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| Item Type: | Article |
|---|---|
| Title: | TIFA renders intestinal epithelial cells responsive to microbial ADP-heptose and drives colonic inflammation in mice |
| Creators Name: | Erkert, L., Ruder, B., Kabisch, M., Gamez Belmonte, R., Patankar, J.V., Gonzalez Acera, M., Schödel, L., Chiriac, M.T., Cineus, R., Gnafakis, S., Leupold, T., Thoma, O.M., Stolzer, I., Taut, A., Thonn, V., Zundler, S., Günther, C., Diefenbach, A., Kühl, A.A., Hegazy, A.N., Waldner, M., Basic, M., Bleich, A., Neurath, M.F., Wirtz, S. and Becker, C. |
| Abstract: | Intestinal immune homeostasis relies on intestinal epithelial cells (IECs), which provide an efficient barrier, and warrant a state of tolerance between the microbiome and the mucosal immune system. Thus, proper epithelial microbial sensing and handling of microbes is key to preventing excessive immunity, such as seen in patients with inflammatory bowel disease (IBD). To date, the molecular underpinnings of these processes remain incompletely understood. This study identifies TIFA as a driver of intestinal inflammation and an epithelial signaling hub between the microbiome and mucosal immune cells. TIFA was constitutively expressed in crypt epithelial cells and was highly induced in the intestine of mice and IBD patients with intestinal inflammation. We further identified IL-22 signaling via STAT3 as key mechanism driving TIFA expression in IECs. At the molecular level, we demonstrate that TIFA expression is essential for IEC responsiveness to the bacterial metabolite ADP-heptose. Most importantly, ADP-heptose-induced TIFA signaling orchestrates an inflammatory cellular response in the epithelium, with NF-κB and inflammasome activation, and high levels of chemokine production. Finally, mice lacking TIFA were protected from intestinal inflammation when subjected to a model of experimental colitis. In conclusion, our study implicates that targeting TIFA may be a strategy for future IBD therapy. |
| Keywords: | TIFA, Intestinal Epithelium, IBD, ADP-Heptose, IL-22, Animals, Mice |
| Source: | Mucosal Immunology |
| ISSN: | 1933-0219 |
| Publisher: | Nature Publishing Group |
| Date: | 20 January 2025 |
| Additional Information: | Leif S.-H. Ludwig and Ashley Sanders are members of the TRR241 IBDome Consortium. |
| Official Publication: | https://doi.org/10.1016/j.mucimm.2025.01.003 |
| PubMed: | View item in PubMed |
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