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| Item Type: | Article |
|---|---|
| Title: | Subcellular mRNA kinetic modeling reveals nuclear retention as rate-limiting |
| Creators Name: | Steinbrecht, D., Minia, I., Milek, M., Meisig, J., Blüthgen, N. and Landthaler, M. |
| Abstract: | Eukaryotic mRNAs are transcribed, processed, translated, and degraded in different subcellular compartments. Here, we measured mRNA flow rates between subcellular compartments in mouse embryonic stem cells. By combining metabolic RNA labeling, biochemical fractionation, mRNA sequencing, and mathematical modeling, we determined the half-lives of nuclear pre-, nuclear mature, cytosolic, and membrane-associated mRNAs from over 9000 genes. In addition, we estimated transcript elongation rates. Many matured mRNAs have long nuclear half-lives, indicating nuclear retention as the rate-limiting step in the flow of mRNAs. In contrast, mRNA transcripts coding for transcription factors show fast kinetic rates, and in particular short nuclear half-lives. Differentially localized mRNAs have distinct rate constant combinations, implying modular regulation. Membrane stability is high for membrane-localized mRNA and cytosolic stability is high for cytosol-localized mRNA. mRNAs encoding target signals for membranes have low cytosolic and high membrane half-lives with minor differences between signals. Transcripts of nuclear-encoded mitochondrial proteins have long nuclear retention and cytoplasmic kinetics that do not reflect co-translational targeting. Our data and analyses provide a useful resource to study spatiotemporal gene expression regulation. |
| Keywords: | Kinetic Modeling, Metabolic Labeling, Nuclear Retention, RNA Dynamics, Subcellular Fractionation, Animals, Mice |
| Source: | Molecular Systems Biology |
| ISSN: | 1744-4292 |
| Publisher: | Nature Publishing Group |
| Volume: | 20 |
| Number: | 12 |
| Page Range: | 1346-1371 |
| Date: | 2 December 2024 |
| Official Publication: | https://doi.org/10.1038/s44320-024-00073-2 |
| PubMed: | View item in PubMed |
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