Browse by Research Team

2022

Combinatorial treatment with statins and niclosamide prevents CRC dissemination by unhinging the MACC1-β-catenin-S100A4 axis of metastasis.
Kortüm, B. and Radhakrishnan, H. and Zincke, F. and Sachse, C. and Burock, S. and Keilholz, U. and Dahlmann, M. and Walther, W. and Dittmar, G. and Kobelt, D. and Stein, U.
Oncogene 41 (39): 4446-4458. 23 September 2022

A brief introduction to current cancer gene therapy.
Kobelt, D. and Pahle, J. and Walther, W.
Methods in Molecular Biology 2521 (3): 1-21. 23 June 2022

Capillary gel electrophoresis (CGE) for quality control of plasmid DNA in gene therapy: quality control of 20 years stored GMP-grade plasmid DNA.
Schmeer, M. and Schleef, M. and Shankar, R. and Kobelt, D. and Walther, W.
Methods in Molecular Biology 2521 (3): 317-328. 23 June 2022

Claudin-targeted suicide gene therapy for claudin-overexpressing tumor cells by using modified clostridium perfringens enterotoxin (CPE).
Beier, L.S. and Piontek, J. and Piontek, A. and Protze, J. and Kobelt, D. and Walther, W.
Methods in Molecular Biology 2521 (3): 173-188. 23 June 2022

Gene gun Her2/neu DNA vaccination: evaluation of vaccine efficacy in a syngeneic Her2/neu mouse tumor model.
Nguyen-Hoai, T. and Hohn, O. and Pezzutto, A. and Westermann, J.
Methods in Molecular Biology 2521 (3): 129-154. 23 June 2022

Generation of redirected engineered human chimeric antigen receptor (CAR) T cells.
Bunse, M. and Höpken, U.E.
Methods in Molecular Biology 2521 (3): 67-83. 23 June 2022

TCR gene therapy for cancer.
Rhein, S. and Çakmak-Görür, N.
Methods in Molecular Biology 2521 (3): 95-128. 23 June 2022

2017

In colon epithelia, Clostridium perfringens enterotoxin causes focal leaks by targeting claudins which are apically accessible due to tight junction derangement.
Eichner, M. and Augustin, C. and Fromm, A. and Piontek, A. and Walther, W. and Buecker, R. and Fromm, M. and Krause, G. and Schulzke, J.D. and Guenzel, D. and Piontek, J.
Journal of Infectious Diseases 217 (1): 147-157. 27 December 2017

Statin and rottlerin small-molecule inhibitors restrict colon cancer progression and metastasis via MACC1.
Juneja, M. and Kobelt, D. and Walther, W. and Voss, C. and Smith, J. and Specker, E. and Neuenschwander, M. and Gohlke, B.O. and Dahlmann, M. and Radetzki, S. and Preissner, R. and von Kries, J.P. and Schlag, P.M. and Stein, U.
PLoS Biology 15 (6): e2000784. 1 June 2017

Choosing wisely - Preclinical test models in the era of precision medicine.
Klinghammer, K. and Walther, W. and Hoffmann, J.
Cancer Treatment Reviews 55 : 36-45. April 2017

In vitro and in vivo investigations into the carbene gold chloride and thioglucoside anticancer drug candidates NHC-AuCl and NHC-AuSR.
Walther, W. and Dada, O. and O’Beirne, C. and Ott, I. and Sanchez-Sanz, G. and Schmidt, C. and Werner, C. and Zhu, X. and Tacke, M.
Letters in Drug Design & Discovery 14 (2): 125-134. 2017

2016

SPON2, a newly identified target gene of MACC1, drives colorectal cancer metastasis in mice and is prognostic for colorectal cancer patient survival.
Schmid, F. and Wang, Q. and Huska, M.R. and Andrade-Navarro, M.A. and Lemm, M. and Fichtner, I. and Dahlmann, M. and Kobelt, D. and Walther, W. and Smith, J. and Schlag, P.M. and Stein, U.
Oncogene 35 (46): 5942-5952. 17 November 2016

Vectors and strategies for nonviral cancer gene therapy.
Pahle, J. and Walther, W.
Expert Opinion on Biological Therapy 16 (4): 443-461. April 2016

