Article

Protocol for high-resolution 3D spatial transcriptomics using Open-ST.
Schott, M. ORCID: https://orcid.org/0009-0000-7393-2421, León-Periñán, D. ORCID: https://orcid.org/0000-0002-7970-0362, Splendiani, E., Ferretti, E., Macino, G., Karaiskos, N. ORCID: https://orcid.org/0000-0001-7771-3947 and Rajewsky, N. ORCID: https://orcid.org/0000-0002-4785-4332
STAR Protocols 6 (1): 103521. 21 March 2025

Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry.
Gallaccio, G. ORCID: https://orcid.org/0000-0002-1060-5244, Wang, M., Schlickeiser, S., Kunkel, D., Böttcher, C. ORCID: https://orcid.org/0000-0002-6226-586X and Fernández-Zapata, C. ORCID: https://orcid.org/0000-0003-4795-6053
STAR Protocols 5 (2): 103038. 27 April 2024

Protocol for assessing myogenic tone and perfusion pressure in isolated mouse kidneys.
Chu, Z. ORCID: https://orcid.org/0009-0000-9084-8942, Kassmann, M. ORCID: https://orcid.org/0000-0002-4785-6530, Anistan, Y.M., Luft, F.C. ORCID: https://orcid.org/0000-0002-8635-1199, Gollasch, M. ORCID: https://orcid.org/0000-0003-2797-1934 and Tsvetkov, D. ORCID: https://orcid.org/0000-0002-6472-971X
STAR Protocols 5 (1): 102845. 15 March 2024

Using PrISMa to reveal the interactome of the human claudins family.
Perez-Hernandez, D. ORCID: https://orcid.org/0000-0003-0266-9050, Suarez-Artiles, L. ORCID: https://orcid.org/0000-0001-7991-5446, Jones, M.S.O. and Dittmar, G. ORCID: https://orcid.org/0000-0003-3647-8623
STAR Protocols 4 (4): 102549. 15 December 2023

ASSURED-optimized CRISPR protocol for knockout/SNP knockin in hiPSCs.
Ludwik, K.A. ORCID: https://orcid.org/0000-0002-9436-7270, Telugu, N. ORCID: https://orcid.org/0000-0003-4509-7056, Schommer, S., Stachelscheid, H. and Diecke, S. ORCID: https://orcid.org/0000-0002-5219-5992
STAR Protocols 4 (3): 102406. 15 September 2023

Protocol to dissociate healthy and infected murine- and hamster-derived lung tissue for single-cell transcriptome analysis.
Pennitz, P., Goekeri, C., Trimpert, J., Wyler, E. ORCID: https://orcid.org/0000-0002-9884-1806, Ebenig, A., Weissfuss, C., Mühlebach, M.D., Witzenrath, M. and Nouailles, G. ORCID: https://orcid.org/0000-0003-1973-3119
STAR Protocols 4 (1): 101957. 17 March 2023

Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae.
Corradi, L. ORCID: https://orcid.org/0000-0001-8837-6544, Zaupa, M. ORCID: https://orcid.org/0000-0003-3562-9020, Sawamiphak, S. ORCID: https://orcid.org/0000-0003-2298-3565 and Filosa, A. ORCID: https://orcid.org/0000-0003-2599-6951
STAR Protocols 3 (4): 101731. 16 December 2022

Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions.
Giacomello, G., Böttcher, C. ORCID: https://orcid.org/0000-0002-6226-586X and Parr, M.K.
STAR Protocols 3 (4): 101715. 16 December 2022

Protocol for fast scRNA-seq raw data processing using scKB and non-arbitrary quality control with COPILOT.
Hsu, C.W. ORCID: https://orcid.org/0000-0003-3709-9748, Shahan, R., Nolan, T.M., Benfey, P.N. and Ohler, U. ORCID: https://orcid.org/0000-0002-0881-3116
STAR Protocols 3 (4): 101729. 16 December 2022

High-content analysis of neuronal morphology in human iPSC-derived neurons.
Lickfett, S., Menacho, C., Zink, A. ORCID: https://orcid.org/0000-0002-8489-6339, Telugu, N.S. ORCID: https://orcid.org/0000-0003-4509-7056, Beller, M., Diecke, S. ORCID: https://orcid.org/0000-0002-5219-5992, Cambridge, S. and Prigione, A. ORCID: https://orcid.org/0000-0001-9457-1952
STAR Protocols 3 (3): 101567. 16 September 2022

Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry.
Menzel, L. ORCID: https://orcid.org/0000-0002-3816-6255, Zschummel, M. ORCID: https://orcid.org/0000-0002-4778-8742 and Rehm, A. ORCID: https://orcid.org/0000-0001-7490-1950
STAR Protocols 3 (2): 101267. 17 June 2022

Protocol for efficient CRISPR/Cas9/AAV-mediated homologous recombination in mouse hematopoietic stem and progenitor cells.
Tran, N.T. ORCID: https://orcid.org/0000-0003-0896-8641, Trombke, J., Rajewsky, K. ORCID: https://orcid.org/0000-0002-6633-6370 and Chu, V.T. ORCID: https://orcid.org/0000-0003-1327-8696
STAR Protocols 1 (1): 100028. 19 June 2020

Review

A concise guide to choosing suitable gene expression systems for recombinant protein production.
Schütz, A. ORCID: https://orcid.org/0000-0002-0606-2574, Bernhard, F., Berrow, N., Buyel, J.F., Ferreira-da-Silva, F., Haustraete, J., van den Heuvel, J., Hoffmann, J.E., de Marco, A., Peleg, Y., Suppmann, S., Unger, T., Vanhoucke, M., Witt, S. and Remans, K.
STAR Protocols 4 (4): 102572. 15 December 2023