2025

Unlocking antigen flexibility with widened patient access: CD3FR in off-the-shelf CAR T cells.
Novikova, Nataliia and Huppa, Johannes B. ORCID: https://orcid.org/0000-0003-2634-8198
Molecular Therapy 33 (9): 4029-4031. 3 September 2025

CD4+T-cells create a stable mechanical environment for force-sensitive TCR:pMHC interactions.
Schrangl, Lukas ORCID: https://orcid.org/0000-0002-0420-7872, Kellner, Florian, Platzer, René ORCID: https://orcid.org/0009-0001-3399-4721, Mühlgrabner, Vanessa, Hubinger, Paul, Wieland, Josephine, Obst, Reinhard, Toca-Herrera, José L. ORCID: https://orcid.org/0000-0001-8951-2616, Huppa, Johannes B. ORCID: https://orcid.org/0000-0003-2634-8198, Schütz, Gerhard J. ORCID: https://orcid.org/0000-0003-1542-1089 and Göhring, Janett
Nature Communications 16 (1): 7577. 15 August 2025

Gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice.
Mühlgrabner, V., Plach, A., Holler, J., Leitner, J., Steinberger, P., Dupré, L., Göhring, J. and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Methods in Cell Biology 193 : 127-154. 5 February 2025

2024

TCR/CD3-based synthetic antigen receptors (TCC) convey superior antigen sensitivity combined with high fidelity of activation.
Mühlgrabner, V., Peters, T., Velasco Cárdenas, R.M.H., Salzer, B., Göhring, J., Plach, A., Höhrhan, M., Perez, I.D., Goncalves, V.D.R., Farfán, J.S., Lehner, M., Stockinger, H., Schamel, W.W., Schober, K., Busch, D.H., Hudecek, M., Dushek, O., Minguet, S., Platzer, R. and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Science Advances 10 (36): eadj4632. 6 September 2024

Advanced quantification of receptor-ligand interaction lifetimes via single-molecule FRET microscopy.
Schrangl, L. ORCID: https://orcid.org/0000-0002-0420-7872, Mühlgrabner, V. ORCID: https://orcid.org/0009-0004-3331-8741, Platzer, R. ORCID: https://orcid.org/0009-0001-3399-4721, Kellner, F. ORCID: https://orcid.org/0000-0001-5931-3798, Wieland, J., Obst, R. ORCID: https://orcid.org/0000-0002-4839-207X, Toca-Herrera, J.L. ORCID: https://orcid.org/0000-0001-8951-2616, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089 and Göhring, J. ORCID: https://orcid.org/0000-0002-6755-5305
Biomolecules 14 (8): 1001. 13 August 2024

CXCR4 has a dual role in improving the efficacy of BCMA redirected CAR-NK cells in multiple myeloma.
Moles, M.W., Erdlei, H., Menzel, L. ORCID: https://orcid.org/0000-0002-3816-6255, Massaro, M., Fiori, A. ORCID: https://orcid.org/0000-0003-0440-4857, Bunse, M. ORCID: https://orcid.org/0000-0002-7554-2520, Schrimpf, M., Gerlach, K., Gudipati, V., Reiser, J., Mathavan, K., Goodrich, J., Huppa, J.B., Kroenke, J., Valamehr, B., Höpken, U.E. ORCID: https://orcid.org/0000-0002-0776-4893 and Rehm, A. ORCID: https://orcid.org/0000-0001-7490-1950
Frontiers in Immunology 15 : 1383136. 24 June 2024

CD3 aptamers promote expansion and persistence of tumor-reactive T cells for adoptive T cell therapy in cancer.
Menon, A.P., Villanueva, H., Meraviglia-Crivelli, D., van Santen, H.M., Hellmeier, J., Zheleva, A., Nonateli, F., Peters, T., Wachsmann, T.L.A., Hernandez-Rueda, M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Schütz, G.J., Sevcsik, E., Moreno, B. and Pastor, F.
Molecular Therapy Nucleic Acids 35 (2): 102198. 11 June 2024

Breakthrough infections in SARS-CoV-2-vaccinated multiple myeloma patients improve cross-protection against omicron variants.
Wagner, A., Garner-Spitzer, E. ORCID: https://orcid.org/0000-0002-5283-0458, Auer, C., Gattinger, P. ORCID: https://orcid.org/0000-0001-6724-8543, Zwazl, I., Platzer, R., Orola-Taus, M., Pichler, P. ORCID: https://orcid.org/0000-0001-8340-0197, Amman, F., Bergthaler, A. ORCID: https://orcid.org/0000-0003-0597-1976, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Zielinski, C.C. ORCID: https://orcid.org/0000-0002-1440-9013, Valenta, R., Kundi, M. ORCID: https://orcid.org/0000-0002-2707-3213 and Wiedermann, U. ORCID: https://orcid.org/0000-0002-1302-3223
Vaccines 12 (5): 518. 9 May 2024

