Browse by Journal Title

2025

Concurrent profiling of localized transcriptome and RNA dynamics in neurons by spatial SLAMseq.
Dantsuji, S. and Chekulaeva, M.
Methods in Molecular Biology 2863 : 297-317. 2025

2024

3D spheroid and organoid models to study neuroinfection of RNA viruses.
Dos Reis, V.P., Cirksena, K., Rybak-Wolf, A., Seeger, B., Herker, E. and Gerold, G.
Methods in Molecular Biology 2824 : 409-424. August 2024

Single-cell RNA-sequencing of RVFV infection.
Wyler, E.
Methods in Molecular Biology 2824 : 361-372. August 2024

Identifying chromosome movement patterns during meiosis using ChroMo.
Pinto-Cruz, J., Correas, M., Mendoza-Madrigal, R., León-Periñán, D. and Fernández-Álvarez, A.
Methods in Molecular Biology 2818 : 271-288. 2024

2023

A DNA origami-based biointerface to interrogate the spatial requirements for sensitized T-Cell antigen recognition.
Hellmeier, J., Platzer, R., Huppa, J.B. and Sevcsik, E.
Methods in Molecular Biology 2654 : 277-302. 28 April 2023

Combining metabolic pulse labeling and quantitative proteomics to monitor protein synthesis upon viral infection.
Bogdanow, B., Katsimani, N., Liu, F. and Selbach, M.
Methods in Molecular Biology 2610 : 149-165. 2023

Physics-based polymer models to probe chromosome structure in single molecules.
Conte, M., Chiariello, A.M., Bianco, S., Esposito, A., Abraham, A. and Nicodemi, M.
Methods in Molecular Biology 2655 : 57-66. 2023

Thioester and oxyester linkages in the ubiquitin system.
Ferri-Blazquez, A., Jarosch, E. and Sommer, T.
Methods in Molecular Biology 2602 : 3-18. 2023

Resolving crosstalk between signaling pathways using mathematical modeling and time-resolved single cell data.
Konrath, F., Loewer, A. and Wolf, J.
Methods in Molecular Biology 2634 : 267-284. 2023

Visualizing MyoD oscillations in muscle stem cells.
Lahmann, I. and Birchmeier, C.
Methods in Molecular Biology 2640 : 259-276. 2023

Concomitant sequencing of accessible chromatin and mitochondrial genomes in single cells using mtscATAC-seq.
Ludwig, L.S. and Lareau, C.A.
Methods in Molecular Biology 2611 : 269-282. 2023

Getting to the root of branched ubiquitin chains: a review of current methods and functions.
Waltho, A. and Sommer, T.
Methods in Molecular Biology 2602 : 19-38. 2023

Gene editing in mouse zygotes using the CRISPR/Cas9 system.
Wefers, B., Wurst, W. and Kühn, R.
Methods in Molecular Biology 2631 : 207-230. 2023

2022

Analysis of single-cell transcriptome data in drosophila.
Yousefian, S., Musillo, M.J. and Bageritz, J.
Methods in Molecular Biology 2540 (3): 93-111. 19 August 2022

Claudin-targeted suicide gene therapy for claudin-overexpressing tumor cells by using modified clostridium perfringens enterotoxin (CPE).
Beier, L.S., Piontek, J., Piontek, A., Protze, J., Kobelt, D. and Walther, W.
Methods in Molecular Biology 2521 (3): 173-188. 23 June 2022

Generation of redirected engineered human chimeric antigen receptor (CAR) T cells.
Bunse, M. and Höpken, U.E.
Methods in Molecular Biology 2521 (3): 67-83. 23 June 2022

TCR and CAR engineering of primary human T cells.
Edes, I., Clauss, J., Stahn, R., Sada Japp, A. and Lorenz, F.K.M.
Methods in Molecular Biology 2521 (3): 85-93. 23 June 2022

A brief introduction to current cancer gene therapy.
Kobelt, D., Pahle, J. and Walther, W.
Methods in Molecular Biology 2521 (3): 1-21. 23 June 2022

Gene gun Her2/neu DNA vaccination: evaluation of vaccine efficacy in a syngeneic Her2/neu mouse tumor model.
Nguyen-Hoai, T., Hohn, O., Pezzutto, A. and Westermann, J.
Methods in Molecular Biology 2521 (3): 129-154. 23 June 2022

