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Single-cell RNA-seq: advances and future challenges

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Item Type:Article
Title:Single-cell RNA-seq: advances and future challenges
Creators Name:Saliba, A.E. and Westermann, A.J. and Gorski, S.A. and Vogel, J.
Abstract:Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNA-seq protocols have been introduced and are reviewed here-each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field.
Keywords:Cell Separation, Gene Expression Profiling, High-Throughput Nucleotide Sequencing, RNA Sequence Analysis, Single-Cell Analysis
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:8845-8860
Date:18 August 2014
Official Publication:https://doi.org/10.1093/nar/gku555
PubMed:View item in PubMed

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