Measurement of forces acting on single T-cell receptors.
Schrangl, L., Göhring, J. ORCID: https://orcid.org/0000-0002-6755-5305, Kellner, F. ORCID: https://orcid.org/0000-0001-5931-3798, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089
Methods in Molecular Biology 2800 : 147-165. 7 May 2024

CAR affinity modulates the sensitivity of CAR-T cells to PD-1/PD-L1-mediated inhibition.
Andreu-Saumell, I. ORCID: https://orcid.org/0009-0006-8897-0846, Rodriguez-Garcia, A. ORCID: https://orcid.org/0000-0003-0906-1793, Mühlgrabner, V., Gimenez-Alejandre, M., Marzal, B., Castellsagué, J., Brasó-Maristany, F. ORCID: https://orcid.org/0000-0001-5440-9643, Calderon, H. ORCID: https://orcid.org/0000-0002-8902-4302, Angelats, L., Colell, S. ORCID: https://orcid.org/0000-0002-2132-2856, Nuding, M., Soria-Castellano, M. ORCID: https://orcid.org/0000-0002-0746-417X, Barbao, P. ORCID: https://orcid.org/0000-0001-7139-4420, Prat, A. ORCID: https://orcid.org/0000-0003-2377-540X, Urbano-Ispizua, A., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Guedan, S. ORCID: https://orcid.org/0000-0002-9605-0760
Nature Communications 15 (1): 3552. 26 April 2024

CAR-mediated targeting of NK cells overcomes tumor immune escape caused by ICAM-1 downregulation.
Eitler, J. ORCID: https://orcid.org/0000-0002-3175-2438, Rackwitz, W., Wotschel, N., Gudipati, V., Murali Shankar, N., Sidorenkova, A., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Ortiz-Montero, P., Opitz, C., Künzel, S.R., Michen, S., Temme, A., Loureiro, L.R., Feldmann, A., Bachmann, M., Boissel, L., Klingemann, H., Wels, W.S. ORCID: https://orcid.org/0000-0001-9858-3643 and Tonn, T. ORCID: https://orcid.org/0000-0001-9580-2193
Journal for ImmunoTherapy of Cancer 12 (2): e008155. 27 February 2024

2023

Harnessing CD3 diversity to optimize CAR T cells.
Velasco Cárdenas, R.M.H., Brandl, S.M. ORCID: https://orcid.org/0000-0002-1632-0127, Meléndez, A.V., Schlaak, A.E., Buschky, A., Peters, T., Beier, F., Serrels, B., Taromi, S. ORCID: https://orcid.org/0000-0001-5657-6568, Raute, K., Hauri, S. ORCID: https://orcid.org/0000-0002-7249-2011, Gstaiger, M., Lassmann, S., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Boerries, M. ORCID: https://orcid.org/0000-0002-3670-0602, Andrieux, G., Bengsch, B. ORCID: https://orcid.org/0000-0003-2552-740X, Schamel, W.W. ORCID: https://orcid.org/0000-0003-4496-3100 and Minguet, S. ORCID: https://orcid.org/0000-0001-8211-5538
Nature Immunology 24 (12): 2135-2149. December 2023

Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.
Platzer, R., Hellmeier, J. ORCID: https://orcid.org/0000-0002-7153-9121, Göhring, J. ORCID: https://orcid.org/0000-0002-6755-5305, Perez, I.D., Schatzlmaier, P., Bodner, C. ORCID: https://orcid.org/0000-0001-5635-4842, Focke-Tejkl, M. ORCID: https://orcid.org/0000-0003-3433-5071, Schütz, G.J., Sevcsik, E., Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Brameshuber, M. ORCID: https://orcid.org/0000-0001-8672-2255 and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
EMBO Reports 24 (11): e57842. 6 November 2023

Lower magnitude and faster waning of antibody responses to SARS-CoV-2 vaccination in anti-TNF-α-treated IBD patients are linked to lack of activation and expansion of cTfh1 cells and impaired B memory cell formation.
Garner-Spitzer, E. ORCID: https://orcid.org/0000-0002-5283-0458, Wagner, A., Gudipati, V., Schoetta, A.M., Orola-Taus, M., Kundi, M., Kunert, R., Mayrhofer, P., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Stockinger, H., Carsetti, R., Gattinger, P., Valenta, R., Kratzer, B. ORCID: https://orcid.org/0000-0003-1091-4327, Sehgal, A.N.A., Pickl, W.F., Reinisch, W., Novacek, G. and Wiedermann, U. ORCID: https://orcid.org/0000-0002-1302-3223
EBioMedicine 96 : 104788. October 2023