TCR gene therapy for cancer.
Rhein, S. and Çakmak-Görür, N.
Methods in Molecular Biology 2521 (3): 95-128. 23 June 2022

Capillary gel electrophoresis (CGE) for quality control of plasmid DNA in gene therapy: quality control of 20 years stored GMP-grade plasmid DNA.
Schmeer, M., Schleef, M., Shankar, R., Kobelt, D. and Walther, W.
Methods in Molecular Biology 2521 (3): 317-328. 23 June 2022

Examination of the contributions of maternal/placental-derived galectin-1 to pregnancy outcome.
Borowski, S., Freitag, N., Urban, I., Michel, G., Barrientos, G. and Blois, S.M.
Methods in Molecular Biology 2442 : 603-619. 2022

A polymer physics model to dissect genome organization in healthy and pathological phenotypes.
Conte, M., Fiorillo, L., Bianco, S., Chiariello, A.M., Esposito, A., Musella, F., Flora, F., Abraham, A. and Nicodemi, M.
Methods in Molecular Biology 2301 : 307-316. 2022

Real-time measurements of intracellular cAMP gradients using FRET-based cAMP nanorulers.
Kayser, C., Lohse, M.J. and Bock, A.
Methods in Molecular Biology 2483 : 1-13. 2022

Disruptors of AKAP-dependent protein-protein interactions.
Walker-Gray, R., Pallien, T., Miller, D.C., Oder, A., Neuenschwander, M., von Kries, J.P., Diecke, S. and Klussmann, E.
Methods in Molecular Biology 2483 : 117-139. 2022

2021

From tube to structure: SPA Cryo-EM workflow using apoferritin as an example.
Diebolder, C.A., Dillard, R.S. and Renault, L.
Methods in Molecular Biology 2305 (2305): 229-256. 6 May 2021

Metastasis : methods and protocols.
Stein, U.S., ed.
Methods in Molecular Biology, 2294 . Springer / Humana Press, New York, NY. ISBN 978-1-0716-1349-8 2021

Preclinical MRI of the kidney : methods and protocols.
Pohlmann, A. and Niendorf, T., eds.
Methods in Molecular Biology, 2216 . Springer / Humana Press, New York, NY. ISBN 978-1-0716-0977-4 2021

From operating table to laboratory bench: the path toward metastasis research from the clinical setting.
Alberto Vilchez, M. and Rau, B.
Methods in Molecular Biology 2294 : 325-333. 2021

Quantitative assessment of renal perfusion and oxygenation by invasive probes: basic concepts.
Cantow, K., Evans, R.G., Grosenick, D., Gladytz, T., Niendorf, T., Flemming, B. and Seeliger, E.
Methods in Molecular Biology 2216 : 89-107. 2021

Monitoring renal hemodynamics and oxygenation by invasive probes: experimental protocol.
Cantow, K., Ladwig-Wiegard, M., Flemming, B., Pohlmann, A., Niendorf, T. and Seeliger, E.
Methods in Molecular Biology 2216 : 327-347. 2021

Quantitative analysis of renal perfusion by arterial spin labeling.
Chuang, K.H., Kober, F. and Ku, M.C.
Methods in Molecular Biology 2216 : 655-666. 2021

Renal blood flow using arterial spin labeling (ASL) MRI: experimental protocol and principles.
Chuang, K.H., Meier, M., Fernández-Seara, M.A., Kober, F. and Ku, M.C.
Methods in Molecular Biology 2216 : 443-453. 2021

A bioinformatic pipeline to identify biomarkers for metastasis formation from RNA sequencing data.
Dahlmann, M. and Stein, U.S.
Methods in Molecular Biology 2294 : 221-238. 2021

Hardware considerations for preclinical magnetic resonance of the kidney.
Delgado, P.R., Küstermann, E., Kühne, A., Millward, J.M., Niendorf, T., Pohlmann, A. and Meier, M.
Methods in Molecular Biology 2216 : 131-155. 2021

Genome-wide analysis of actively translated open reading frames using riboTaper/ORFquant.
Harnett, D., Meerdink, E., Calviello, L., Sydow, D. and Ohler, U.
Methods in Molecular Biology 2252 : 331-346. 2021

Bioenergetic profiling of human pluripotent stem cells.
Inak, G., Henke, M.T. and Prigione, A.
Methods in Molecular Biology 2277 : 391-403. 2021