2015

Chemosensitization by diverging modulation by short-term and long-term TNF-α action on ABCB1 expression and NF-κB signaling in colon cancer.
Walther, W. and Kobelt, D. and Bauer, L. and Aumann, J. and Stein, U.
International Journal of Oncology 47 (6): 2276-2285. December 2015

Oncoleaking: use of the pore-forming clostridium perfringens enterotoxin (CPE) for suicide gene therapy.
Pahle, J. and Aumann, J. and Kobelt, D. and Walther, W.
Methods in Molecular Biology 1317 : 69-85. 2015

2014

In vitro and in vivo investigations into the carbene copper bromide anticancer drug candidate WBC4.
Walther, W. and Fichtner, I. and Hackenberg, F. and Streciwilk, W. and Tacke, M.
Letters in Drug Design & Discovery 11 (7): 825-832. August 2014

Preclinical study on combined chemo- and nonviral gene therapy for sensitization of melanoma using a human TNF-alpha expressing MIDGE DNA vector.
Kobelt, D. and Aumann, J. and Schmidt, M. and Wittig, B. and Fichtner, I. and Behrens, D. and Lemm, M. and Freundt, G. and Schlag, P.M. and Walther, W.
Molecular Oncology 8 (3): 609-619. May 2014

2013

A seven-year storage report of good manufacturing practice-grade naked plasmid DNA: stability, topology, and in vitro/in vivo functional analysis.
Walther, W. and Schmeer, M. and Kobelt, D. and Baier, R. and Harder, A. and Walhorn, V. and Anselmetti, D. and Aumann, J. and Fichtner, I. and Schleef, M.
Human Gene Therapy Clinical Development 24 (4): 147-153. December 2013

Current status of gene therapy for cancer.
Walther, W. and Schlag, P.M.
Current Opinion in Oncology 25 (6): 659-664. November 2013

The activity of titanocene T against xenografted Caki-1 tumors.
Walther, W. and Fichtner, I. and Deally, A. and Hogan, M. and Tacke, M.
Letters in Drug Design & Discovery 10 (5): 375-381. June 2013

Minicircle-based vectors for nonviral gene therapy: in vitro characterization and in vivo application.
Kobelt, D. and Aumann, J. and Schleef, M. and Schmeer, M. and Stein, U. and Schlag, P.M. and Walther, W.
In: Minicircle and miniplasmid DNA vectors: the future of nonviral and viral gene transfer. Wiley, 177-188. ISBN 978-3-527-32456-9 17 April 2013

Performance of high quality minicircle DNA for in vitro and in vivo gene transfer.
Kobelt, D. and Schleef, M. and Schmeer, M. and Aumann, J. and Schlag, P.M. and Walther, W.
Molecular Biotechnology 53 (1): 80-89. January 2013

2012

HER2/neu DNA vaccination by intradermal gene delivery in a mouse tumor model: gene gun is superior to jet injector in inducing CTL responses and protective immunity.
Nguyen-Hoai, T. and Kobelt, D. and Hohn, O. and Vu, M.D. and Schlag, P.M. and Doerken, B. and Norley, S. and Lipp, M. and Walther, W. and Pezzutto, A. and Westermann, J.
OncoImmunology 1 (9): 1537-1545. 1 December 2012

In vivo imaging of colorectal cancer growth and metastasis by targeting MACC1 with shRNA in xenografted mice.
Pichorner, A. and Sack, U. and Kobelt, D. and Kelch, I. and Arlt, F. and Smith, J. and Walther, W. and Schlag, P.M. and Stein, U.
Clinical and Experimental Metastasis 29 (6): 573-583. August 2012

Intratumoral dispersion, retention, systemic biodistribution, and clearance of a small-size tumor necrosis factor-α-expressing MIDGE vector after nonviral in vivo jet-injection gene transfer.
Galling, N. and Kobelt, D. and Aumann, J. and Schmidt, M. and Wittig, B. and Schlag, P.M. and Walther, W.
Human Gene Therapy Part B Methods 23 (4): 264-270. August 2012

