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| Item Type: | Article |
|---|---|
| Title: | Tracing tumorigenesis in a solid tumor model at single-cell resolution |
| Creators: |
Praktiknjo, S.D.  ORCID: https://orcid.org/0000-0002-0186-5271, Obermayer, B. ORCID: https://orcid.org/0000-0002-9116-630X, Zhu, Q., Fang, L., Liu, H. ORCID: https://orcid.org/0009-0007-4871-452X, Quinn, H. ORCID: https://orcid.org/0000-0002-1336-5709, Stoeckius, M. ORCID: https://orcid.org/0000-0002-5658-029X, Kocks, C. ORCID: https://orcid.org/0000-0003-1749-3334, Birchmeier, W. ORCID: https://orcid.org/0000-0003-1173-0829 and Rajewsky, N. ORCID: https://orcid.org/0000-0002-4785-4332
|
| Abstract: | Characterizing the complex composition of solid tumors is fundamental for understanding tumor initiation, progression and metastasis. While patient-derived samples provide valuable insight, they are heterogeneous on multiple molecular levels, and often originate from advanced tumor stages. Here, we use single-cell transcriptome and epitope profiling together with pathway and lineage analyses to study tumorigenesis from a developmental perspective in a mouse model of salivary gland squamous cell carcinoma. We provide a comprehensive cell atlas and characterize tumor-specific cells. We find that these cells are connected along a reproducible developmental trajectory: initiated in basal cells exhibiting an epithelial-to-mesenchymal transition signature, tumorigenesis proceeds through Wnt-differential cancer stem cell-like subpopulations before differentiating into luminal-like cells. Our work provides unbiased insights into tumor-specific cellular identities in a whole tissue environment, and emphasizes the power of using defined genetic model systems. |
| Keywords: | Carcinogenesis, Squamous Cell Carcinoma, Cell Differentiation, Cell Lineage, Neoplastic Cell Transformation, Epithelial-Mesenchymal Transition, Gene Expression Profiling, Inbred C57BL Mice, Transgenic Mice, Neoplastic Stem Cells, Messenger RNA, RNA-Seq, Salivary Gland Neoplasms, Single-Cell Analysis, Tumor Microenvironment, Animals, Mice |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 11 |
| Number: | 1 |
| Page Range: | 991 |
| Date: | 20 February 2020 |
| Official Publication: | https://doi.org/10.1038/s41467-020-14777-0 |
| PubMed: | View item in PubMed |
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