Analysis of renal diffusion-weighted imaging (DWI) using apparent diffusion coefficient (ADC) and intravoxel incoherent motion (IVIM) models.
Jerome, N.P. and Periquito, J.S.
Methods in Molecular Biology 2216 : 611-635. 2021

Preparation and monitoring of small animals in renal MRI.
Kaucsar, T., Hosszu, A., Seeliger, E., Reimann, H.M. and Fekete, A.
Methods in Molecular Biology 2216 : 45-55. 2021

Real-time cell migration monitoring to analyze drug synergism in the scratch assay using the IncuCyte system.
Kobelt, D., Walther, W. and Stein, U.S.
Methods in Molecular Biology 2294 : 133-142. 2021

Noninvasive renal perfusion measurement using arterial spin labeling (ASL) MRI: basic concept.
Ku, M.C., Fernández-Seara, M.A., Kober, F. and Niendorf, T.
Methods in Molecular Biology 2216 : 229-239. 2021

Fluorine ((19)F) MRI for assessing inflammatory cells in the kidney: experimental protocol.
Ku, M.C., Schreiber, A., Delgado, P.R., Boehm-Sturm, P., Kettritz, R., Niendorf, T., Pohlmann, A. and Waiczies, S.
Methods in Molecular Biology 2216 : 495-507. 2021

Preparation of ex vivo rodent phantoms for developing, testing, and training MR imaging of the kidney and other organs.
Millward, J.M., Periquito, J.S., Delgado, P.R., Prinz, C., Niendorf, T. and Waiczies, S.
Methods in Molecular Biology 2216 : 75-85. 2021

Preface.
Niendorf, T. and Pohlmann, A.
Methods in Molecular Biology 2216 : v-vi. 2021

Renal MRI diffusion: experimental protocol.
Periquito, J.S., Meier, M., Niendorf, T., Pohlmann, A. and Jerome, N.P.
Methods in Molecular Biology 2216 : 419-428. 2021

Analysis protocols for MRI mapping of the blood oxygenation-sensitive parameters T(2)* and T(2) in the kidney.
Periquito, J.S., Starke, L., Santos, C.M., Freitas, A.C., Loução, N., Polo, P.G., Nunes, R.G., Niendorf, T. and Pohlmann, A.
Methods in Molecular Biology 2216 : 591-610. 2021

Recommendations for preclinical renal MRI: a comprehensive open-access protocol collection to improve training, reproducibility, and comparability of studies.
Pohlmann, A., Back, S.J., Fekete, A., Friedli, I., Hectors, S., Jerome, N.P., Ku, M.C., Longo, D.L., Meier, M., Millward, J.M., Periquito, J.S., Seeliger, E., Serai, S.D, Waiczies, S., Sourbron, S., Laustsen, C. and Niendorf, T.
Methods in Molecular Biology 2216 : 3-23. 2021

Essential practical steps for MRI of the kidney in experimental research.
Pohlmann, A., Periquito, J.S. and Niendorf, T.
Methods in Molecular Biology 2216 : 349-367. 2021

Experimental protocol for MRI mapping of the blood oxygenation-sensitive parameters T(2)* and T(2) in the kidney.
Pohlmann, A., Zhao, K., Fain, S.B., Prasad, P.V. and Niendorf, T.
Methods in Molecular Biology 2216 : 403-417. 2021

Subsegmentation of the kidney in experimental MR images using morphology-based regions-of-interest or multiple-layer concentric objects.
Riazy, L., Milani, B., Periquito, J.S., Cantow, K., Niendorf, T., Pruijm, M., Seeliger, E. and Pohlmann, A.
Methods in Molecular Biology 2216 : 549-564. 2021

Bioluminescence in G protein-coupled receptors drug screening using nanoluciferase and halo-tag technology.
Schihada, H., Nemec, K., Lohse, M.J. and Maiellaro, I.
Methods in Molecular Biology 2268 : 137-147. 2021

Data preparation protocol for low signal-to-noise ratio fluorine-19 MRI.
Starke, L., Niendorf, T. and Waiczies, S.
Methods in Molecular Biology 2216 : 711-722. 2021

Denoising for improved parametric MRI of the kidney: protocol for nonlocal means filtering.
Starke, L., Tabelow, K., Niendorf, T. and Pohlmann, A.
Methods in Molecular Biology 2216 : 565-576. 2021