Novel Clostridium perfringens enterotoxin suicide gene therapy for selective treatment of claudin-3- and -4-overexpressing tumors.
Walther, W. and Petkov, S. and Kuvardina, O.N. and Aumann, J. and Kobelt, D. and Fichtner, I. and Lemm, M. and Piontek, J. and Blasig, I.E. and Stein, U. and Schlag, P.M.
Gene Therapy 19 (5): 494-503. May 2012

Impact of mutant beta-catenin on ABCB1 expression and therapy response in colon cancer cells.
Stein, U. and Fleuter, C. and Siegel, F. and Smith, J. and Kopacek, A. and Scudiero, D.A. and Hite, K.M. and Schlag, P.M. and Shoemaker, R.H. and Walther, W.
British Journal of Cancer 106 (8): 1395-1405. 10 April 2012

2011

S100A4-induced cell motility and metastasis is restricted by the Wnt/β-catenin pathway inhibitor calcimycin in colon cancer cells.
Sack, U. and Walther, W. and Scudiero, D. and Selby, M. and Aumann, J. and Lemos, C. and Fichtner, I. and Schlag, P.M. and Shoemaker, R.H. and Stein, U.
Molecular Biology of the Cell 22 (18): 3344-3354. September 2011

Novel effect of antihelminthic niclosamide on S100A4-mediated metastatic progression in colon cancer.
Sack, U. and Walther, W. and Scudiero, D. and Selby, M. and Kobelt, D. and Lemm, M. and Fichtner, I. and Schlag, P.M. and Shoemaker, R.H. and Stein, U.
Journal of the National Cancer Institute 103 (13): 1018-1036. 6 July 2011

Intervening in beta-catenin signaling by sulindac inhibits S100A4-dependent colon cancer metastasis.
Stein, U. and Arlt, F. and Smith, J. and Sack, U. and Herrmann, P. and Walther, W. and Lemm, M. and Fichtner, I. and Shoemaker, R.H. and Schlag, P.M.
Neoplasia 13 (2): 131-144. February 2011

2010

Identification of Y-box binding protein 1 as a core regulator of MEK/ERK pathway-dependent gene signatures in colorectal cancer cells.
Juerchott, K. and Kuban, R.J. and Krech, T. and Blüthgen, N. and Stein, U. and Walther, W. and Friese, C. and Kielbasa, S.M. and Ungethuem, U. and Lund, P. and Knoesel, T. and Kemmner, W. and Morkel, M. and Fritzmann, J. and Schlag, P.M. and Birchmeier, W. and Krueger, T. and Sperling, S. and Sers, C. and Royer, H.D. and Herzel, H. and Schaefer, R.
PLoS Genetics 6 (12): e1001231. 2 December 2010

Activation of the CMV-IE promoter by hyperthermia in vitro and in vivo: biphasic heat induction of cytosine deaminase suicide gene expression.
Kobelt, D. and Aumann, J. and Fichtner, I. and Stein, U. and Schlag, P.M. and Walther, W.
Molecular Biotechnology 46 (2): 197-205. October 2010

MACC1 - more than metastasis? Facts and predictions about a novel gene.
Stein, U. and Dahlmann, M. and Walther, W.
Journal of Molecular Medicine 88 (1): 11-18. January 2010

Jet-injection of short hairpin RNA-encoding vectors into tumor cells.
Walther, W. and Stein, U. and Lage, H.
Methods in Molecular Biology 629 : 123-139. 2010

2009

MACC1 controls Met: What a difference an Sp1 site makes.
Stein, U. and Smith, J. and Walther, W. and Arlt, F.
Cell Cycle 8 (15): 2467-2469. 1 August 2009

Heat-responsive gene expression for gene therapy.
Walther, W. and Stein, U.
Advanced Drug Delivery Reviews 61 (7-8): 641-649. 2 July 2009

MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis.
Stein, U. and Walther, W. and Arlt, F. and Schwabe, H. and Smith, J. and Fichtner, I. and Birchmeier, W. and Schlag, P.M.
Nature Medicine 15 (1): 59-67. January 2009

