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Huntingtin fragments form aggresome-like inclusion bodies in mammalian cells

Item Type:Article
Title:Huntingtin fragments form aggresome-like inclusion bodies in mammalian cells
Creators Name:Boeddrich, A., Lurz, R. and Wanker, E.E.
Abstract:The formation of large perinuclear inclusion bodies containing protein aggregates was first described in HeLa cells. Woijcik et al. (1) have shown that treatment of HeLa cells with the proteasome inhibitor PSI [N-benzylo-xycarbonal-Ile-Glu(O-t-butyl)-Ala-leucinal] results in the accumulation of electron-dense material in the vicinity of the Golgi apparatus. These structures were termed proteolysis centers, because ubiquitin as well as components of the 26S proteasome are enriched in inclusion bodies, whereas carbohydrates, lipids, or nucleic acids are not present.
Keywords:Biological Models, Cell Line, Electron Microscopy, Huntington Disease, Inclusion Bodies, Nerve Tissue Proteins, Nuclear Proteins, Protein Folding, Animals, Mammals
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Protein misfolding and disease : principles and Protocols
Publisher:Springer / Humana Press
Page Range:217-229
Official Publication:https://doi.org/10.1385/1-59259-394-1:217
PubMed:View item in PubMed

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