Browse by Research Team

2024

ADAM19 cleaves the PTH receptor and associates with brachydactyly type E.
Aydin, A. and Klenk, C. and Nemec, K. and Işbilir, Ali and Martin, L.M. and Zauber, H. and Rrustemi, T. and Toka, H.R. and Schuster, H. and Gong, M. and Stricker, S. and Bock, A. and Bähring, S. and Selbach, M. and Lohse, M.J. and Luft, F.C.
Life Science Alliance 7 (4): e202302400. April 2024

Impairment of the adrenergic reserve associated with exercise intolerance in a murine model of heart failure with preserved ejection fraction.
Semmler, L. and Jeising, T. and Huettemeister, J. and Bathe-Peters, M. and Georgoula, K. and Roshanbin, R. and Sander, P. and Fu, S. and Bode, D. and Hohendanner, F. and Pieske, B. and Annibale, P. and Schiattarella, G.G. and Oeing, C.U. and Heinzel, F.R.
Acta Physiologica 240 (4): e14124. April 2024

Fluorescent tools for the imaging of dopamine D2-like receptors.
Nagl, M. and Moennich, D. and Rosier, N. and Schihada, H. and Sirbu, A. and Konar, N. and Reyes-Resina, I. and Navarro, G. and Franco, R. and Kolb, P. and Annibale, P. and Pockes, S.
ChemBioChem 25 (2): e202300659. 15 January 2024

Shedding light on the D1‐like receptors: a fluorescence‐based toolbox for visualization of the D1 and D5 receptors.
Rosier, N. and Moennich, D. and Nagl, M. and Schihada, H. and Sirbu, A. and Konar, N. and Reyes-Resina, I. and Navarro, G. and Franco, R. and Kolb, P. and Annibale, P. and Pockes, S.
ChemBioChem 25 (2): e202300658. 15 January 2024

2023

Quantitative morphological analysis of the T-tubular network of ventricular cardiomyocytes using novel image processing tools.
Georgoula, K. and Sage, D. and Annibale, P.
In: European Conferences on Biomedical Optics 2023, 25-29 June 2023, Munich, Germany. 11 August 2023

Spatiotemporal GPCR signaling illuminated by genetically encoded fluorescent biosensors.
Kayser, C. and Melkes, B. and Derieux, C. and Bock, A.
Current Opinion in Pharmacology 71 : 102384. August 2023

2022

Evaluation of pharmacological rescue of melanocortin-4 receptor nonsense mutations by aminoglycoside.
Höpfner, F. and Paisdzior, S. and Reininghaus, N. and Sohail, I. and Scheerer, P. and Annibale, P. and Biebermann, H. and Kühnen, P.
Life 12 (11): 1793. 5 November 2022

Fluorescence spectroscopy of low-level endogenous ß-adrenergic receptor expression at the plasma membrane of differentiating human iPSC-derived cardiomyocytes.
Gmach, P. and Bathe-Peters, M. and Telugu, N. and Miller, D.C. and Annibale, P.
International Journal of Molecular Sciences 23 (18): 10405. 8 September 2022

Development of an indole-amide-based photoswitchable cannabinoid receptor subtype 1 (CB(1)R) "Cis-On" agonist.
Rodríguez-Soacha, D.A. and Steinmüller, S.A.M. and Işbilir, A. and Fender, J. and Deventer, M.H. and Ramírez, Y.A. and Tutov, A. and Sotriffer, C. and Stove, C.P. and Lorenz, K. and Lohse, M.J. and Hislop, J.N. and Decker, M.
ACS Chemical Neuroscience 13 (16): 2410-2435. 17 August 2022

Functional modulation of PTH1R activation and signaling by RAMP2.
Nemec, K. and Schihada, H. and Kleinau, G. and Zabel, U. and Grushevsky, E.O. and Scheerer, P. and Lohse, M.J. and Maiellaro, I.
Proceedings of the National Academy of Sciences of the United States of America 119 (32): e2122037119. 9 August 2022