Nonviral jet-injection technology for intratumoral in vivo gene transfer of naked DNA.
Walther, W. and Fichtner, I. and Schlag, P.M. and Stein, U.S.
Methods in Molecular Biology 542 : 195-208. 2009

2008

Novel jet-injection technology for nonviral intratumoral gene transfer in patients with melanoma and breast cancer.
Walther, W. and Siegel, R. and Kobelt, D. and Knoesel, T. and Dietel, M. and Bembenek, A. and Aumann, J. and Schleef, M. and Baier, R. and Stein, U. and Schlag, P.M.
Clinical Cancer Research 14 (22): 7545-7553. 15 November 2008

Complete in vivo reversal of the multidrug resistance phenotype by jet-injection of anti-MDR1 short hairpin RNA-encoding plasmid DNA.
Stein, U. and Walther, W. and Stege, A. and Kaszubiak, A. and Fichtner, I. and Lage, H.
Molecular Therapy 16 (1): 178-186. January 2008

Lokale nicht-virale Gentherapie in kutanen Metastasen von Mammakarzinom und Melanom: Ergebnisse einer Phase-I-Studie mittels Jet-Injektion nackter DNA.
Siegel, R. and Kobelt, D. and Knoesel, T. and Stein, U. and Schlag, P.M. and Walther, W.
Chirurgisches Forum 2008 37 : 131-132. 2008

2007

Heat-inducible in vivo gene therapy of colon carcinoma by human mdr1 promoter-regulated tumor necrosis factor-alpha expression.
Walther, W. and Arlt, F. and Fichtner, I. and Aumann, J. and Stein, U. and Schlag, P.M.
Molecular Cancer Therapeutics 6 (1): 236-243. January 2007

2006

The metastasis-associated gene S100A4 is a novel target of beta-catenin/T-cell factor (TCF) signaling in colon cancer.
Stein, U. and Arlt, F. and Walther, W. and Smith, J. and Waldman, T. and Harris, E.D. and Mertins, S.D. and Heizmann, C.W. and Allard, D. and Birchmeier, W. and Schlag, P.M. and Shoemaker, R.H.
Gastroenterology 131 (5): 1486-1500. November 2006

Reversal of ABC transporter-dependent multidrug resistance in cancer: A realistic option?
Stein, U. and Walther, W.
American Journal of Cancer 5 (5): 285-297. September 2006

Uptake, biodistribution, and time course of naked plasmid DNA trafficking after intratumoral in vivo jet injection.
Walther, W. and Minow, T. and Martin, R. and Fichtner, I. and Schlag, P.M. and Stein, U.
Human Gene Therapy 17 (6): 611-624. 15 June 2006

"Multidrug-Resistenz". Zellulaere Transportprobleme und ihre Konsequenzen ["Multidrug resistance". Cellular transport problems and their consequences].
Stein, U. and Walther, W. and Schlag, P.M.
Paediatrische Praxis 68 : 11-19. 1 January 2006

2005

Nonviral jet-injection gene transfer for efficient in vivo cytosine deaminase suicide gene therapy of colon carcinoma.
Walther, W. and Stein, U. and Fichtner, I. and Kobelt, D. and Aumann, J. and Arlt, F. and Schlag, P.M.
Molecular Therapy 12 (6): 1176-1184. 29 September 2005

Needleless jet injection of naked DNA for nonviral in vivo gene transfer.
Walther, W. and Stein, U.
In: DNA-Pharmaceuticals: Formulation and Delivery in Gene Therapy, DNA Vaccination and Immunotherapy. Wiley-VCH, 133-143. ISBN 9783527311873 25 August 2005

"Multidrug-Resistenz". Zellulaere Transportprobleme und ihre Konsequenzen ["Multidrug resistance". Cellular transport problems and their consequences].
Stein, U. and Walther, W. and Schlag, P.M.
Taegliche Praxis 46 (1): 125-133. 1 January 2005

Use of the nuclease inhibitor aurintricarboxylic acid (ATA) for improved non-viral intratumoral in vivo gene transfer by jet-injection.
Walther, W. and Stein, U. and Siegel, R. and Fichtner, I. and Schlag, P.M.
Journal of Gene Medicine 7 (4): 477-485. 1 January 2005

