Browse by Research Team

2008

The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.
Krauss, N., Wessner, H., Welfle, K., Welfle, H., Scholz, C., Seifert, M., Zubow, K., Ay, J., Hahn, M., Scheerer, P., Skerra, A. and Hoehne, W.
Proteins: Structure, Function, and Bioinformatics 73 (3): 552-565. 15 November 2008

2007

Helicobacter exploits integrin for type IV secretion and kinase activation.
Kwok, T., Zabler, D., Urman, S., Rohde, M., Hartig, R., Wessler, S., Misselwitz, R., Berger, J.M., Sewald, N., Koenig, W. and Backert, S.
Nature 449 (7164): 862-866. 18 October 2007

Binding of regulatory protein omega from Streptococcus pyogenes plasmid pSM19035 to direct and inverted 7-base pair repeats of operator DNA.
Dostal, L., Pratto, F., Alonso, J.C. and Welfle, H.
Journal of Raman Spectroscopy 38 (2): 166-175. February 2007

2006

Mn2+-DNA interactions in aqueous systems: A Raman spectroscopic study.
Muntean, C.M., Misselwitz, R., Dostal, L. and Welfle, H.
Spectroscopy 20 (1): 29-35. January 2006

The influence of Mn2+ on DNA structure in the presence of Na+ ions: a Raman spectroscopic study.
Muntean, C.M., Misselwitz, R. and Welfle, H.
Spectroscopy 20 (5-6): 261-268. 2006

2005

DNA structure at low pH values, in the presence of Mn2+ ions: a Raman study.
Muntean, C.M., Dostal, L., Misselwitz, R. and Welfle, H.
Journal of Raman Spectroscopy 36 (11): 1047-1051. November 2005

Role of the N-terminal region and of beta-sheet residue Thr29 on the activity of the omega(2) global regulator from the broad-host range Streptococcus pyogenes plasmid pSM19035.
Welfle, K., Pratto, F., Misselwitz, R., Behlke, J., Alonso, J.C. and Welfle, H.
Biological Chemistry 386 (9): 881-894. 1 September 2005

Arc repressor-operator DNA interactions and contribution of Phe10 to binding specificity.
Dostal, L., Misselwitz, R. and Welfle, H.
Biochemistry 44 (23): 8387-8396. 1 January 2005

Thermodynamic and structural equivalence of two HLA-B27 subtypes complexed with a self-peptide.
Huelsmeyer, M., Welfle, K., Poehlmann, T., Misselwitz, R., Alexiev, U., Welfle, H., Saenger, W., Uchanska-Ziegler, B. and Ziegler, A.
Journal of Molecular Biology 346 (5): 1367-1379. 1 January 2005

2004

Partial B-to-A DNA transition upon minor groove binding of protein Sac7d monitored by Raman spectroscopy.
Dostal, L., Chen, C.Y., Wang, A.H. and Welfle, H.
Biochemistry 43 (30): 9600-9609. 3 August 2004

Recognition of DNA by omega protein from the broad-host range Streptococcus pyogenes plasmid pSM19035: analysis of binding to operator DNA with one to four heptad repeats.
de la Hoz, A.B., Pratto, F., Misselwitz, R., Speck, C., Weihofen, W., Welfle, K., Saenger, W., Welfle, H. and Alonso, J.C.
Nucleic Acids Research 32 (10): 3136-3147. 9 June 2004

Thermodynamic and structural analysis of peptide- and allele-dependent properties of two HLA-B27 subtypes exhibiting differential disease association.
Hillig, R.C., Huelsmeyer, M., Saenger, W., Welfle, K., Misselwitz, R., Welfle, H., Kozerski, C., Volz, A., Uchanska-Ziegler, B. and Ziegler, A.
Journal of Biological Chemistry 279 (1): 652-663. 1 January 2004

2003

Design and characterization of a hybrid miniprotein that specifically inhibits porcine pancreatic elastase.
Hilpert, K., Wessner, H., Schneider-Mergener, J., Welfle, K., Misselwitz, R., Welfle, H., Hocke, A.C., Hippenstiel, S. and Hoehne, W.
Journal of Biological Chemistry 278 (27): 24986-24993. 4 July 2003

X-ray structure of the cyclomaltohexaicosaose triiodide inclusion complex provides a model for amylose-iodine at atomic resolution.
Nimz, O., Gessler, K., Uson, I., Laettig, S., Welfle, H., Sheldrick, G.M. and Saenger, W.
Carbohydrate Research 338 (9): 977-986. 22 April 2003

Interaction of epitope-related and -unrelated peptides with anti-p24 (HIV-1) monoclonal antibody CB4-1 and its Fab fragment.
Welfle, K., Misselwitz, R., Hoehne, W. and Welfle, H.
Journal of Molecular Recognition 16 (1): 54-62. 1 January 2003