Preface.
Stein, U.S.
Methods in Molecular Biology 2294 : v. 2021

Functional imaging using fluorine ((19)F) MR methods: basic concepts.
Waiczies, S., Prinz, C., Starke, L., Millward, J.M., Delgado, P.R., Rosenberg, J., Nazaré, M., Waiczies, H., Pohlmann, A. and Niendorf, T.
Methods in Molecular Biology 2216 : 279-299. 2021

Patient-derived xenografts from solid tumors (PDX) for models of metastasis.
Wulf-Goldenberg, A., Hoffmann, J., Becker, M., Brzezicha, B. and Walther, W.
Methods in Molecular Biology 2294 : 43-58. 2021

2019

Genotoxic stress-induced senescence.
Fan, D.N.Y. and Schmitt, C.A.
Methods in Molecular Biology 1896 : 93-105. 2019

Evolution of protein domain architectures.
Forslund, S.K., Kaduk, M. and Sonnhammer, E.L.L.
Methods in Molecular Biology 1910 : 469-504. 2019

RNA tomography for spatially resolved transcriptomics (Tomo-seq).
Holler, K. and Junker, J.P.
Methods in Molecular Biology 1920 : 129-141. 2019

Lyophilization of synthetic gene carriers.
Kasper, J.C., Hedtrich, S. and Friess, W.
Methods in Molecular Biology 1943 (2): 211-225. 2019

Using SMOG 2 to simulate complex biomolecular assemblies.
Levi, M., Bandarkar, P., Yang, H., Wang, A., Mohanty, U., Noel, J.K. and Whitford, P.C.
Methods in Molecular Biology 2022 : 129-151. 2019

Imaging glioma progression by intravital microscopy.
Stanchi, F., Matsumoto, K. and Gerhardt, H.
Methods in Molecular Biology 1862 : 227-243. 2019

Scalable workflows and reproducible data analysis for genomics.
Strozzi, F., Janssen, R., Wurmus, R., Crusoe, M.R., Githinji, G., Di Tommaso, P., Belhachemi, D., Möller, S., Smant, G., de Ligt, J. and Prins, P.
Methods in Molecular Biology 1910 : 723-745. 2019

Efficient gene editing of human induced pluripotent stem cells using CRISPR/Cas9.
Yumlu, S., Bashir, S., Stumm, J. and Kühn, R.
Methods in Molecular Biology 1961 : 137-151. 2019

2018

A filter retardation assay facilitates the detection and quantification of heat-stable, amyloidogenic mutant huntingtin aggregates in complex biosamples.
Ast, A., Schindler, F., Buntru, A., Schnoegl, S. and Wanker, E.E.
Methods in Molecular Biology 1780 : 31-40. 2018

Imaging of endothelial cell dynamic behavior in zebrafish.
Coxam, B. and Gerhardt, H.
Methods in Molecular Biology 1846 : 181-195. 2018

Integrated proteogenomic approach for identifying degradation motifs in eukaryotic cells.
Geffen, Y., Appleboim, A., Gardner, R.G. and Ravid, T.
Methods in Molecular Biology 1844 : 121-136. 2018

Single-molecule fluorescence in situ hybridization (FISH) of circular RNA CDR1as.
Kocks, C., Boltengagen, A., Piwecka, M., Rybak-Wolf, A. and Rajewsky, N.
Methods in Molecular Biology 1724 : 77-96. 2018

Cardiac MRI in small animals.
Ku, M.C., Huelnhagen, T., Niendorf, T. and Pohlmann, A.
Methods in Molecular Biology 1718 : 269-284. 2018

Assessment of blood brain barrier leakage with gadolinium-enhanced MRI.
Ku, M.C., Waiczies, S., Niendorf, T. and Pohlmann, A.
Methods in Molecular Biology 1718 : 395-408. 2018

Systematic detection of poly(A)(+) RNA-interacting proteins and their differential binding.
Milek, M. and Landthaler, M.
Methods in Molecular Biology 1649 : 405-417. 2018

Metabolic labeling of newly synthesized RNA with 4sU to in parallel assess RNA transcription and decay.
Sun, W. and Chen, W.
Methods in Molecular Biology 1720 : 25-34. 2018

2017

Förster resonance energy transfer to study TCR-pMHC interactions in the immunological synapse.
Schütz, G.J. and Huppa, J.B.
In: The immune synapse. Methods in Molecular Biology, 1584 . Springer Nature, New York, NY, USA, 207-229. ISBN 978-1-4939-8327-8 3 March 2017