YB-1 facilitates basal and 5-fluorouracil-inducible expression of the human major vault protein (MVP) gene.
Stein, U. and Bergmann, S. and Scheffer, G.L. and Scheper, R.J. and Royer, H.D. and Schlag, P.M. and Walther, W.
Oncogene 24 (22): 3606-3618. 1 January 2005

2004

Low-volume jet injection for efficient nonviral in vivo gene transfer.
Walther, W. and Stein, U. and Fichtner, I. and Schlag, P.M.
Molecular Biotechnology 28 (2): 121-128. 1 January 2004

Reversal of P-glycoprotein mediated vincristine resistance of L1210/VCR cells by analogues of pentoxifylline - A QSAR study.
Kupsakova, I. and Rybar, A. and Docolomansky, P. and Drobna, Z. and Stein, U. and Walther, W. and Barancik, M. and Breier, A.
European Journal of Pharmaceutical Sciences 21 (2-3): 283-293. 1 January 2004

2003

Stability analysis for long-term storage of naked DNA: impact on nonviral in vivo gene transfer.
Walther, W. and Stein, U. and Voss, C. and Schmidt, T. and Schleef, M. and Schlag, P.M.
Analytical Biochemistry 318 (2): 230-235. 15 July 2003

Process for treating tumors with cytotoxic substances.
Stein, U. and Walther, W. and Schlag, P.M.
US6,582,690. 24 June 2003

Lipoplexes with alkylphospholipid as new helper lipid for efficient in vitro and in vivo gene transfer in tumor therapy.
Zeisig, R. and Ress, A. and Fichtner, I. and Walther, W.
Cancer Gene Therapy 10 (4): 302-311. April 2003

Functional haplotypes of the RET proto-oncogene promoter are associated with Hirschsprung disease (HSCR).
Fitze, G. and Appelt, H. and Koenig, I.R. and Goergens, H. and Stein, U. and Walther, W. and Gossen, M. and Schreiber, M. and Ziegler, A. and Roesner, D. and Schackert, H.K.
Human Molecular Genetics 12 (24): 3207-3214. 1 January 2003

YB-1 as a cell cycle-regulated transcription factor facilitating cyclin A and cyclin B1 gene expression.
Juerchott, K. and Bergmann, S. and Stein, U. and Walther, W. and Janz, M. and Manni, I. and Piaggio, G. and Fietze, E. and Dietel, M. and Royer, H.D.
Journal of Biological Chemistry 278 (30): 27988-27996. 1 January 2003

2002

Intratumoral low-volume jet-injection for efficient nonviral gene transfer.
Walther, W. and Stein, U. and Fichtner, I. and Voss, C. and Schmidt, T. and Schleef, M. and Nellessen, T. and Schlag, P.M.
Molecular Biotechnology 21 (2): 105-115. 1 June 2002

Verwendung des humanen LRP/MVP Promotors fuer einen Therapie-induzierbaren Vektor [Use of the human LRP/MVP promotor of a vector that can be induced by therapy].
Stein, U. and Lange, C. and Walther, W. and Schlag, P.M.
DE10055383A1 ; EP1332219 ; WO0238188. 29 May 2002

Pentoxifylline influences drug transport activity of P-glycoprotein and decreases mdr1 gene expression in multidrug resistant mouse leukemic L1210/VCR cells.
Drobna, Z. and Stein, U. and Walther, W. and Barancik, M. and Breier, A.
General Physiology and Biophysics 21 (1): 103-109. March 2002

Impact of BCRP/MXR, MRP1 and MDR1/P-glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer.
Stein, U. and Lage, H. and Jordan, A. and Walther, W. and Bates, S.E. and Litman, T. and Hohenberger, P. and Dietel, M.
International Journal of Cancer 97 (6): 751-760. 1 January 2002

Tumorgenese.
Schlag, P.M. and Estevez-Schwarz, L. and Kemmner, W. and Schumacher, K. and Walther, W.
In: Chirurgische Onkologie. Thieme, Stuttgart [u.a.], 2-17. ISBN 3-13-126111-0 1 January 2002

