Browse by Research Team

Article

AI-guided pipeline for protein-protein interaction drug discovery identifies an SARS-CoV-2 inhibitor.
Trepte, P., Secker, C., Olivet, J., Blavier, J., Kostova, S., Maseko, S.B., Minia, I., Silva Ramos, E., Cassonnet, P., Golusik, S., Zenkner, M., Beetz, S., Liebich, M.J., Scharek, N., Schutz, A., Sperling, M., Lisurek, M., Wang, Y., Spirohn, K., Hao, T., Calderwood, M.A., Hill, D.E., Landthaler, M., Choi, S.G., Twizere, J.C., Vidal, M. and Wanker, E.E.
Molecular Systems Biology 20 (4): 428-457. 2 April 2024

Structure-based design of xanthine-imidazopyridines and -imidazothiazoles as highly potent and in vivo efficacious tryptophan hydroxylase inhibitors.
Specker, E., Wesolowski, R., Schütz, A., Matthes, S., Mallow, K., Wasinska-Kalwa, M., Winkler, L., Oder, A., Alenina, N., Pleimes, D., von Kries, J.P., Heinemann, U., Bader, M. and Nazaré, M.
Journal of Medicinal Chemistry 66 (21): 14866-14896. 9 November 2023

An unconventional gatekeeper mutation sensitizes inositol hexakisphosphate kinases to an allosteric inhibitor.
Aguirre, T., Hostachy, S., Dornan, G.L., Neuenschwander, M., Seyffarth, C., Haucke, V., Schütz, A., von Kries, J.P. and Fiedler, D.
eLife 21 : RP88982. 16 October 2023

IFNγ binding to extracellular matrix prevents fatal systemic toxicity.
Kemna, J., Gout, E., Daniau, L., Lao, J., Weißert, K., Ammann, S., Kühn, R., Richter, M., Molenda, C., Sporbert, A., Zocholl, D., Klopfleisch, R., Schütz, A., Lortat-Jacob, H., Aichele, P., Kammertoens, T. and Blankenstein, T.
Nature Immunology 24 (3): 414-422. March 2023

Structure-based design of xanthine-benzimidazole derivatives as novel and potent tryptophan hydroxylase inhibitors.
Specker, E., Matthes, S., Wesolowski, R., Schütz, A., Grohmann, M., Alenina, N., Pleimes, D., Mallow, K., Neuenschwander, M., Gogolin, A., Weise, M., Pfeifer, J., Ziebart, N., Heinemann, U., von Kries, J.P., Nazaré, M. and Bader, M.
Journal of Medicinal Chemistry 65 (16): 11126-11149. 25 August 2022

Community-wide experimental evaluation of the PROSS stability-design method.
Peleg, Y., Vincentelli, R., Collins, B.M., Chen, K.E., Livingstone, E.K., Weeratunga, S., Leneva, N., Guo, Q., Remans, K., Perez, K., Bjerga, G.E.K., Larsen, Ø., Vaněk, O., Skořepa, O., Jacquemin, S., Poterszman, A., Kjaer, S., Christodoulou, E., Albeck, S., Dym, O., Ainbinder, E., Unger, T., Schuetz, A., Matthes, S., Bader, M., de Marco, A., Storici, P., Semrau, M.S., Stolt-Bergner, P., Aigner, C., Suppmann, S., Goldenzweig, A. and Fleishman, S.J.
Journal of Molecular Biology 433 (13): 166964. 25 June 2021

Phenylalanine-hydroxylase contributes to serotonin synthesis in mice.
Mordhorst, A., Dhandapani, P., Matthes, S., Mosienko, V., Rothe, M., Todiras, M., Self, J., Schunck, W.H., Schütz, A., Bader, M. and Alenina, N.
FASEB Journal 35 (6): e21648. June 2021

Structural basis of gene regulation by the Grainyhead/CP2 transcription factor family.
Ming, Q., Roske, Y., Schuetz, A., Walentin, K., Ibraimi, I., Schmidt-Ott, K.M. and Heinemann, U.
Nucleic Acids Research 46 (4): 2082-2095. 28 February 2018

Different inhibitory potencies of oseltamivir carboxylate, zanamivir, and several tannins on bacterial and viral neuraminidases as assessed in a cell-free fluorescence-based enzyme inhibition assay.
Quosdorf, S., Schuetz, A. and Kolodziej, H.
Molecules 22 (11): 1989. 17 November 2017