Bioinformatics analysis of functional associations of PTMs.
Minguez, P. and Bork, P.
Methods in Molecular Biology 1558 (1558): 303-320. 2 February 2017

CRISPR/Cas9-mediated in vitro mutagenesis in GC-like B cells.
Chu, V.T., Graf, R. and Rajewsky, K.
Methods in Molecular Biology 1623 : 135-145. 2017

Detecting markers of therapy-induced senescence in cancer cells.
Fan, D.N.Y. and Schmitt, C.A.
Methods in Molecular Biology 1534 : 41-52. 2017

Imaging activity-dependent signaling dynamics at the neuronal synapse using FRET-based biosensors.
Farsi, Z. and Woehler, A.
Methods in Molecular Biology 1538 : 261-275. 2017

Development of mouse model systems of germinal center lymphomas.
Kabrani, E. and Sander, S.
Methods in Molecular Biology 1623 : 233-242. 2017

Epigenetics and control of RNAs.
Maatz, H., van Heesch, S., Kreuchwig, F., Faber, A., Adami, E., Hubner, N. and Heinig, M.
Methods in Molecular Biology 1488 : 217-237. 2017

Evaluation of endothelial dysfunction in vivo.
Todiras, M., Alenina, N. and Bader, M.
Methods in Molecular Biology 1527 : 355-367. 2017

2016

Isolation of eosinophils from the lamina propria of the murine small intestine.
Berek, C., Beller, A. and Chu, V.T.
Methods in Molecular Biology 1422 : 213-221. 2016

Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes.
Burridge, P.W., Diecke, S., Matsa, E., Sharma, A., Wu, H. and Wu, J.C.
Methods in Molecular Biology 1353 : 119-130. 2016

A pipeline for PAR-CLIP data analysis.
Jens, M.
Methods in Molecular Biology 1358 : 197-207. 2016

Generating sample-specific databases for mass spectrometry-based proteomic analysis by using RNA sequencing.
Luge, T. and Sauer, S.
Methods in Molecular Biology 1394 : 219-232. 2016

Genome editing in mice using TALE nucleases.
Wefers, B., Brandl, C., Ortiz, O., Wurst, W. and Kühn, R.
Methods in Molecular Biology 1338 : 229-243. 2016

Identifying RBP targets with RIP-seq.
Wessels, H.H., Hirsekorn, A., Ohler, U. and Mukherjee, N.
Methods in Molecular Biology 1358 : 141-152. 2016

2015

Assessment of renal hemodynamics and oxygenation by simultaneous magnetic resonance imaging (MRI) and quantitative invasive physiological measurements.
Cantow, K., Arakelyan, K., Seeliger, E., Niendorf, T. and Pohlmann, A.
Methods in Molecular Biology 1397 : 129-154. 17 December 2015

Magnetic resonance imaging (MRI) analysis of ischemia/reperfusion in experimental acute renal injury.
Pohlmann, A., Arakelyan, K., Seeliger, E. and Niendorf, T.
Methods in Molecular Biology 1397 : 113-127. 17 December 2015

Screening for small molecule disruptors of AKAP-PKA interactions.
Schächterle, C., Christian, F., Fernandes, J.M.P. and Klussmann, E.
Methods in Molecular Biology 1294 : 151-166. 2 March 2015

Induced pluripotent stem cells (iPSCs) for modeling mitochondrial DNA disorders.
Prigione, A.
Methods in Molecular Biology 1265 : 349-356. 6 January 2015

An ex vivo model for studying mitochondrial trafficking in neurons.
Bros, H., Niesner, R. and Infante-Duarte, C.
Methods in Molecular Biology 1264 : 465-472. 2015

RNA interference for antimetastatic therapy.
Dahlmann, M. and Stein, U.
Methods in Molecular Biology 1317 : 153-165. 2015

Gene gun Her2/neu DNA vaccination: evaluation of vaccine efficacy in a syngeneic Her2/neu mouse tumor model.
Nguyen-Hoai, T., Pezzutto, A. and Westermann, J.
Methods in Molecular Biology 1317 : 17-37. 2015

Oncoleaking: use of the pore-forming clostridium perfringens enterotoxin (CPE) for suicide gene therapy.
Pahle, J., Aumann, J., Kobelt, D. and Walther, W.
Methods in Molecular Biology 1317 : 69-85. 2015

