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MYC-containing amplicons in acute myeloid leukemia: genomic structures, evolution, and transcriptional consequences

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Item Type:Article
Title:MYC-containing amplicons in acute myeloid leukemia: genomic structures, evolution, and transcriptional consequences
Creators Name:L'Abbate, A., Tolomeo, D., Cifola, I., Severgnini, M., Turchiano, A., Augello, B., Squeo, G., D'Addabbo, P., Traversa, D., Daniele, G., Lonoce, A., Pafundi, M., Carella, M., Palumbo, O., Dolnik, A., Muehlematter, D., Schoumans, J., Van Roy, N., De Bellis, G., Martinelli, G., Merla, G., Bullinger, L., Haferlach, C. and Storlazzi, C.T.
Abstract:Double minutes (dmin), homogeneously staining regions, and ring chromosomes are vehicles of gene amplification in cancer. The underlying mechanism leading to their formation as well as their structure and function in acute myeloid leukemia (AML) remain mysterious. We combined a range of high-resolution genomic methods to investigate the architecture and expression pattern of amplicons involving chromosome band 8q24 in 23 cases of AML (AML-amp). This revealed that different MYC-dmin architectures can coexist within the same leukemic cell population, indicating a step-wise evolution rather than a single event origin, such as through chromothripsis. This was supported also by the analysis of the chromothripsis criteria, that poorly matched the model in our samples. Furthermore, we found that dmin could evolve toward ring chromosomes stabilized by neocentromeres. Surprisingly, amplified genes (mainly PVT1) frequently participated in fusion transcripts lacking a corresponding DNA template. We also detected a significant overexpression of the circular RNA of PVT1 (circPVT1) in AML-amp cases versus AML with a normal karyotype. Our results show that 8q24 amplicons in AML are surprisingly plastic DNA structures with an unexpected association to novel fusion transcripts and circular RNAs.
Keywords:Acute Myeloid Leukemia, Chromosome Aberrations, Chromosome Banding, Gene Amplification, Genetic Transcription, Genomics, Karyotyping, Long Noncoding RNA, myc Genes, Pair 8 Human Chromosomes
Source:Leukemia
ISSN:1476-5551
Publisher:Nature Publishing Group
Volume:32
Number:10
Page Range:2152-2166
Date:October 2018
Official Publication:https://doi.org/10.1038/s41375-018-0033-0
PubMed:View item in PubMed

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