Crystal structure of the tricellulin C-terminal coiled-coil domain reveals a unique mode of dimerization.
Schuetz, A., Radusheva, V., Krug, S.M. and Heinemann, U.
Annals of the New York Academy of Sciences 1405 (1): 147-159. October 2017

Tricellulin is a target of the ubiquitin ligase Itch.
Jennek, S., Mittag, S., Reiche, J., Westphal, J.K., Seelk, S., Dörfel, M.J., Pfirrmann, T., Friedrich, K., Schütz, A., Heinemann, U. and Huber, O.
Annals of the New York Academy of Sciences 1397 (1): 157-168. June 2017

Functional interaction of CCAAT/enhancer-binding-protein-α basic region mutants with E2F transcription factors and DNA.
Kowenz-Leutz, E., Schuetz, A., Liu, Q., Knoblich, M., Heinemann, U. and Leutz, A.
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms 1859 (7): 841-847. July 2016

RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-κB pathway.
Murakawa, Y., Hinz, M., Mothes, J., Schuetz, A., Uhl, M., Wyler, E., Yasuda, T., Mastrobuoni, G., Friedel, C.C., Dölken, L., Kempa, S., Schmidt-Supprian, M., Blüthgen, N., Backofen, R., Heinemann, U., Wolf, J., Scheidereit, C. and Landthaler, M.
Nature Communications 6 : 7367. 14 July 2015

Light-switched inhibitors of protein tyrosine phosphatase PTP1B based on phosphonocarbonyl phenylalanine as photoactive phosphotyrosine mimetic.
Wagner, S., Schütz, A. and Rademann, J.
Bioorganic and Medicinal Chemistry 23 (12): 2839-2847. 15 June 2015

Roquin binding to target mRNAs involves a winged helix-turn-helix motif.
Schuetz, A., Murakawa, Y., Rosenbaum, E., Landthaler, M. and Heinemann, U.
Nature Communications 5 : 5701. 11 December 2014

Benzoylphosphonate-based photoactive phosphopeptide mimetics for modulation of protein tyrosine phosphatases and highly specific labeling of SH2 domains.
Horatscheck, A., Wagner, S., Ortwein, J., Kim, B.G., Lisurek, M., Beligny, S., Schuetz, A. and Rademann, J.
Angewandte Chemie International Edition 51 (37): 9441-9447. 10 September 2012

Molecular insights into arrhythmogenic right ventricular cardiomyopathy caused by plakophilin-2 missense mutations.
Kirchner, F., Schuetz, A., Boldt, L.H., Martens, K., Dittmar, G., Haverkamp, W., Thierfelder, L., Heinemann, U. and Gerull, B.
Circulation Cardiovascular Genetics 5 (4): 400-411. 1 August 2012

The Lin28 cold-shock domain remodels pre-let-7 microRNA.
Mayr, F., Schütz, A., Dge, N. and Heinemann, U.
Nucleic Acids Research 40 (15): 7492-7506. 1 August 2012

Structural and biochemical basis of Yos9 protein dimerization and possible contribution to self-association of 3-hydroxy-3-methylglutaryl-coenzyme A reductase degradation ubiquitin-ligase complex.
Hanna, J., Schütz, A., Zimmermann, F., Behlke, J., Sommer, T. and Heinemann, U.
Journal of Biological Chemistry 287 (11): 8633-8640. 9 March 2012

The SNF2-like helicase HELLS mediates E2F3-dependent transcription and cellular transformation.
von Eyss, B., Maaskola, J., Memczak, S., Möllmann, K., Schuetz, A., Loddenkemper, C., Tanh, M.D., Otto, A., Muegge, K., Heinemann, U., Rajewsky, N. and Ziebold, U.
EMBO Journal 31 (4): 972-985. 15 February 2012

The structure of the Klf4 DNA-binding domain links to self-renewal and macrophage differentiation.
Schuetz, A., Nana, D., Rose, C., Zocher, G., Milanovic, M., Koenigsmann, J., Blasig, R., Heinemann, U. and Carstanjen, D.
Cellular and Molecular Life Sciences 68 (18): 3121-3131. September 2011