Systemic inflammation and normocytic anemia in DOCK11 deficiency.
Block, J., Rashkova, C., Castanon, I., Zoghi, S., Platon, J., Ardy, R.C., Fujiwara, M., Chaves, B., Schoppmeyer, R., van der Made, C.I., Jimenez Heredia, R., Harms, F.L., Alavi, S., Alsina, L., Sanchez Moreno, P., Ávila Polo, R., Cabrera-Pérez, R., Kostel Bal, S., Pfajfer, L., Ransmayr, B., Mautner, A.K., Kondo, R., Tinnacher, A., Caldera, M., Schuster, M., Domínguez Conde, C., Platzer, R., Salzer, E., Boyer, T., Brunner, H.G., Nooitgedagt-Frons, J.E., Iglesias, E., Deyà-Martinez, A., Camacho-Lovillo, M., Menche, J., Bock, C., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Pickl, W.F., Distel, M., Yoder, J.A., Traver, D., Engelhardt, K.R., Linden, T., Kager, L., Hannich, J.T., Hoischen, A., Hambleton, S., Illsinger, S., Da Costa, L., Kutsche, K., Chavoshzadeh, Z., van Buul, J.D., Antón, J., Calzada-Hernández, J., Neth, O., Viaud, J., Nishikimi, A., Dupré, L. and Boztug, K.
New England Journal of Medicine 389 (6): 527-539. 10 August 2023

A DNA origami-based biointerface to interrogate the spatial requirements for sensitized T-Cell antigen recognition.
Hellmeier, J., Platzer, R., Huppa, J.B. and Sevcsik, E.
Methods in Molecular Biology 2654 : 277-302. 28 April 2023

Linoleic acid potentiates CD8(+) T cell metabolic fitness and antitumor immunity.
Nava Lauson, C.B., Tiberti, S., Corsetto, P.A., Conte, F., Tyagi, P., Machwirth, M., Ebert, S., Loffreda, A., Scheller, L., Sheta, D., Mokhtari, Z., Peters, T., Raman, A.T., Greco, F., Rizzo, A.M., Beilhack, A., Signore, G., Tumino, N., Vacca, P., McDonnell, L.A., Raimondi, A., Greenberg, P.D., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Cardaci, S., Caruana, I., Rodighiero, S., Nezi, L. and Manzo, T.
Cell Metabolism 35 (4): 633-650.e9. 4 April 2023

T-cell antigen recognition: catch-as-catch-can or catch-22?
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Schütz, G.J.
EMBO Journal 42 (7): e113507. 3 April 2023

Unique roles of co-receptor-bound LCK in helper and cytotoxic T cells.
Horkova, V. ORCID: https://orcid.org/0000-0002-3679-2236, Drobek, A. ORCID: https://orcid.org/0000-0003-1066-9413, Paprckova, D. ORCID: https://orcid.org/0000-0002-0151-4797, Niederlova, V. ORCID: https://orcid.org/0000-0001-8768-9039, Prasai, A., Uleri, V. ORCID: https://orcid.org/0000-0001-5040-5995, Glatzova, D., Kraller, M., Cesnekova, M., Janusova, S., Salyova, E., Tsyklauri, O. ORCID: https://orcid.org/0000-0001-9997-5913, Kadlecek, T.A., Krizova, K., Platzer, R., Schober, K., Busch, D.H. ORCID: https://orcid.org/0000-0001-8713-093X, Weiss, A., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Stepanek, O. ORCID: https://orcid.org/0000-0002-2735-3311
Nature Immunology 24 (1): 174-185. January 2023

Mechanistic insights into T-Cell antigen recognition through single molecule microscopy.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Schütz, G.J.
In: Encyclopedia of Cell Biology. Encyclopedia of Cell Biology, 5 . Elsevier, 536-552. ISBN 978-0-12-821624-8 2023

2022

EBAG9-silencing exerts an immune checkpoint function without aggravating adverse effects.
Wirges, A. ORCID: https://orcid.org/0000-0001-7038-4503, Bunse, M. ORCID: https://orcid.org/0000-0002-7554-2520, Joedicke, J.J. ORCID: https://orcid.org/0000-0002-4708-3216, Blanc, E. ORCID: https://orcid.org/0000-0002-4369-0254, Gudipati, V., Moles, M.W., Shiku, H., Beule, D. ORCID: https://orcid.org/0000-0002-3284-0632, Huppa, J.B., Höpken, U.E. ORCID: https://orcid.org/0000-0002-0776-4893 and Rehm, A. ORCID: https://orcid.org/0000-0001-7490-1950
Molecular Therapy 30 (11): 3358-3378. 2 November 2022

Mechanosurveillance: Tiptoeing T cells.
Göhring, J., Schrangl, L., Schütz, G.J. and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Frontiers in Immunology 13 : 886328. 26 May 2022

Time 2EVOLVE: predicting efficacy of engineered T-cells - how far is the bench from the bedside?
Guedan, S., Luu, M., Ammar, D., Barbao, P., Bonini, C., Bousso, P., Buchholz, C.J., Casucci, M., De Angelis, B. ORCID: https://orcid.org/0000-0002-7938-737X, Donnadieu, E. ORCID: https://orcid.org/0000-0002-4985-7254, Espie, D., Greco, B., Groen, R., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Kantari-Mimoun, C., Laugel, B., Mantock, M., Markman, J.L., Morris, E., Quintarelli, C. ORCID: https://orcid.org/0000-0002-4343-9705, Rade, M., Reiche, K., Rodriguez-Garcia, A., Rodriguez-Madoz, J.R., Ruggiero, E., Themeli, M., Hudecek, M. and Marchiq, I.
Journal for ImmunoTherapy of Cancer 10 (5): e003487. May 2022

