Single-cell RNA-seq: advances and future challenges

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Item Type:	Article
Title:	Single-cell RNA-seq: advances and future challenges
Creators Name:	Saliba, A.E., Westermann, A.J., Gorski, S.A. and Vogel, J.
Abstract:	Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNA-seq protocols have been introduced and are reviewed here-each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field.
Keywords:	Cell Separation, Gene Expression Profiling, High-Throughput Nucleotide Sequencing, RNA Sequence Analysis, Single-Cell Analysis
Source:	Nucleic Acids Research
ISSN:	0305-1048
Publisher:	Oxford University Press
Volume:	42
Number:	14
Page Range:	8845-8860
Date:	18 August 2014
Official Publication:	https://doi.org/10.1093/nar/gku555
PubMed:	View item in PubMed

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