RP4 repressor protein KorB binds to the major groove of the operator DNA: A Raman study.
Dostal, L., Khare, D., Bok, J., Heinemann, U., Lanka, E. and Welfle, H.
Biochemistry 42 (49): 14476-14482. 1 January 2003

Raman spectroscopy of regulatory protein Omega from Streptococcus pyogenes plasmid pSM19035 and complexes with operator DNA.
Dostal, L., Misselwitz, R., Laettig, S., Alonso, J.C. and Welfle, H.
Spectroscopy 17 (2-3): 435-445. 2003

2002

The cystine knot promotes folding and not thermodynamic stability in vascular endothelial growth factor.
Muller, Y.A., Heiring, C., Misselwitz, R., Welfle, K. and Welfle, H.
Journal of Biological Chemistry 277 (45): 43410-43416. 8 November 2002

In vitro and in vivo stability of the epsilon2zeta2 protein complex of the broad host-range Streptococcus pyogenes pSM19035 addiction system.
Camacho, A.G., Misselwitz, R., Behlke, J., Ayora, S., Welfle, K., Meinhart, A., Lara, B., Saenger, W., Welfle, H. and Alonso, J.C.
Biological Chemistry 383 (11): 1701-1713. November 2002

NblA from Anabaena sp PCC 7120 is a mostly alpha-helical protein undergoing reversible trimerization in solution.
Strauss, H., Misselwitz, R., Labudde, D., Nicklisch, S. and Baier, K.
European Journal of Biochemistry 269 (18): 4617-4624. 1 January 2002

2001

Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
Hilpert, K., Hansen, G., Wessner, H., Kuettner, G., Welfle, K., Seifert, M. and Hoehne, W.
Protein Engineering 14 (10): 803-806. 1 January 2001

Cross-reactive binding of cyclic peptides to an anti-TGFalpha antibody fab fragment: an X-ray structural and thermodynamic analysis.
Hahn, M., Winkler, D., Welfle, K., Misselwitz, R., Welfle, H., Wessner, H., Zahn, G., Scholz, C., Seifert, M., Harkins, R., Schneider-Mergener, J. and Hoehne, W.G.
Journal of Molecular Biology 314 (2): 293-309. 1 January 2001

Efficient eukaryotic expression system for authentic human sex hormone-binding globulin.
Hilpert, J., Vorum, H., Burmeister, R., Spoelgen, R., Grishkovskaya, I., Misselwitz, R., Nykjaer, A. and Willnow, T.E.
Biochemical Journal 360 (3): 609-615. 1 January 2001

Interaction of a designed interleukin-10 epitope mimic with an antibody studied by isothermal titration microcalorimetry.
Welfle, K., Misselwitz, R., Sabat, R., Volk, H.D., Schneider-Mergener, J., Reineke, U. and Welfle, H.
Journal of Molecular Recognition 14 (2): 89-98. 1 January 2001

Stability and DNA-binding properties of the omega regulator protein from the broad-host range Streptococcus pyogenes plasmid pSM19035.
Misselwitz, R., de la Hoz, A.B., Ayora, S., Welfle, K., Behlke, J., Murayama, K., Saenger, W., Alonso, J.C. and Welfle, H.
FEBS Letters 505 (3): 436-440. 1 January 2001

2000

Interaction of fMet-tRNAfMet with the C-terminal domain of translational initiation factor IF2 from Bacillus stearothermophilus.
Krafft, C., Diehl, A., Laettig, S., Behlke, J., Heinemann, U., Pon, C.L., Gualerzi, C.O. and Welfle, H.
FEBS Letters 471 (2-3): 128-132. 14 April 2000

Structural heterogeneity in intramolecular DNA triple helices.
Weisz, K., Leitner, D., Krafft, C. and Welfle, H.
Biological Chemistry 381 (4): 275-283. 1 April 2000

The C-terminal subdomain (IF2 C-2) contains the entire fMet-tRNA binding site of initiation factor IF2.
Spurio, R., Brandi, L., Caserta, E., Pon, C.L., Gualerzi, C.O., Misselwitz, R., Krafft, C., Welfle, K. and Welfle, H.
Journal of Biological Chemistry 275 (4): 2447-2454. 28 January 2000

Expression and purification of dynamin II domains and initial studies on structure and function.
Dong, J., Misselwitz, R., Welfle, H. and Westermann, P.
Protein Expression and Purification 20 (2): 314-323. 1 January 2000

1999

The fMet-tRNA binding domain of translational initiation factor IF2: role and environment of its two Cys residues.
Misselwitz, R., Welfle, K., Krafft, C., Welfle, H., Brandi, L., Caserta, E. and Gualerzi, C.O.
FEBS Letters 459 (3): 332-336. 15 October 1999