The impact of membrane protein diffusion on GPCR signaling.
Boltz, H.H. and Sirbu, A. and Stelzer, N. and de Lanerolle, P. and Winkelmann, S. and Annibale, P.
Cells 11 (10): 1660. 17 May 2022

Receptor-associated independent cAMP nanodomains mediate spatiotemporal specificity of GPCR signaling.
Anton, S.E. and Kayser, C. and Maiellaro, I. and Nemec, K. and Möller, J. and Koschinski, A. and Zaccolo, M. and Annibale, P. and Falcke, M. and Lohse, M.J. and Bock, A.
Cell 185 (7): 1130-1142.e11. 31 March 2022

Unraveling the hidden temporal range of fast β(2)-adrenergic receptor mobility by time-resolved fluorescence.
Balakrishnan, A. and Hemmen, K. and Choudhury, S. and Krohn, J.H. and Jansen, K. and Friedrich, M. and Beliu, G. and Sauer, M. and Lohse, M.J. and Heinze, K.G.
Communications Biology 5 (1): 176. 28 February 2022

One gene - two proteins: the C-terminus of the prototypical M2 muscarinic receptor localizes to the mitochondria.
Fasciani, I. and Petragnano, F. and Wang, Z. and Edwards, R. and Telugu, N. and Pietrantoni, I. and Zauber, H. and Rossi, M. and Zabel, U. and Grieben, M. and Terzenidou, M.E. and Di Gregorio, J. and Pellegrini, C. and Santini, S. and Taddei, A.R. and Poehl, B. and Aringhieri, S. and Aloisi, G. and Marampon, F. and Roman, A. and Diecke, S. and Flati, V. and Giorgi, F. and Amicarelli, F. and Tobin, A.B. and Scarselli, M. and Tokatlidis, K. and Lohse, M.J. and Maggio, R. and Annibale, P.
bioRxiv : 2021.04.13.438299. 26 February 2022

Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone 1 receptor.
Klenk, C. and Hommers, L. and Lohse, M.J.
Frontiers in Endocrinology 13 : 839351. 22 February 2022

Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1.
Klenk, C. and Hommers, L. and Lohse, M.J.
bioRxiv : 2021.12.16.472984. 19 January 2022

Real-time measurements of intracellular cAMP gradients using FRET-based cAMP nanorulers.
Kayser, C. and Lohse, M.J. and Bock, A.
Methods in Molecular Biology 2483 : 1-13. 2022

2021

Dual-color fluorescence cross-correlation spectroscopy to study protein-protein interaction and protein dynamics in live cells.
Hemmen, K. and Choudhury, S. and Friedrich, M. and Balkenhol, J. and Knote, F. and Lohse, M.J. and Heinze, K.G.
Journal of Visualized Experiments 178 : e62954. 11 December 2021

Viewpoints on the first transatlantic GPCR symposium for early-career investigators.
Bender, B.J. and Bock, A. and Nesheva, D.N. and Perry-Hauser, N.A.
ACS Pharmacology & Translational Science 4 (5): 1705-1711. 8 October 2021

Dual-color fluorescence cross-correlation spectroscopy to study protein-protein interaction and protein dynamics in live cells.
Hemmen, K. and Choudhury, S. and Friedrich, M. and Balkenhol, J. and Knote, F. and Lohse, M. and Heinze, K.G.
bioRxiv : 2021.09.10.459760v1. 12 September 2021

A versatile sub-nanomolar fluorescent ligand enables NanoBRET binding studies and single-molecule microscopy at the histamine H(3) receptor.
Rosier, N. and Grätz, L. and Schihada, H. and Möller, J. and Işbilir, A. and Humphrys, L.J. and Nagl, M. and Seibel, U. and Lohse, M.J. and Pockes, S.
Journal of Medicinal Chemistry 64 (15): 11695-11708. 12 August 2021

Visualization of β-adrenergic receptor dynamics and differential localization in cardiomyocytes.
Bathe-Peters, M. and Gmach, P. and Boltz, H.H. and Einsiedel, J. and Gotthardt, M. and Hübner, H. and Gmeiner, P. and Lohse, M.J. and Annibale, P.
Proceedings of the National Academy of Sciences of the United States of America 118 (23): e2101119118. 8 June 2021

Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors.
Thibado, J.K and Tano, J.Y. and Lee, J. and Salas-Estrada, L. and Provasi, D. and Strauss, A. and Ribeiro, J.M.L. and Xiang, G. and Broichhagen, J. and Filizola, M. and Lohse, M.J. and Levitz, J.
eLife 10 : e67027. 21 April 2021

Quantitative spectroscopy of single molecule interaction times.
Boltz, H.H. and Sirbu, A. and Stelzer, N. and Lohse, M.J. and Schütte, C. and Annibale, P.
Optics Letters 46 (7): 1538-1541. 1 April 2021

Allosteric coupling and biased agonism in G protein-coupled receptors.
Bock, A. and Bermudez, M.
FEBS Journal 288 (8): 2513-2528. April 2021

Atypical antipsychotics and metabolic syndrome: from molecular mechanisms to clinical differences.
Carli, M. and Kolachalam, S. and Longoni, B. and Pintaudi, A. and Baldini, M. and Aringhieri, S. and Fasciani, I. and Annibale, P. and Maggio, R. and Scarselli, M.
Pharmaceuticals 14 (3): 238. March 2021

Determination of G-protein-coupled receptor oligomerization by molecular brightness analyses in single cells.
Işbilir, A. and Serfling, R. and Möller, J. and Thomas, R. and De Faveri, C. and Zabel, U. and Scarselli, M. and Beck-Sickinger, A.G. and Bock, A. and Coin, I. and Lohse, M.J. and Annibale, P.
Nature Protocols 16 (3): 1419-1451. March 2021

Structural and functional characterization of allatostatin receptor type-C of Thaumetopoea pityocampa, a potential target for next-generation pest control agents.
Shahraki, A. and Işbilir, A. and Dogan, B. and Lohse, M.J. and Durdagi, S. and Birgul-Iyison, N.
Journal of Chemical Information and Modeling 61 (2): 715-728. 22 February 2021

Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors.
Thibado, J. and Tano, J.Y. and Lee, J. and Salas-Estrada, L. and Provasi, D. and Strauss, A. and Lamim Ribeiro, J.M. and Xiang, G. and Broichhagen, J. and Filizola, M. and Lohse, M. and Levitz, J.
bioRxiv : 2021.02.04.429701. 6 February 2021

Bioluminescence in G protein-coupled receptors drug screening using nanoluciferase and halo-tag technology.
Schihada, H. and Nemec, K. and Lohse, M.J. and Maiellaro, I.
Methods in Molecular Biology 2268 : 137-147. 2021

2020

Pharmacological characterization of the stick insect Carausius morosus allatostatin-C receptor with its endogenous agonist.
Işbilir, A. and Duan Sahbaz, B. and Tuncgenc, G. and Bünemann, M. and Lohse, M.J. and Birgül-Iyison, N.
ACS Omega 5 (50): 32183-32194. 22 December 2020

The role of orthosteric building blocks of bitopic ligands for muscarinic M1 receptors.
Volpato, D. and Kauk, M. and Messerer, R. and Bermudez, M. and Wolber, G. and Bock, A. and Hoffmann, C. and Holzgrabe, U.
ACS Omega 5 (49): 31706-31715. 15 December 2020

Advanced fluorescence microscopy reveals disruption of dynamic CXCR4 dimerization by subpocket-specific inverse agonists.
Işbilir, A. and Möller, J. and Arimont, M. and Bobkov, V. and Perpiñá-Viciano, C. and Hoffmann, C. and Inoue, A. and Heukers, R. and de Graaf, C. and Smit, M.J. and Annibale, P. and Lohse, M.J.
Proceedings of the National Academy of Sciences of the United States of America 117 (46): 29144-29154. 17 November 2020

Ligand-specific allosteric coupling controls G-protein-coupled receptor signaling.
Holze, J. and Bermudez, M. and Pfeil, E.M. and Kauk, M. and Bödefeld, T. and Irmen, M. and Matera, C. and Dallanoce, C. and De Amici, M. and Holzgrabe, U. and König, G.M. and Tränkle, C. and Wolber, G. and Schrage, R. and Mohr, K. and Hoffmann, C. and Kostenis, E. and Bock, A.
ACS Pharmacology & Translational Science 3 (5): 859-867. 9 October 2020

