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| Item Type: | Article |
|---|---|
| Title: | Spatial-temporal diversity of extrachromosomal DNA shapes urothelial carcinoma evolution and tumor-immune microenvironment |
| Creators Name: | Lv, W., Zeng, Y., Li, C., Liang, Y., Tao, H., Zhu, Y., Sui, X., Li, Y., Jiang, S., Gao, Q., Rodriguez-Fos, E., Prasad, G., Wang, Y., Zhou, R., Xu, Z., Pan, X., Chen, L., Xiang, X., Teng, H., Sun, C., Qin, T., Dong, W., Li, Y., Lan, X., Li, X., Lin, L., Bolund, L., Yang, H., Verhaak, R.G.W., Faltas, B.M., Hansen, J.B., Wu, S., Mischel, P.S., Henssen, A.G., Bafna, V., Luebeck, J., Regenberg, B., Luo, Y., Lin, C. and Han, P. |
| Abstract: | Extrachromosomal DNA (ecDNA) presents a promising target for cancer therapy; however, its spatial–temporal diversity and influence on tumor evolution and the immune microenvironment remain largely unclear. We apply computational methods to analyze ecDNA from whole-genome sequencing data of 595 patients with urothelial carcinoma. We demonstrate that ecDNA drives clonal evolution through structural rearrangements during malignant transformation and recurrence of urothelial carcinoma. This supports a model wherein tumors evolve via the selective expansion of ecDNA-bearing cells. Through multiregional sampling of tumors, we demonstrate that ecDNA contributes to the evolution of multifocality and increased intratumoral heterogeneity. ecDNA is present in 36% of urothelial carcinoma tumors and correlates with an immunosuppressive phenotype and poor prognosis. Single-cell RNA sequencing analyses reveal that ecDNA(+) malignant cells exhibit diminished expression of MHC class I molecules, enabling them to evade T-cell immunity. Finally, we show that sequencing of urinary sediment–derived DNA has excellent specificity in detecting ecDNA. SIGNIFICANCE: Our comprehensive analysis of ecDNA in urothelial carcinoma reveals its crucial role in driving the evolution and heterogeneity of multifocal cancer, as well as its early involvement in tumorigenesis. Moreover, this study sheds light on immune evasion mechanisms associated with ecDNA and offers valuable insights for developing targeted therapeutic strategies. |
| Keywords: | Urothelial Carcinoma, Extrachromosomal DNA, Cancer Genetics, Tumor Heterogeneity, Tumor Evolution, Tumor Microenvironment, Liquid Biopsy |
| Source: | Cancer Discovery |
| ISSN: | 2159-8274 |
| Publisher: | American Association for Cancer Research |
| Volume: | 15 |
| Number: | 6 |
| Page Range: | 1225-1246 |
| Date: | 3 June 2025 |
| Official Publication: | https://doi.org/10.1158/2159-8290.cd-24-1532 |
| PubMed: | View item in PubMed |
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