SARS-CoV-2-specific antibody (Ab) levels and the kinetic of ab decline determine Ab persistence over 1 year.
Garner-Spitzer, E., Wagner, A., Kundi, M., Stockinger, H., Ohradanova-Repic, A., Gebetsberger, L., Schoetta, A.M., Gudipati, V., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Kunert, R., Mayrhofer, P., Kreil, T.R., Farcet, M.R., Hoeltl, E. and Wiedermann, U.
Frontiers in Medicine 9 : 822316. 15 February 2022

The frequency of differentiated CD3(+)CD27(-)CD28(-) T cells predicts response to CART cell therapy in diffuse large B-cell lymphoma.
Worel, N., Grabmeier-Pfistershammer, K., Kratzer, B., Schlager, M., Tanzmann, A., Rottal, A., Körmöczi, U., Porpaczy, E., Staber, P.B., Skrabs, C., Herkner, H., Gudipati, V., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Salzer, B., Lehner, M., Saxenhuber, N., Friedberg, E., Wohlfarth, P., Hopfinger, G., Rabitsch, W., Simonitsch-Klupp, I., Jäger, U. and Pickl, W.F.
Frontiers in Immunology 13 : 1004703. 9 January 2022

2021

Automated two-dimensional spatiotemporal analysis of mobile single-molecule FRET probes.
Schrangl, L., Göhring, J., Kellner, F., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Schütz, G.J.
Journal of Visualized Experiments 177 : e63124. 23 November 2021

Three-dimensional single molecule localization microscopy reveals the topography of the immunological synapse at isotropic precision below 15 nm.
Velas, L., Brameshuber, M. ORCID: https://orcid.org/0000-0001-8672-2255, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Kurz, E., Dustin, M.L. ORCID: https://orcid.org/0000-0003-4983-6389, Zelger, P., Jesacher, A. and Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089
Nano Letters 21 (21): 9247-9255. 10 November 2021

Prospective tracking of donor-reactive T-cell clones in the circulation and rejecting human kidney allografts.
Aschauer, C., Jelencsics, K., Hu, K., Heinzel, A., Gregorich, M.G., Vetter, J., Schaller, S., Winkler, S.M., Weinberger, J., Pimenov, L., Gualdoni, G.A., Eder, M., Kainz, A., Troescher, A.R., Regele, H., Reindl-Schwaighofer, R., Wekerle, T., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Sykes, M. and Oberbauer, R.
Frontiers in Immunology 12 : 750005. 14 October 2021

Strategies for the site-specific decoration of DNA origami nanostructures with functionally intact proteins.
Hellmeier, J., Platzer, R., Mühlgrabner, V., Schneider, M.C., Kurz, E., Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Sevcsik, E. ORCID: https://orcid.org/0000-0002-2155-1675
ACS Nano 15 (9): 15057-15068. 28 September 2021

Characterization of CD8 T cell-mediated mutations in the immunodominant epitope GP33-41 of lymphocytic choriomeningitis virus.
Smyth, M., Khamina, K., Popa, A., Gudipati, V., Agerer, B., Lercher, A., Kosack, L., Endler, L., Baazim, H., Viczenczova, C., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Bergthaler, A.
Frontiers in Immunology 12 : 638485. 14 June 2021

Temporal analysis of T-cell receptor-imposed forces via quantitative single molecule FRET measurements.
Göhring, J. ORCID: https://orcid.org/0000-0002-6755-5305, Kellner, F. ORCID: https://orcid.org/0000-0001-5931-3798, Schrangl, L. ORCID: https://orcid.org/0000-0002-0420-7872, Platzer, R., Klotzsch, E., Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089
Nature Communications 12 (1): 2502. 4 May 2021

Histone deacetylase 1 controls CD4(+) T cell trafficking in autoinflammatory diseases.
Hamminger, P., Marchetti, L., Preglej, T., Platzer, R., Zhu, C., Kamnev, A., Rica, R., Stolz, V., Sandner, L., Alteneder, M., Kaba, E., Waltenberger, D., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Trauner, M., Bock, C., Lyck, R., Bauer, J., Dupré, L., Seiser, C., Boucheron, N., Engelhardt, B. and Ellmeier, W.
Journal of Autoimmunity 119 : 102610. May 2021

Adjuvants and vaccines used in allergen-specific immunotherapy induce neutrophil extracellular traps.
Karacs, J., Reithofer, M. ORCID: https://orcid.org/0000-0003-2845-1826, Kitzmüller, C. ORCID: https://orcid.org/0000-0002-3829-8020, Kraller, M., Schmalz, S. ORCID: https://orcid.org/0000-0003-4841-4942, Bleichert, S. ORCID: https://orcid.org/0000-0002-3054-1751, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Bohle, B. and Jahn-Schmid, B. ORCID: https://orcid.org/0000-0002-7979-6667
Vaccines 9 (4): 321. 1 April 2021