Use of the human MDR1 promoter for heat-inducible expression of therapeutic genes.
Walther, W. and Stein, U. and Schlag, P.M.
International Journal of Cancer 98 (2): 291-296. 1 January 2002

Strategien zur Gentherapie von Tumorerkrankungen [Gene therapy strategies for tumor diseases].
Walther, W. and Stein, U. and Schlag, P.M.
Chirurgische Praxis 60 : 19-26. 2002

2001

Hyperthermia-induced nuclear translocation of transcription factor YB-1 leads to enhanced expression of multidrug resistance-related ABC transporters.
Stein, U. and Juerchott, K. and Walther, W. and Bergmann, S. and Schlag, P.M. and Royer, H.D.
Journal of Biological Chemistry 276 (30): 28562-28569. 1 January 2001

In vivo Gentransfer nackter DNA in xenotransplantierte Kolonkarzinome in der Nude Maus mit Hilfe der Jet-Injektion [In vivo gene transfer of naked DNA into xenotransplanted colon carcinoma by jet-injection].
Walther, W. and Stein, U. and Fichtner, I. and Schlag, P.M.
Chirurgisches Forum 30 : 69-72. 1 January 2001

Nonviral in vivo gene delivery into tumors using a novel low volume jet-injection technology.
Walther, W. and Stein, U. and Fichtner, I. and Malcherek, L. and Lemm, M. and Schlag, P.M.
Gene Therapy 8 (3): 173-180. 1 January 2001

2000

Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter.
Lange, C. and Walther, W. and Schwabe, H. and Stein, U.
Biochemical and Biophysical Research Communications 278 (1): 125-133. 11 November 2000

In vivo gene transfer by low-volume jet injection.
Cartier, R. and Ren, S.V. and Walther, W. and Stein, U. and Lewis, A. and Schlag, P.M. and Li, M. and Furth, P.A.
Analytical Biochemistry 282 (2): 262-265. 1 July 2000

Mittel zum nichtviralen Transfer von DNS in eukaryotische Zellen.
Zeisig, R. and Walther, W.
DE19959690A1. 8 June 2000

mdr1 promoter-driven tumor necrosis factor-alpha expression for a chemotherapy-controllable combined in vivo gene therapy and chemotherapy of tumors.
Walther, W. and Stein, U. and Fichtner, I. and Alexander, M. and Shoemaker, R.H. and Schlag, P.M.
Cancer Gene Therapy 7 (6): 893-900. 1 June 2000

High-copy cDNA amplification of minimal total RNA quantities for gene expression analyses.
Schwabe, H. and Stein, U. and Walther, W.
Molecular Biotechnology 14 (2): 165-172. 1 February 2000

Chemotherapy-inducible vector for gene therapy of cancer.
Walther, W. and Stein, U. and Shoemaker, R.H. and Schlag, P.M.
In: Gene Therapy of Cancer : Methods and Protocols ; 35. Methods in Molecular Medicine ; 35 . Humana Press, Totowa, 371-392. 1 January 2000

Gene Therapy of Cancer : Methods and Protocols.
Walther, W. and Stein, U.
Methods in Molecular Medicine ; 35 . Humana Press, Totowa. 1 January 2000

Viral vectors for gene transfer. A review of their use in the treatment of human diseases.
Walther, W. and Stein, U.
Drugs 60 (2): 249-271. 1 January 2000

1999

Therapeutic genes for cancer gene therapy.
Walther, W. and Stein, U.
Molecular Biotechnology 13 (1): 21-28. November 1999

GLI gene expression in bone and soft tissue sarcomas of adult patients correlates with tumor grade.
Stein, U. and Eder, C. and Karsten, U. and Haensch, W. and Walther, W. and Schlag, P.M.
Cancer Research 59 : 1890-1895. 1 January 1999

Hyperthermia for treatment of rectal cancer: evaluation for induction of multidrug resistance gene (mdr1) expression.
Stein, U. and Rau, B. and Wust, P. and Walther, W. and Schlag, P.M.
International Journal of Cancer 80 : 5-12. 1 January 1999