Assessing the bioenergetic profile of human pluripotent stem cells.
Pfiffer, V. and Prigione, A.
Methods in Molecular Biology 1264 : 279-288. 2015

2014

In vivo stable isotope labeling by amino acids in Drosophila melanogaster.
Sury, M.D., Chen, J.X. and Selbach, M.
Methods in Molecular Biology 1188 : 85-93. 8 July 2014

Experimental applications of TNF-reporter mice with far-red fluorescent label.
Shebzukhov, Y.V., Kuchmiy, A.A., Kruglov, A.A., Zipp, F., Siffrin, V. and Nedospasov, S.A.
Methods in Molecular Biology 1155 : 151-162. 10 April 2014

Second generation codon optimized minicircle (CoMiC) for nonviral reprogramming of human adult fibroblasts.
Diecke, S., Lisowski, L., Kooreman, N.G. and Wu, J.C.
Methods in Molecular Biology 1181 : 1-13. 1 January 2014

Pancreas development ex vivo: culturing embryonic pancreas explants on permeable culture inserts, with fibronectin-coated glass microwells, or embedded in three-dimensional Matrigel(TM).
Shih, H.P. and Sander, M.
Methods in Molecular Biology 1210 : 229-37. 1 January 2014

IPG strip-based peptide fractionation for shotgun proteomics.
Eravci, M., Sommer, C. and Selbach, M.
Methods in Molecular Biology 1156 : 67-77. 2014

A method for the generation of TCR retrogenic mice.
Kieback, E., Hilgenberg, E. and Fillatreau, S.
Methods in Molecular Biology 1193 : 117-126. 2014

Whole-mount in situ hybridization using DIG-labeled probes in planarian.
Rybak-Wolf, A. and Solana, J.
Methods in Molecular Biology 1211 : 41-51. 2014

2013

Target validation in mice by constitutive and conditional RNAi.
Kleinhammer, A., Wurst, W. and Kühn, R.
Methods in Molecular Biology 986 : 307-323. 23 January 2013

A genetic strategy for the analysis of individual axon morphologies in cGMP signalling mutant mice.
Schmidt, H., Ter-Avetisyan, G. and Rathjen, F.G.
Methods in Molecular Biology 1020 : 193-204. 2013

2012

In vivo quantitative proteome profiling: planning and evaluation of SILAC experiments.
Kirchner, M. and Selbach, M.
Methods in Molecular Biology 893 : 175-199. 7 May 2012

The sleeping beauty transposon toolbox.
Ammar, I., Izsvak, Z. and Ivics, Z.
Methods in Molecular Biology 859 : 229-240. 31 January 2012

Evolution of protein domain architectures.
Forslund, K. and Sonnhammer, E.L.L.
Methods in Molecular Biology 856 : 187-216. 2012

An overview of the analysis of next generation sequencing data.
Gogol-Doering, A. and Chen, W.
Methods in Molecular Biology 802 : 249-257. 2012

Genes involved in the metastatic cascade of medullary thyroid tumours.
Schreiber, C., Vormbrock, K. and Ziebold, U.
Methods in Molecular Biology 878 : 217-228. 2012

The investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants, and other in vivo methods for PPI mapping.
Suter, B. and Wanker, E.E.
Methods in Molecular Biology 812 : v-vi. 2012

Live cell imaging of G protein-coupled receptors.
Teichmann, A., Schmidt, A., Wiesner, B., Oksche, A. and Schuelein, R.
Methods in Molecular Biology 897 : 139-169. 2012

2011

Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach.
Ebner, O.A. and Selbach, M.
Methods in Molecular Biology 725 : 315-331. 2011

Generating conditional knockout mice.
Friedel, R.H., Wurst, W., Wefers, B. and Kuehn, R.
Methods in Molecular Biology 693 : 205-231. 2011

Insertional engineering of chromosomes with Sleeping Beauty transposition: an overview.
Grabundzija, I., Izsvak, Z. and Ivics, Z.
In: Mammalian Chromosome Engineering. Methods in Molecular Biology, 738 . Springer / Humana Press, 69-85. 2011

High-throughput subcellular protein localization using transfected-cell arrays: subcellular protein localization using cell arrays.
Hu, Y. and Janitz, M.
Methods in Molecular Biology 706 : 53-72. 2011

2010

Rat models of cardiovascular diseases.
Bader, M.
Methods in Molecular Biology 597 : 403-414. 2010