SARS-CoV-2 mutations in MHC-I-restricted epitopes evade CD8(+) T cell responses.
Agerer, B., Koblischke, M., Gudipati, V., Montaño-Gutierrez, L.F., Smyth, M., Popa, A., Genger, J.W., Endler, L., Florian, D.M., Mühlgrabner, V., Graninger, M., Aberle, S.W., Husa, A.M., Shaw, L.E., Lercher, A., Gattinger, P., Torralba-Gombau, R., Trapin, D., Penz, T., Barreca, D., Fae, I., Wenda, S., Traugott, M., Walder, G., Pickl, W.F., Thiel, V., Allerberger, F., Stockinger, H., Puchhammer-Stöckl, E., Weninger, W., Fischer, G., Hoepler, W., Pawelka, E., Zoufaly, A., Valenta, R., Bock, C., Paster, W., Geyeregger, R., Farlik, M., Halbritter, F., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Aberle, J.H. and Bergthaler, A.
Science Immunology 6 (57): eabg6461. March 2021

A multimodal platform for simultaneous T-cell imaging, defined activation, and mechanobiological characterization.
Fölser, M., Motsch, V. ORCID: https://orcid.org/0000-0002-4860-4247, Platzer, R., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089
Cells 10 (2): 235. February 2021

DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens.
Hellmeier, J. ORCID: https://orcid.org/0000-0002-7153-9121, Platzer, R., Eklund, A.S. ORCID: https://orcid.org/0000-0003-1047-2100, Schlichthaerle, T. ORCID: https://orcid.org/0000-0003-4444-2575, Karner, A., Motsch, V. ORCID: https://orcid.org/0000-0002-4860-4247, Schneider, M.C. ORCID: https://orcid.org/0000-0002-3771-8353, Kurz, E., Bamieh, V., Brameshuber, M. ORCID: https://orcid.org/0000-0001-8672-2255, Preiner, J. ORCID: https://orcid.org/0000-0002-6755-6543, Jungmann, R. ORCID: https://orcid.org/0000-0003-4607-3312, Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Sevcsik, E. ORCID: https://orcid.org/0000-0002-2155-1675
Proceedings of the National Academy of Sciences of the United States of America 118 (4): e2016857118. 26 January 2021

Functionalized bead assay to measure three-dimensional traction forces during T-cell activation.
Aramesh, M. ORCID: https://orcid.org/0000-0003-2071-1929, Mergenthal, S., Issler, M., Plochberger, B. ORCID: https://orcid.org/0000-0003-2733-9947, Weber, F., Qin, X.H., Liska, R. ORCID: https://orcid.org/0000-0001-7865-1936, Duda, G.N., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Ries, J., Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089 and Klotzsch, E. ORCID: https://orcid.org/0000-0002-7577-9042
Nano Letters 21 (1): 507-514. 13 January 2021

The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-mediated immune responses.
Jongsma, M.L.M., de Waard, A.A., Raaben, M., Zhang, T., Cabukusta, B., Platzer, R., Blomen, V.A., Xagara, A., Verkerk, T., Bliss, S., Kong, X., Gerke, C., Janssen, L., Stickel, E., Holst, S., Plomp, R., Mulder, A., Ferrone, S., Claas, F.H.J., Heemskerk, M.H.M, Griffioen, M., Halenius, A., Overkleeft, H., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Wuhrer, M., Brummelkamp, T.R., Neefjes, J. and Spaapen, R.M.
Immunity 54 (1): 132-150.e9. 12 January 2021

2020

Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy.
Platzer, R., Rossboth, B.K., Schneider, M.C. ORCID: https://orcid.org/0000-0002-3771-8353, Sevcsik, E., Baumgart, F., Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Brameshuber, M. ORCID: https://orcid.org/0000-0001-8672-2255
Nature Communications 11 (1): 4993. 5 October 2020

Engineering AvidCARs for combinatorial antigen recognition and reversible control of CAR function.
Salzer, B. ORCID: https://orcid.org/0000-0003-4282-6356, Schueller, C.M. ORCID: https://orcid.org/0000-0001-7618-6815, Zajc, C.U. ORCID: https://orcid.org/0000-0002-9356-7430, Peters, T., Schoeber, M.A. ORCID: https://orcid.org/0000-0003-4918-5061, Kovacic, B. ORCID: https://orcid.org/0000-0001-7261-0201, Buri, M.C., Lobner, E. ORCID: https://orcid.org/0000-0002-5234-5524, Dushek, O. ORCID: https://orcid.org/0000-0001-5847-5226, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Obinger, C. ORCID: https://orcid.org/0000-0002-7133-3430, Putz, E.M. ORCID: https://orcid.org/0000-0002-9990-4477, Holter, W., Traxlmayr, M.W. ORCID: https://orcid.org/0000-0002-2108-582X and Lehner, M. ORCID: https://orcid.org/0000-0002-5776-4218
Nature Communications 11 (1): 4166. 20 August 2020

