995

1993

Two-stage ligation-mediated PCR enhances the detection of integrated transgenic DNA.
Izsvak, Z. and Ivics, Z.
Biotechniques 15, 5 : 814-818. 1 November 1993

Enhanced incorporation of transgenic DNA into zebrafish chromosomes by a retroviral integration protein.
Ivics, Z., Izsvak, Z. and Hackett, P.B.
Molecular Marine Biology and Biotechnology 2 (3): 162-173. June 1993

1992

Purification and characterization of CeqI restriction endonuclease.
Izsvak, Z., Jobbagy, Z. and Duda, E.
Zeitschrift fuer Naturforschung C, A Journal of Biosciences 47 : 830-834. 1 November 1992

Positive co-operative interaction between the subunits of CeqI restriction endonuclease.
Jobbagy, Z., Izsvak, Z. and Duda, E.
Biochemical Journal 286 : 85-88. 15 August 1992

1989

‘Star’ activity and complete loss of specificity of CeqI endonuclease.
Izsvak, Z. and Duda, E.
Biochemical Journal 258 (1): 301-303. February 1989