Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status

Coscia, F.; Watters, K.M.; Curtis, M.; Eckert, M.A.; Chiang, C.Y.; Tyanova, S.; Montag, A.; Lastra, R.R.; Lengyel, E.; Mann, M.

Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status

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Item Type:	Article
Title:	Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status
Creators Name:	Coscia, F., Watters, K.M., Curtis, M., Eckert, M.A., Chiang, C.Y., Tyanova, S., Montag, A., Lastra, R.R., Lengyel, E. and Mann, M.
Abstract:	A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumour of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here, we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumours, immortalized ovarian surface epithelial cells and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantification of >10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II) and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic data set, as well as a confirmatory publicly available CPTAC/TCGA tumour proteome data set, into a predominantly epithelial and mesenchymal HGSOC tumour cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumours indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.
Keywords:	Datasets as Topic, Epithelial Cells, Fallopian Tubes, Gene Expression Profiling, Mass Spectrometry, Neoplasm Grading, Ovarian Neoplasms, Ovary, Primary Cell Culture, Proteomics, Transcriptome, Tumor Cell Line
Source:	Nature Communications
ISSN:	2041-1723
Publisher:	Nature Publishing Group
Volume:	7
Page Range:	12645
Date:	26 August 2016
Official Publication:	https://doi.org/10.1038/ncomms12645
PubMed:	View item in PubMed

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