Inefficient CAR-proximal signaling blunts antigen sensitivity.
Gudipati, V. ORCID: https://orcid.org/0000-0001-6647-0708, Rydzek, J., Doel-Perez, I. ORCID: https://orcid.org/0000-0003-0443-9718, Gonçalves, V.D.R. ORCID: https://orcid.org/0000-0001-9982-3346, Scharf, L. ORCID: https://orcid.org/0000-0002-1596-2208, Königsberger, S., Lobner, E. ORCID: https://orcid.org/0000-0002-5234-5524, Kunert, R. ORCID: https://orcid.org/0000-0002-3397-3621, Einsele, H., Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Hudecek, M. ORCID: https://orcid.org/0000-0002-2280-2202 and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Nature Immunology 21 (8): 848-856. August 2020

TIM-3 and CEACAM1 do not interact in cis and in trans.
De Sousa Linhares, A., Kellner, F. ORCID: https://orcid.org/0000-0001-5931-3798, Jutz, S., Zlabinger, G.J. ORCID: https://orcid.org/0000-0002-7478-4173, Gabius, H.J., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Leitner, J. ORCID: https://orcid.org/0000-0002-7156-1759 and Steinberger, P. ORCID: https://orcid.org/0000-0001-6848-4097
European Journal of Immunology 50 (8): 1126-1141. August 2020

The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity.
Salzer, E., Zoghi, S., Kiss, M.G., Kage, F., Rashkova, C., Stahnke, S., Haimel, M., Platzer, R., Caldera, M., Ardy, R.C., Hoeger, B., Block, J., Medgyesi, D., Sin, C., Shahkarami, S., Kain, R., Ziaee, V., Hammerl, P., Bock, C., Menche, J., Dupré, L., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Sixt, M., Lomakin, A., Rottner, K., Binder, C.J., Stradal, T.E.B., Rezaei, N. and Boztug, K.
Science Immunology 5 (49): eabc3979. 10 July 2020

Getting CD19 into shape: expression of natively folded "difficult-to-express" CD19 for staining and stimulation of CAR-T cells.
Lobner, E., Wachernig, A., Gudipati, V., Mayrhofer, P., Salzer, B., Lehner, M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Kunert, R.
Frontiers in Bioengineering and Biotechnology 8 : 49. 7 February 2020

2019

Homo- and heteroassociations drive activation of ErbB3.
Váradi, T., Schneider, M., Sevcsik, E., Kiesenhofer, D., Baumgart, F., Batta, G., Kovács, T., Platzer, R., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Szöllősi, J., Schütz, G.J., Brameshuber, M. ORCID: https://orcid.org/0000-0001-8672-2255 and Nagy, P. ORCID: https://orcid.org/0000-0002-7466-805X
Biophysical Journal 117 (10): 1935-1947. 19 November 2019

How drag sharpens a T cell's view on antigen.
Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089 and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Proceedings of the National Academy of Sciences of the United States of America 116 (34): 16669-16671. 20 August 2019

2018

Mutations affecting the actin regulator WD repeat-containing protein 1 lead to aberrant lymphoid immunity.
Pfajfer, L., Mair, N.K, Jiménez-Heredia, R., Genel, F., Gulez, N., Ardeniz, Ö., Hoeger, B., Bal, S.K., Madritsch, C., Kalinichenko, A., Chandra Ardy, R., Gerçeker, B., Rey-Barroso, J., Ijspeert, H., Tangye, S.G., Simonitsch-Klupp, I., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, van der Burg, M., Dupré, L. and Boztug, K.
Journal of Allergy and Clinical Immunology 142 (5): 1589-1604.e11. November 2018

TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells.
Rossboth, B. ORCID: https://orcid.org/0000-0002-9002-9434, Arnold, A.M., Ta, H., Platzer, R., Kellner, F. ORCID: https://orcid.org/0000-0001-5931-3798, Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Brameshuber, M. ORCID: https://orcid.org/0000-0001-8672-2255, Baumgart, F. ORCID: https://orcid.org/0000-0003-1352-8632 and Schütz, G.J. ORCID: https://orcid.org/0000-0003-1542-1089
Nature Immunology 19 (8): 821-827. August 2018

Monomeric TCRs drive T cell antigen recognition.
Brameshuber, M. ORCID: https://orcid.org/0000-0001-8672-2255, Kellner, F. ORCID: https://orcid.org/0000-0001-5931-3798, Rossboth, B.K. ORCID: https://orcid.org/0000-0002-9002-9434, Ta, H., Alge, K., Sevcsik, E., Göhring, J., Axmann, M., Baumgart, F., Gascoigne, N.R.J. ORCID: https://orcid.org/0000-0001-9980-4225, Davis, S.J., Stockinger, H. ORCID: https://orcid.org/0000-0001-6404-4430, Schütz, G.J. and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Nature Immunology 19 (5): 487-496. May 2018