Optical mapping of cAMP signaling at the nanometer scale.
Bock, A. and Annibale, P. and Konrad, C. and Hannawacker, A. and Anton, S.E. and Maiellaro, I. and Zabel, U. and Sivaramakrishnan, S. and Falcke, M. and Lohse, M.J.
Cell 182 (6): 1519-1530. 17 September 2020

Single-molecule analysis reveals agonist-specific dimer formation of µ-opioid receptors.
Möller, J. and Isbilir, A. and Sungkaworn, T. and Osberg, B. and Karathanasis, C. and Sunkara, V. and Grushevskyi, E.O. and Bock, A. and Annibale, P. and Heilemann, M. and Schütte, C. and Lohse, M.J.
Nature Chemical Biology 16 (9): 946-954. September 2020

Establishing a sensitive fluorescence-based quantification method for cyclic nucleotides.
Gruteser, N. and Kohlhas, V. and Balfanz, S. and Franzen, A. and Günther, A. and Offenhäusser, A. and Müller, F. and Nikolaev, V. and Lohse, M.J. and Baumann, A.
BMC Biotechnology 20 (1): 47. 27 August 2020

Kinetic analysis of the early signaling steps of the human chemokine receptor CXCR4.
Perpina-Viciano, C. and Işbilir, A. and Zarca, A. and Caspar, B. and Kilpatrick, L.E. and Hill, S.J. and Smit, M.J. and Lohse, M.J. and Hoffmann, C.
Molecular Pharmacology 98 (2): 72-87. August 2020

Unmasking features of the auto-epitope essential for β(1)-adrenoceptor activation by autoantibodies in chronic heart failure.
Wölfel, A. and Sättele, M. and Zechmeister, C. and Nikolaev, V.O. and Lohse, M.J. and Boege, F. and Jahns, R. and Boivin-Jahns, V.
ESC Heart Failure 7 (4): 1830-1841. August 2020

Structural and functional characterization of allatostatin receptor type-C of Thaumetopoea pityocampa revealed the importance of Q271(6.55) residue in G protein-dependent activation pathway.
Shahraki, A. and Isbilir, A. and Dogan, B. and Lohse, M.J. and Durdagi, S. and Birgul-Iyison, N.
bioRxiv : 2020.07.01.174037. 2 July 2020

Development of a conformational histamine H(3) receptor biosensor for the synchronous screening of agonists and inverse agonists.
Schihada, H. and Ma, X. and Zabel, U. and Vischer, H.F. and Schulte, G. and Leurs, R. and Pockes, S. and Lohse, M.J.
ACS Sensors 5 (6): 1734-1742. 26 June 2020

Visualizing the functional 3D shape and topography of long noncoding RNAs by single-particle atomic force microscopy and in-solution hydrodynamic techniques.
Uroda, T. and Chillón, I. and Annibale, P. and Teulon, J.M. and Pessey, O. and Karuppasamy, M. and Pellequer, J.L. and Marcia, M.
Nature Protocols 15 (6): 2107-2139. June 2020

Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope.
Bathe-Peters, M. and Gmach, P. and Annibale, P. and Lohse, M.J.
Data in Brief 29 : 105063. April 2020

Spatial heterogeneity in molecular brightness.
Annibale, P. and Lohse, M.J.
Nature Methods 17 (3): 273-275. March 2020

Differential signaling profiles of MC4R mutations with three different ligands.
Paisdzior, S. and Dimitriou, I.M. and Schöpe, P.C. and Annibale, P. and Scheerer, P. and Krude, H. and Lohse, M.J. and Biebermann, H. and Kühnen, P.
International Journal of Molecular Sciences 21 (4): 1224. 12 February 2020

Hypnotika und Sedativa.
Lohse, M.J.
In: Arzneiverordnungs-Report 2020 : Aktuelle Daten, Kosten, Trends und Kommentare. Springer, Berlin, Heidelberg, 571-582. ISBN 978-3-662-62167-7 2020