Conformation, pH-induced conformational changes, and thermal unfolding of anti-p24 (HIV-1) monoclonal antibody CB4-1 and its Fab and Fc fragments.
Welfle, K., Misselwitz, R., Hausdorf, G., Hoehne, W. and Welfle, H.
Biochimica et Biophysica Acta 1431 : 120-131. 12 April 1999

A synthetic mimic of a discontinuous binding site on interleukin-10.
Reineke, U., Sabat, R., Misselwitz, R., Welfle, H., Volk, H.D. and Schneider-Mergener, J.
Nature Biotechnology 17 : 271-275. 1 March 1999

Preliminary characterization by X-ray diffraction and Raman spectroscopy of a crystalline complex of Bacillus stearothermophilus initiation factor 2 C-domain and fMet-tRNA(fMet).
Foerster, C., Krafft, C., Welfle, H., Gualerzi, C.O. and Heinemann, U.
Acta Crystallographica Section D 55 Pt. 3 : 712-716. 1 March 1999

Interaction of the capsid protein p24 (HIV-1) with sequence-derived peptides: influence on p24 dimerization.
Hilpert, K., Behlke, J., Scholz, C., Misselwitz, R., Schneider-Mergener, J. and Hoehne, W.
Virology 254 : 6-10. 1 January 1999

1998

The laminarinase from thermophilic eubacterium Rhodothermus marinus - Conformation, stability, and identification of active site carboxylic residues by site-directed mutagenesis.
Krah, M., Misselwitz, R., Politz, O., Thomsen, K.K., Welfle, H. and Borriss, R.
European Journal of Biochemistry 257 : 101-111. 1 October 1998

Melting points of lysozyme and ribonuclease A crystals correlated with protein unfolding: a Raman spectroscopic study.
Jacob, J., Krafft, C., Welfle, K., Welfle, H. and Saenger, W.
Acta Crystallographica Section D 54 : 74-80. 1 January 1998

Raman spectroscopic analysis of Tet repressor - operator DNA interaction in deuterium oxide.
Krafft, C., Hinrichs, W., Orth, P., Saenger, W. and Welfle, H.
Cellular and Molecular Biology 44 : 239-250. 1 January 1998

Interaction of Tet repressor with operator DNA and with tetracycline studied by infrared and Raman spectroscopy.
Krafft, C., Hinrichs, W., Orth, P., Saenger, W. and Welfle, H.
Biophysical Journal 74 (1): 63-71. January 1998

1997

C repeats of the streptococcal M1 protein achieve the human serum albumin binding ability by flanking regions which stabilize the coiled-coil conformation.
Gubbe, K., Misselwitz, R., Welfle, K., Reichardt, W., Schmidt, K.H. and Welfle, H.
Biochemistry 36 : 8107-8113. 1 January 1997

Conformation and stability of streptokinases from nephritogenic and nonnephritogenic strains of Streptococci.
Welfle, K., Misselwitz, R., Schaup, A., Gerlach, D. and Welfle, H.
Proteins: Structure, Function, and Bioinformatics 27 : 26-35. 1 January 1997

Translational initiation factor IF2 from Bacillus stearothermophilus: a spectroscopic and microcalorimetric study of the C-domain.
Misselwitz, R., Welfle, K., Krafft, C., Gualerzi, C.O. and Welfle, H.
Biochemistry 36 : 3170-3178. 1 January 1997

1996

Chemical modification of recombinant HIV-1 capsid protein p24 leads to the release of a hidden epitope prior to changes of the overall folding of the protein.
Ehrhard, B., Misselwitz, R., Welfle, K., Hausdorf, G., Glaser, R.W., Schneider-Mergener, J. and Welfle, H.
Biochemistry 35 : 9097-9105. 1 January 1996

Individual amino acids in the N-terminal loop region determine the thermostability and unfolding characteristics of bacterial glucanases.
Welfle, K., Misselwitz, R., Politz, O., Borriss, R. and Welfle, H.
Protein Science 5 : 2255-2265. 1 January 1996

1995

Conformation and stability of recombinant HIV-1 capsid protein p24 (rp24).
Misselwitz, R., Hausdorf, G., Welfle, K., Hoehne, W.E. and Welfle, H.
Biochimica et Biophysica Acta 1250 (1): 9-18. 1 January 1995

Influence of Ca2+ on conformation and stability of three bacterial hybrid glucanases.
Welfle, K., Misselwitz, R., Welfle, H., Politz, O. and Borriss, R.
European Journal of Biochemistry 229 (3): 726-735. 1 January 1995

Stability of Escherichia coli single-stranded DNA binding protein (EcoSSB).
Misselwitz, R., Welfle, K., Curth, U., Urbanke, C. and Welfle, H.
Journal of Biomolecular Structure & Dynamics 12 (5): 1041-1054. 1 January 1995