Extracellular purine metabolism is the switchboard of immunosuppressive macrophages and a novel target to treat diseases with macrophage imbalances.
Ohradanova-Repic, A., Machacek, C., Charvet, C., Lager, F., Le Roux, D., Platzer, R., Leksa, V., Mitulovic, G., Burkard, T.R., Zlabinger, G.J., Fischer, M.B., Feuillet, V., Renault, G., Blüml, S., Benko, M., Suchanek, M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Matsuyama, T., Cavaco-Paulo, A., Bismuth, G. and Stockinger, H.
Frontiers in Immunology 9 : 852. 27 April 2018

2017

A cellular platform for the evaluation of immune checkpoint molecules.
Jutz, S., Hennig, A., Paster, W., Asrak, Ö., Dijanovic, D., Kellner, F., Pickl, W.F., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Leitner, J. and Steinberger, P.
Oncotarget 8 (39): 64892-64906. 12 September 2017

Förster resonance energy transfer to study TCR-pMHC interactions in the immunological synapse.
Schütz, G.J. and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
In: The immune synapse. Methods in Molecular Biology, 1584 . Springer Nature, New York, NY, USA, 207-229. ISBN 978-1-4939-8327-8 3 March 2017

2016

RASGRP1 deficiency causes immunodeficiency with impaired cytoskeletal dynamics.
Salzer, E., Cagdas, D., Hons, M., Mace, E.M., Garncarz, W., Petronczki, Ö.Y., Platzer, R., Pfajfer, L., Bilic, I., Ban, S.A., Willmann, K.L., Mukherjee, M., Supper, V., Hsu, H.T., Banerjee, P.P., Sinha, P., McClanahan, F., Zlabinger, G.J., Pickl, W.F., Gribben, J.G., Stockinger, H., Bennett, K.L., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Dupré, L., Sanal, Ö., Jäger, U., Sixt, M., Tezcan, I., Orange, J.S. and Boztug, K.
Nature Immunology 17 (12): 1352-1360. December 2016

Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1.
Jutz, S., Leitner, J., Schmetterer, K., Doel-Perez, I., Majdic, O., Grabmeier-Pfistershammer, K., Paster, W., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Steinberger, P.
Journal of Immunological Methods 430 : 10-20. March 2016

Single molecule methods to measure receptor–ligand interaction in immunological synapses.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Schütz, G.J.
In: Encyclopedia of Cell Biology. Reference Module in Biomedical Sciences, 3 . Elsevier, 660-666. ISBN 978-0-12-394796-3 2016

2015

Single molecule fluorescence microscopy on planar supported bilayers.
Axmann, M., Schütz, G.J. and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Journal of Visualized Experiments (105): e53158. 31 October 2015

Measuring TCR-pMHC binding in situ using a FRET-based microscopy assay.
Axmann, M., Schütz, G.J. and Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198
Journal of Visualized Experiments (105): e53157. 30 October 2015

Rapid multiplex analysis of lipid raft components with single-cell resolution.
Schatzlmaier, P., Supper, V., Göschl, L., Zwirzitz, A., Eckerstorfer, P., Ellmeier, W., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Stockinger, H.
Science Signaling 8 (395): rs11. September 2015

2013

The interdisciplinary science of T-cell recognition.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Davis, M.M.
In: Advances in Immunology. Advances in Immunology, 119 . Elsevier, 1-50. ISBN 978-0-12-407707-2 22 July 2013

Distinct TCR signaling pathways drive proliferation and cytokine production in T cells.
Guy, C.S., Vignali, K.M., Temirov, J., Bettini, M.L., Overacre, A.E., Smeltzer, M., Zhang, H., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Tsai, Y.H., Lobry, C., Xie, J., Dempsey, P.J., Crawford, H.C., Aifantis, I., Davis, M.M. and Vignali, D.A.A.
Nature Immunology 14 (3): 262-70. March 2013

2012

Determination of interaction kinetics between the T cell receptor and peptide-loaded MHC class II via single-molecule diffusion measurements.
Axmann, M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Davis, M.M. and Schütz, G.J.
Biophysical Journal 103 (2): L17-9. 18 July 2012

Photocrosslinkable pMHC monomers stain T cells specifically and cause ligand-bound TCRs to be 'preferentially' transported to the cSMAC.
Xie, J., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Newell, E.W., Huang, J., Ebert, P.J.R., Li, Q.J. and Davis, M.M.
Nature Immunology 13 (7): 674-80. July 2012

2010

Evidence for a functional sidedness to the (α)(β)TCR.
Kuhns, M.S., Girvin, A.T., Klein, L.O., Chen, R., Jensen, K.D.C., Newell, E.W., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Lillemeier, B.F., Huse, M., Chien, Y.H., Garcia, K.C. and Davis, M.M.
Proceedings of the National Academy of Sciences of the United States of America 107 (11): 5094-9. 16 March 2010

TCR-peptide-MHC interactions in situ show accelerated kinetics and increased affinity.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Axmann, M., Mörtelmaier, M.A., Lillemeier, B.F., Newell, E.W., Brameshuber, M., Klein, L.O., Schütz, G.J. and Davis, M.M.
Nature 463 (7283): 963-7. 18 February 2010

TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation.
Lillemeier, B.F., Mörtelmaier, M.A., Forstner, M.B., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Groves, J.T. and Davis, M.M.
Nature Immunology 11 (1): 90-96. January 2010

Functional development of the T cell receptor for antigen.
Ebert, P.J.R., Li, Q.J., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Davis, M.M.
Progress in Molecular Biology and Translational Science 92 : 65-100. 2010

2007

T cells as a self-referential, sensory organ.
Davis, M.M., Krogsgaard, M., Huse, M., Huppa, J. ORCID: https://orcid.org/0000-0003-2634-8198, Lillemeier, B.F. and Li, Q.J.
Annual Review of Immunology 25 : 681-95. 2 January 2007

2005

Agonist/endogenous peptide-MHC heterodimers drive T cell activation and sensitivity.
Krogsgaard, M., Li, Q.J., Sumen, C., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Huse, M. and Davis, M.M.
Nature 434 (7030): 238-43. 10 March 2005

Quantitative imaging of lymphocyte membrane protein reorganization and signaling.
Kasson, P.M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Krogsgaard, M., Davis, M.M. and Brunger, A.T.
Biophysical Journal 88 (1): 579-89. 1 January 2005

A hybrid machine-learning approach for segmentation of protein localization data.
Kasson, P.M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Davis, M.M. and Brunger, A.T.
Bioinformatics 21 (19): 3778-86. January 2005

2004

CD4 enhances T cell sensitivity to antigen by coordinating Lck accumulation at the immunological synapse.
Li, Q.J., Dinner, A.R., Qi, S., Irvine, D.J., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Davis, M.M. and Chakraborty, A.K.
Nature Immunology 5 (8): 791-9. 1 August 2004

T cell killing does not require the formation of a stable mature immunological synapse.
Purbhoo, M.A., Irvine, D.J., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Davis, M.M.
Nature Immunology 5 (5): 524-530. 1 May 2004

Quantitative analysis of lymphocyte membrane protein redistribution from fluorescence microscopy.
Kasson, P.M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Davis, M.M. and Brunger, A.T.
In: International Conference on Image Processing, ICIP 2004, 18-21 Oct 2004. 2004

Quantitative analysis of membrane protein localization and signaling.
Kasson, P.M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Davis, M.M. and Brunger, A.T.
In: IEEE Computational Systems Bioinformatics Conference, CSB 20041, 16-19 Aug 2004. 2004

2003

T-cell-antigen recognition and the immunological synapse.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Davis, M.M.
Nature Reviews Immunology 3 (12): 973-83. 1 December 2003

Linking molecular and cellular events in T-cell activation and synapse formation.
Krogsgaard, M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Purbhoo, M.A. and Davis, M.M.
Seminars in Immunology 15 (6): 307-15. December 2003

Continuous T cell receptor signaling required for synapse maintenance and full effector potential.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Gleimer, M., Sumen, C. and Davis, M.M.
Nature Immunology 4 (8): 749-55. 1 August 2003

Linker for activation of T cells, zeta-associated protein-70, and Src homology 2 domain-containing leukocyte protein-76 are required for TCR-induced microtubule-organizing center polarization.
Kuhné, M.R., Lin, J., Yablonski, D., Mollenauer, M.N., Ehrlich, L.I.R., Huppa, J. ORCID: https://orcid.org/0000-0003-2634-8198, Davis, M.M. and Weiss, A.
Journal of Immunology 171 (2): 860-6. 15 July 2003

Dynamics of cell surface molecules during T cell recognition.
Davis, M.M., Krogsgaard, M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Sumen, C., Purbhoo, M.A., Irvine, D.J., Wu, L.C. and Ehrlich, L.
Annual review of biochemistry 72 : 717-42. July 2003

1998

Dislocation of type I membrane proteins from the ER to the cytosol is sensitive to changes in redox potential.
Tortorella, D., Story, C.M., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Wiertz, E.J., Jones, T.R. and Ploegh, H.L.
Journal of Cell Biology 142 (2): 365-76. 27 July 1998

The eS-Sence of -SH in the ER.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Ploegh, H.L.
Cell 92 (2): 145-8. 23 January 1998

1997

In vitro translation and assembly of a complete T cell receptor-CD3 complex.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Ploegh, H.L.
Journal of Experimental Medicine 186 (3): 393-403. 4 August 1997

The alpha chain of the T cell antigen receptor is degraded in the cytosol.
Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198 and Ploegh, H.L.
Immunity 7 (1): 113-22. 1 July 1997

A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface.
Kleijnen, M.F., Huppa, J.B. ORCID: https://orcid.org/0000-0003-2634-8198, Lucin, P., Mukherjee, S., Farrell, H., Campbell, A.E., Koszinowski, U.H., Hill, A.B. and Ploegh, H.L.
EMBO Journal 16 (4): 685-94. 15 February 1997