1998

Verwendung von Cytokinen und cytotoxischen Substanzen in einem neuen Verfahren zur Tumorbehandlung [Use of cytokines and cytotoxic substances in a new method to treat tumors].
Stein, U. and Walther, W. and Schlag, P.M.
EP0832653 ; EP0935468 ; WO9811911. 1 April 1998

Cytokine-mediated reversal multidrug resistance.
Stein, U. and Walther, W.
Cytotechnology 27 : 271-282. 1 January 1998

IL-2 gene transfer for chemosensitization of multidrug-resistant human colon carcinoma cells.
Stein, U. and Walther, W. and Shoemaker, R.H. and Schlag, P.M.
1 January 1998

In vivo evaluation of a cytostatic drug-inducible vector for the expression of TNF-alpha.
Walther, W. and Stein, U. and Fichtner, I. and Shoemaker, R.H. and Alexander, M. and Schlag, P.M.
Proceedings of the American Association of Cancer Research 39 : 520. 1 January 1998

In vivo evaluation of a drug-inducible vector system for the combined gene and chemotherapy of cancer.
Walther, W. and Stein, U. and Fichtner, I. and Naundorf, H. and Alexander, M. and Shoemaker, R.H. and Schlag, P.M.
1 January 1998

Large and small scale RNA preparations from eukaryotic cells.
Uckert, W. and Walther, W. and Stein, U.
In: RNA Isolation and Characterization Protocols. Methods in Molecular Biology ; 86 . Humana Press, Totowa,NJ, 7-14. 1 January 1998

Reversal of multidrug resistance by cytokines - evaluation of an alternative therapeutic approach in human tumors.
Stein, U. and Walther, W. and Rau, B. and Kettelhack, C. and Schlag, P.M.
Proceedings of the American Association of Cancer Research 39 : 509. 1 January 1998

1997

Development and characterisation of novel human multidrug resistant mammary carcinoma lines in vitro and in vivo.
Stein, U. and Walther, W. and Lemm, M. and Naundorf, H. and Fichtner, I.
International Journal of Cancer 72 : 885-891. 1 January 1997

Development and characterization of novel multidrug resistant breast cancer lines.
Walther, W. and Stein, U. and Lemm, M. and Naundorf, H. and Fichtner, I.
Proceedings of the American Association of Cancer Research 38 : 484. 1 January 1997

Diverse effects of tumor necrosis factor a on expression of the multidrug resistance-associated genes LRP and MRP.
Stein, U. and Walther, W. and Laurencot, C.M. and Scheffer, G.L. and Scheper, R.J. and Shoemaker, R.H.
Proceedings of the American Association of Cancer Research 38 : 480. 1 January 1997

Employment of the mdr1 promoter for the chemotherapy-inducible expression of therapeutic genes in cancer gene therapy.
Walther, W. and Wendt, J. and Stein, U.
Gene Therapy 4 : 544-552. 1 January 1997

In situ RT-PCR using fluorescence-labeled primers.
Stein, U. and Walther, W. and Wendt, J. and Schild, T.A.
Biotechniques 23 : 194-198. 1 January 1997

Modulation of MDR1 expression by cytokines in human colon carcinoma cells: an approach for reversal of multidrug.
Stein, U. and Walther, W. and Shoemaker, R.H.
Biotherapy in Cancer 1 : 53-53. 1 January 1997

Tumor necrosis factor-alpha and expression of the multidrug resistance-associated genes LRP and MRP.
Stein, U. and Walther, W. and Laurencot, C.M. and Scheffer, G.L. and Scheper, R.J. and Shoemaker, R.H.
Journal of the National Cancer Institute 89 : 807-813. 1 January 1997

1996

Cell type specific and inducible promoters for vectors in gene therapy as an approach for cell targeting.
Walther, W. and Stein, U.
Journal of Molecular Medicine 74 : 379-392. 1 January 1996

Drug-inducible vector for the conditional expression of the human TNFalpha gene.
Walther, W. and Stein, U. and Wendt, J. and Shoemaker, R.H.
Proceedings of the American Association of Cancer Research 37 : 350. 1 January 1996

Modulation of mdr1 expression by cytokines in human colon carcinoma cells: an approach for reversal of multidrug resistance.
Stein, U. and Walther, W. and Shoemaker, H.
British Journal of Cancer 74 : 1384-1391. 1 January 1996