1994

Conformations and stabilities of human Glu1 and Lys78- plasminogen and of the fragments mini- and microplasminogen, analysed by circular dichroism and differential scanning calorimetry.
Misselwitz, R., Welfle, K. and Welfle, H.
International Journal of Biological Macromolecules 16 : 187-194. 1 January 1994

Microcalorimetric deterimination of the thermostability of three hybrid (1-3,1-4)-beta-glucanases.
Welfle, K., Misselwitz, R., Welfle, H., Simon, O., Politz, O. and Borriss, R.
Journal of Biomolecular Structure & Dynamics 11 : 1417-1424. 1 January 1994

The thermostability of natural variants of bacterial plasminogen- activator staphylokinase.
Gase, A., Birch-Hirschfeld, E., Guehrs, K.H., Hartmann, M., Vetterman, S., Damaschun, G., Damaschun, H., Gast, K., Misselwitz, R., Zirwer, D., Collen, D. and Schlott, B.
European Journal of Biochemistry 223 : 303-308. 1 January 1994

1993

Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast.
Damaschun, G., Damaschun, H., Gast, K., Misselwitz, R., Mueller, J.J., Pfeil, W. and Zirwer, D.
Biochemistry 32 (30): 7739-7746. 3 August 1993

Conformation of d (GGGATCCC) 2 in crystals and in solution studied by X-ray diffraction, Raman spectroscopy and molecular modelling.
Fabian, H., Hoelzer, W., Heinemann, U., Sklenar, H. and Welfle, H.
Nucleic Acids Research 21 (3): 569-576. 11 February 1993

Cold denaturation of yeast phosphoglycerate kinase: kinetics of changes in secondary structure and compactness on unfolding and refolding.
Gast, K., Damaschun, G., Damaschun, H., Misselwitz, R. and Zirwer, D.
Biochemistry 32 : 7747-7752. 1 January 1993

Conformational stability of the DNA-binding histone-like protein, HBsu, from Bacillus subtilis, and of the four HBsu variants [F29W],[F47W],[F50W] and [F79W].
Welfle, H., Welfle, K., Misselwitz, R., Groch, N. and Heinemann, U.
Journal of Biomolecular Structure & Dynamics 11 : 381-394. 1 January 1993

Conformations and conformational changes of four Phe->Trp variants of the DNA-binding histone-like protein, HBsu, from Bacillus subtilis studied by circular dichroism and fluorescence spectroscopy.
Welfle, H., Misselwitz, R., Welfle, K., Schindelin, H., Scholtz, A.S. and Heinemann, U.
European Journal of Biochemistry 217 : 849-856. 1 January 1993

Physical and conformational properties of staphylokinase in solution.
Damaschun, G., Damaschun, H., Gast, K., Misselwitz, R., Zirwer, D., Guehrs, K.H., Hartmann, M., Schlott, B., Triebel, H. and Behnke, H.
Biochimica et Biophysica Acta 1161 : 244-248. 1 January 1993

1992

Secondary structure of streptokinase in aqueous solution - a fourier transform infrared spectroscopic study.
Fabian, H., Naumann, D., Misselwitz, R., Ristau, O., Gerlach, D. and Welfle, H.
Biochemistry 31 (28): 6532-6538. 21 July 1992

Conformational properties of streptokinase - differential scanning calorimetric investigations.
Welfle, K., Pfeil, W., Misselwitz, R., Welfle, H. and Gerlach, D.
International Journal of Biological Macromolecules 14 : 19-22. 1 January 1992

Conformational properties of streptokinase - secondary structure and localization of aromatic amino acids.
Welfle, H., Misselwitz, R., Fabian, H., Damerau, W., Hoelzer, W., Gerlach, D., Kalnin, N.N. and Venyaminov, S.Y.
International Journal of Biological Macromolecules 14 : 9-18. 1 January 1992

Limited proteolysis of streptokinase and properties of some fragments.
Misselwitz, R., Kraft, R., Kostka, S., Fabian, H., Welfle, K., Pfeil, W., Welfle, H. and Gerlach, D.
International Journal of Biological Macromolecules 14 : 107-116. 1 January 1992

Salt-dependent and protein-concentration-dependent changes in the solution structure of DNA-binding histone-like protein, HBsn, from Bacillus subtilis.
Welfle, H., Misselwitz, R., Welfle, K., Groch, N. and Heinemann, U.
European Journal of Biochemistry 204 : 1049-1055. 1 January 1992

Streptokinase is a flexible multi-domain protein.
Damaschun, G., Damaschun, H., Gast, K., Gerlach, D., Misselwitz, R., Welfle, H. and Zirwer, D.
European Biophysics Journal with Biophysics Letters 20 : 355-361. 1 January 1992