Calibration of microarray gene-expression data.
Binder, H., Preibisch, S. and Berger, H.
Methods in Molecular Biology 576 : 375-407. 2010

Jet-injection of short hairpin RNA-encoding vectors into tumor cells.
Walther, W., Stein, U. and Lage, H.
Methods in Molecular Biology 629 : 123-139. 2010

CryoFISH: fluorescence in situ hybridization on ultrathin cryosections.
Xie, S.Q., Lavitas, L.M. and Pombo, A.
Methods in Molecular Biology 659 : 219-230. 2010

2009

Gene knockout protocols.
Wurst, W. and Kuehn, R., eds.
Methods in Molecular Biology, 530 . Springer / Humana Press. ISBN 978-1-934115-26-8 2009

Generation of shRNA transgenic mice.
Hitz, C., Steuber-Buchberger, P., Delic, S., Wurst, W. and Kuehn, R.
Methods in Molecular Biology 530 : 101-129. 2009

Overview on mouse mutagenesis.
Kühn, R. and Wurst, W.
Methods in Molecular Biology 530 : 1-12. 2009

Use of SNP-arrays for ChIP assays: computational aspects.
Muro, E.M., McCann, J.A., Rudnicki, M.A. and Andrade-Navarro, M.A.
Methods in Molecular Biology 567 : 145-154. 2009

Transposable elements as plasmid-based vectors for long-term gene transfer into tumors.
Ohlfest, J.R., Ivics, Z. and Izsvak, Z.
Methods in Molecular Biology 542 (Part 2): 105-116. 2009

Nonviral jet-injection technology for intratumoral in vivo gene transfer of naked DNA.
Walther, W., Fichtner, I., Schlag, P.M. and Stein, U.S.
Methods in Molecular Biology 542 : 195-208. 2009

2008

Insertional mutagenesis of the mouse germline with Sleeping Beauty transposition.
Takeda, J., Izsvak, Z. and Ivics, Z.
Methods in Molecular Biology 435 : 109-125. 2008

2007

Immunohistochemical detection of beta-galactosidase or green fluorescent protein on tissue sections.
Seymour, P.A. and Sander, M.
Methods in Molecular Biology 411 : 13-23. 13 September 2007

StemBase: a resource for the analysis of stem cell gene expression data.
Porter, C.J., Palidwor, G.A., Sandie, R., Krzyzanowski, P.M., Muro, E.M., Perez-Iratxeta, C. and Andrade-Navarro, M.A.
Methods in Molecular Biology 407 : 137-148. 2007

2006

Comparative promoter analysis in vertebrate genomes with the CORG workbench.
Dieterich, C. and Vingron, M.
Methods in Molecular Biology 338 : 105-118. 2006

Derivation, maintenance, and characterization of rat embryonic stem cells in vitro.
Schulze, M., Ungefroren, H., Bader, M. and Faendrich, F.
Methods in Molecular Biology 329 : 45-58. 2006

2005

E2F transcription factors and pRb pocket proteins in cell cycle progression.
Hauck, L. and von Harsdorf, R.
Methods in Molecular Biology 296 : 239-245. 1 January 2005

2004

Applications of interspersed repeat sequence polymerase chain reaction.
Zimdahl, H., Goesele, C., Kreitler, T. and Knoblauch, M.
Methods in Molecular Biology 255 : 113-129. 2004

2003

Nanoelectrospray tandem mass spectrometry and sequence similarity searching for identification of proteins from organisms with unknown genomes.
Shevchenko, A., Sunyaev, S., Liska, A., Bork, P. and Shevchenko, A.
Methods in Molecular Biology 211 : 221-234. 1 January 2003

Huntingtin fragments form aggresome-like inclusion bodies in mammalian cells.
Boeddrich, A., Lurz, R. and Wanker, E.E.
Methods in Molecular Biology 232 : 217-229. 2003

Differentiation of human antigen-presenting dendritic cells from CD34+ hematopoietic stem cells in vitro.
Ju, X.S. and Zenke, M.
Methods in Molecular Biology 215 : 399-407. 2003

2002

Fourier transform infrared spectroscopy of calcium-binding proteins.
Fabian, H. and Vogel, H.J.
Methods in Molecular Biology 173 : 57-74. 2002

Conditional knockout mice.
Kühn, R. and Schwenk, F.
Methods in Molecular Biology 209 : 159-185. 2002

Cre/loxP recombination system and gene targeting.
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