Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Single-cell RNA-seq: advances and future challenges

[thumbnail of Original Article]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB

Item Type:Article
Title:Single-cell RNA-seq: advances and future challenges
Creators Name:Saliba, A.E., Westermann, A.J., Gorski, S.A. and Vogel, J.
Abstract:Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNA-seq protocols have been introduced and are reviewed here-each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field.
Keywords:Cell Separation, Gene Expression Profiling, High-Throughput Nucleotide Sequencing, RNA Sequence Analysis, Single-Cell Analysis
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press
Volume:42
Number:14
Page Range:8845-8860
Date:18 August 2014
Official Publication:https://doi.org/10.1093/nar/gku555
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library