Ophthalmika.
Lohse, M.J.
In: Arzneiverordnungs-Report 2020 : Aktuelle Daten, Kosten, Trends und Kommentare. Springer, Berlin, Heidelberg, 733-758. ISBN 978-3-662-62168-4 2020

Psychopharmaka.
Lohse, M.J.
In: Arzneiverordnungs-Report 2020 : Aktuelle Daten, Kosten, Trends und Kommentare. Springer, Berlin, Heidelberg, 781-814. ISBN 978-3-662-62168-4 2020

2019

Context-dependent signaling of CXC chemokine receptor 4 and atypical chemokine receptor 3.
Heuninck, J. and Perpina Viciano, C. and Işbilir, A. and Caspar, B. and Capoferri, D. and Briddon, S.J. and Durroux, T. and Hill, S.J. and Lohse, M.J. and Milligan, G. and Pin, J.P. and Hoffmann, C.
Molecular Pharmacology 96 (6): 778-793. December 2019

Vibrational spectroscopy as a powerful tool for follow-up immunoadsorption therapy treatment of dilated cardiomyopathy - a case report.
Huang, J. and Ramoji, A. and Guo, S. and Bocklitz, T. and Boivin-Jahns, V. and Möller, J. and Kiehntopf, M. and Noutsias, M. and Popp, J. and Neugebauer, U.
Analyst 145 (2): 486-496. 15 November 2019

Conserved pseudoknots in lncRNA MEG3 are essential for stimulation of the p53 pathway.
Uroda, T. and Anastasakou, E. and Rossi, A. and Teulon, J.M. and Pellequer, J.L. and Annibale, P. and Pessey, O. and Inga, A. and Chillón, I. and Marcia, M.
Molecular Cell 75 (5): 982-995. 5 September 2019

Quantitative single-residue bioorthogonal labeling of G protein-coupled receptors in live cells.
Serfling, R. and Seidel, L. and Bock, A. and Lohse, M.J. and Annibale, P. and Coin, I.
ACS Chemical Biology 14 (6): 1141-1149. 21 June 2019

Stepwise activation of a class C GPCR begins with millisecond dimer rearrangement.
Grushevskyi, E.O. and Kukaj, T. and Schmauder, R. and Bock, A. and Zabel, U. and Schwabe, T. and Benndorf, K. and Lohse, M.J.
Proceedings of the National Academy of Sciences of the United States of America 116 (20): 10150-10155. 14 May 2019

Does divergent binding pocket closure drive ligand bias for class A GPCRs?
Bermudez, M. and Bock, A.
Trends in Pharmacological Sciences 40 (4): 236-239. April 2019

All-in-one confocal and widefield fluorescence microscope to investigate membrane receptor dynamics.
Bathe-Peters, M. and Gmach, P. and Annibale, P. and Lohse, M.J.
In: Imaging and Applied Optics Congress 2019 (COSI, IS, MATH, pcAOP), 24-27 Jun 2019, Munich, Germany. 2019

2018

A functional genetic variation of SLC6A2 repressor hsa-miR-579-3p upregulates sympathetic noradrenergic processes of fear and anxiety.
Hommers, L.G. and Richter, J. and Yang, Y. and Raab, A. and Baumann, C. and Lang, K. and Schiele, M.A. and Weber, H. and Wittmann, A. and Wolf, C. and Alpers, G.W. and Arolt, V. and Domschke, K. and Fehm, L. and Fydrich, T. and Gerlach, A. and Gloster, A.T. and Hamm, A.O. and Helbig-Lang, S. and Kircher, T. and Lang, T. and Pané-Farré, C.A. and Pauli, P. and Pfleiderer, B. and Reif, A. and Romanos, M. and Straube, B. and Ströhle, A. and Wittchen, H.U. and Frantz, S. and Ertl, G. and Lohse, M.J. and Lueken, U. and Deckert, J.
Translational Psychiatry 8 (1): 226. 19 October 2018

A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics.
Schihada, H. and Vandenabeele, S. and Zabel, U. and Frank, M. and Lohse, M.J. and Maiellaro, I.
Communications Biology 1 : 105. 7 August 2018