Expression of protein complexes using multiple E. coli protein co-expression systems: a benchmarking study.
Busso, D., Peleg, Y., Heidebrecht, T., Romier, C., Jacobovitch, Y., Dantes, A., Salim, L., Troesch, E., Schuetz, A., Heinemann, U., Folkers, G.E., Geerlof, A., Wilmanns, M., Polewacz, A., Quedenau, C., Büssow, K., Adamson, R., Blagova, E., Walton, J., Cartwright, J.L., Bird, L.E., Owens, R.J., Berrow, N.S., Wilson, K.S., Sussman, J.L., Perrakis, A. and Celie, P.H.
Journal of Structural Biology 175 (2): 159-170. August 2011

Repression of transcriptional activity of C/EBPα by E2F-DP complexes.
Zaragoza, K., Bégay, V., Schuetz, A., Heinemann, U. and Leutz, A.
Molecular and Cellular Biology 30 (9): 2293-2304. May 2010

Usa1 functions as a scaffold of the HRD-ubiquitin ligase.
Horn, S.C., Hanna, J., Hirsch, C., Volkwein, C., Schütz, A., Heinemann, U., Sommer, T. and Jarosch, E.
Molecular Cell 36 (5): 782-793. 11 December 2009

Clinical variability in distal spinal muscular atrophy type 1 (DSMA1): determination of steady-state IGHMBP2 protein levels in five patients with infantile and juvenile disease.
Guenther, U.P., Handoko, L., Varon, R., Stephani, U., Tsao, C.Y., Mendell, J.R., Lützkendorf, S., Hübner, C., von Au, K., Jablonka, S., Dittmar, G., Heinemann, U., Schuetz, A. and Schuelke, M.
Journal of Molecular Medicine 87 (1): 31-41. January 2009

Patent

Xanthine derivatives, their use as a medicament, and pharmaceutical preparations comprising the same.
Max-Delbrück-Centrum für Molekulare Medizin.
201615552556. 22 February 2018

Xanthine derivatives, their use as a medicament, and pharmaceutical preparations comprising the same.
Max-Delbrück-Centrum für Molekulare Medizin.
WO/2016/135199. 1 September 2016

Review

A concise guide to choosing suitable gene expression systems for recombinant protein production.
Schütz, A., Bernhard, F., Berrow, N., Buyel, J.F., Ferreira-da-Silva, F., Haustraete, J., van den Heuvel, J., Hoffmann, J.E., de Marco, A., Peleg, Y., Suppmann, S., Unger, T., Vanhoucke, M., Witt, S. and Remans, K.
STAR Protocols 4 (4): 102572. 15 December 2023

Structural features of tight-junction proteins.
Heinemann, U. and Schuetz, A.
International Journal of Molecular Sciences 20 (23): 6020. 29 November 2019

Protein production and purification.
Gräslund, S., Nordlund, P., Weigelt, J., Hallberg, M., Bray, J., Gileadi, O., Knapp, S., Oppermann, U., Arrowsmith, C., Hui, R., Ming, J., dhe-Paganon, S., Park, H.W., Savchenko, A., Yee, A., Edwards, A., Vincentelli, R., Cambillau, C., Kim, R., Kim, S.H., Rao, Z., Shi, Y., Terwilliger, T.C., Kim, C.Y., Hung, L.W., Waldo, G.S., Peleg, Y., Albeck, S., Unger, T., Dym, O., Prilusky, J., Sussman, J.L., Stevens, R.C., Lesley, S.A., Wilson, I.A., Joachimiak, A., Collart, F., Dementieva, I., Donnelly, M.I., Eschenfeldt, W.H., Kim, Y.M., Stols, L., Wu, R., Zhou, M., Burley, S.K., Emtage, J.S., Sauder, J.M., Thompson, D., Bain, K., Luz, J., Gheyi, T., Zhang, F., Atwell, S., Almo, S.C., Bonanno, J.B., Fiser, A., Swaminathan, S., Studier, F.W., Chance, M.R., Sali, A., Acton, T.B., Xiao, R., Zhao, L., Ma, L.C., Hunt, J.F., Tong, L., Cunningham, K., Inouye, M., Anderson, S., Janjua, H., Shastry, R., Ho, C.K., Wang, D., Wang, H., Jiang, M., Montelione, G.T., Stuart, D.I., Owens, R.J., Daenke, S., Schütz, A., Heinemann, U., Yokoyama, S., Büssow, K. and Gunsalus, K.C.
Nature Methods 5 (2): 135-146. February 2008