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| Item Type: | Article |
|---|---|
| Title: | Hyperphenylalaninemia and serotonin deficiency in Dnajc12-deficient mice |
| Creators Name: | Cao, Y., Popp, O., Milani, N., Qadri, F., Kühn, R., Mertins, P., Bader, M. and Alenina, N. |
| Abstract: | Serotonin exerts numerous neurological and physiological actions in the brain and in the periphery. It is generated by two different tryptophan hydroxylase enzymes, TPH1 and TPH2, in the periphery and in the brain, respectively, which are members of the aromatic amino acid hydroxylase (AAAH) family together with phenylalanine hydroxylase (PAH), degrading phenylalanine, and tyrosine hydroxylase (TH), generating dopamine. In this study, we show that the co-chaperone DNAJC12 is downregulated in serotonergic neurons in the brain of mice lacking TPH2 and thereby central serotonin. DNAJC12 has been described to regulate the stability of PAH and mutations in its gene cause hyperphenylalaninemia and neurological symptoms in patients. We show that DNAJC12 also binds and stabilizes TPH1 and TPH2 in transfected cells. In order to clarify the importance of DNAJC12 in the regulation of neurotransmitter synthesis and phenylalanine degradation in vivo, we generated DNAJC12-deficient mice. These mice show reduced levels and activity of PAH, TPH2, and TPH1 in liver, brain, and pineal gland, respectively, and experience hyperphenylalaninemia and central and peripheral serotonin deficiency. These data support a pivotal role of DNAJC12 in the regulation of AAAH and thereby in neurotransmitter synthesis and phenylalanine homeostasis. |
| Keywords: | Brain, Inbred C57BL Mice, Knockout Mice, Molecular Chaperones, Phenylalanine Hydroxylase, Phenylketonurias, Serotonin, Tryptophan Hydroxylase, Animals, Mice |
| Source: | Communications Biology |
| ISSN: | 2399-3642 |
| Publisher: | Springer Nature |
| Volume: | 7 |
| Number: | 1 |
| Page Range: | 1641 |
| Date: | 18 December 2024 |
| Official Publication: | https://doi.org/10.1038/s42003-024-07360-6 |
| PubMed: | View item in PubMed |
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