Allosteric modulators targeting CNS muscarinic receptors.
Bock, A. and Schrage, R. and Mohr, K.
Neuropharmacology 136 (Part C): 427-437. 1 July 2018

A panoramic view on GPCRs: the 1st Berlin Symposium for Interdisciplinary GPCR research.
Bock, A. and Bermudez, M.
Naunyn-Schmiedeberg's Archives of Pharmacology 391 (7): 769-771. July 2018

Molecular details of dimerization kinetics reveal negligible populations of transient µ-opioid receptor homodimers at physiological concentrations.
Meral, D. and Provasi, D. and Prada-Gracia, D. and Möller, J. and Marino, K. and Lohse, M.J. and Filizola, M.
Scientific Reports 8 (1): 7705. 16 May 2018

Increased fear learning, spatial learning as well as neophobia in Rgs2(-/-) mice.
Raab, A. and Popp, S. and Lesch, K.P. and Lohse, M.J. and Fischer, M. and Deckert, J. and Hommers, L.
Genes Brain and Behavior 17 (4): e12420. April 2018

All-optical microscope autofocus based on an electrically tunable lens and a totally internally reflected IR laser.
Bathe-Peters, M. and Annibale, P. and Lohse, M.J.
Optics Express 26 (3): 2359-2368. 5 February 2018

Related GPCRs couple differently to G(s): preassociation between G protein and 5-HT(7) serotonin receptor reveals movement of Gα(s) upon receptor activation.
Andressen, K.W. and Ulsund, A.H. and Krobert, K.A. and Lohse, M.J. and Buenemann, M. and Levy, F.O.
FASEB Journal 32 (2): 1059-1069. 5 February 2018

2017

Visualization of class A GPCR oligomerization by image-based fluorescence fluctuation spectroscopy.
Isbilir, A. and Moeller, J. and Bock, A. and Zabel, U. and Annibale, P. and Lohse, M.J.
bioRxiv : 240903. 29 December 2017

Evolutionary action and structural basis of the allosteric switch controlling β(2)AR functional selectivity.
Schönegge, A.M. and Gallion, J. and Picard, L.P. and Wilkins, A.D. and Le Gouill, C. and Audet, M. and Stallaert, W. and Lohse, M.J. and Kimmel, M. and Lichtarge, O. and Bouvier, M.
Nature Communications 8 (1): 2169. 18 December 2017

Single-molecule imaging reveals receptor-G protein interactions at cell surface hot spots.
Sungkaworn, T. and Jobin, M.L. and Burnecki, K. and Weron, A. and Lohse, M.J. and Calebiro, D.
Nature 550 (7677): 543-547. 26 October 2017

Mapping the dynamics of the glucocorticoid receptor within the nuclear landscape.
Stortz, M. and Presman, D.M. and Bruno, L. and Annibale, P. and Dansey, M.V. and Burton, G. and Gratton, E. and Pecci, A. and Levi, V.
Scientific Reports 7 (1): 6219. 24 July 2017

Ligand-specific restriction of extracellular conformational dynamics constrains signaling of the M(2) muscarinic receptor.
Bermudez, M. and Bock, A. and Krebs, F. and Holzgrabe, U. and Mohr, K. and Lohse, M.J. and Wolber, G.
ACS Chemical Biology 12 (7): 1743-1748. 21 July 2017

Is signaling specificity encoded in arrestin conformation?
Hoffmann, C. and Lohse, M.J. and Luttrell, L.M.
In: The Structural Basis of Arrestin Functions. Springer, Cham, Switzerland, 235-253. ISBN 978-3-319-57552-0 25 May 2017

Was ist in der Medizin moeglich: Neue Therapien.
Lohse, M.
In: Medizin 4.0 - Zur Zukunft der digitalisierten Welt. IV. Interdisziplinaerer Kongress Berlin 2016 mit der Heinz Nixdorf-Stiftung - gemeinsam mit der Charite - Universitaetsmedizin Berlin, 1-2 June 2016, Berlin, Germany. 2017