Reversal of multidrug resistance by transduction of cytokine genes into human colon carcinoma cells.
Stein, U. and Walther, W. and Shoemaker, R.H.
Journal of the National Cancer Institute 88 : 1383-1392. 1 January 1996

Targeted vectors for gene therapy of cancer and retroviral infections.
Walther, W. and Stein, U.
Molecular Biotechnology 6 : 267-286. 1 January 1996

Vincristine induction of mutant and wild-type human multidrug-resitance promoters is cell- type-specific and dose-dependent.
Stein, U. and Walther, W. and Shoemaker, R.H.
Journal of Cancer Research and Clinical Oncology 122 : 275-282. 1 January 1996

1995

Gene transfer of human TNFalpha into glioblastoma cells permits modulation of mdr1 expression and potentiation of chemosensitivity.
Walther, W. and Stein, U. and Pfeil, D.
International Journal of Cancer 61 (6): 832-839. 1 January 1995

Influence of retrovirally transduced human tumor-necrosis-factor-alpha on the expression of C-myc, k-ras, C-jun, p53, tgf-alpha, and cea in human colon-carcinoma cell-lines.
Uckert, W. and Walther, W. and Hummel, O.
International Journal of Oncology 6 : 1027-1031. 1 January 1995

Liposome mediated transfer of marker and cytokine genes into rat and human glioblastoma cells in vitro and in vivo.
Reszka, R. and Zhu, J. and Weber, F. and Walther, W. and Greferath, R. and Dyballa, S.
Journal of Liposome Research 5 : 149-167. 1 January 1995

1994

Vektor zur Expression von therapeutisch relevanten Genen [Vector for the expression of therapy-relevant genes].
Stein, U. and Walther, W.
DE4238778A1 ; DE4238778C2 ; EP0670904 ; US 5,968,735. 19 May 1994

Conditional expression of human TNF-alpha: a system for inducible cytotoxicity.
Sparmann, G. and Walther, W. and Guenzburg, W.H. and Uckert, W. and Salmons, B.
International Journal of Cancer 59 : 103-107. 1 January 1994

Detektion des P-Glycoproteins mit dem Antikoerper Anti-P-Glycoprotein 170-180 (Klon JSB-1): Modulation der Multidrug Resistenz in Zytokin-Gen-transfizierten Glioblastomzellen.
Stein, U. and Walther, W.
Boehringer Mannheim Biochemica Information 92 : 22-23. 1 January 1994

Influence of cytokines on mdr1 expression in human colon carcinoma cell lines: increased cytotoxicity of MDR relevant drugs.
Walther, W. and Stein, U.
Journal of Cancer Research and Clinical Oncology 120 : 471-478. 1 January 1994

RNA analysis using miniprep RNA in reverse transcriptase PCR.
Walther, W. and Stein, U. and Eder, C.
Biotechniques 17 : 674-675. 1 January 1994

Retrovirus-mediated gene transfer in cancer therapy.
Uckert, W. and Walther, W.
Pharmacology & Therapeutics 63 : 323-347. 1 January 1994

Point mutations in the mdr1 promoter of human osteosarcomas are associated with in vitro responsiveness to multidrug resistance relevated drugs.
Stein, U. and Walther, W. and Wunderlich, V.
European Journal of Cancer 30A (10): 1541-1545. 1994

1993

Rapid method of total RNA mini-preparation from eucaryotic cells.
Walther, W. and Stein, U. and Uckert, W.
Nucleic Acids Research 21 (7): 1682. 11 April 1993

Retrovirus-mediated gene transfer of tumor necrosis factor alpha into colon carcinoma cells generates a growth inhibition.
Walther, W. and Fichtner, I. and Uckert, W.
Anticancer Research 13 : 1565-1574. 1 January 1993

1992

Retroviral transduction of the human TNF alpha gene into tumour cells generates a growth inhibitory effect.
Walther, W. and Huth, J. and Sparmann, G. and Uckert, W.
In: European Biotechnology Today. INTERCEPT Ltd., Andover, 209-216. 1 January 1992