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Bacterial Vipp1 and PspA are members of the ancient ESCRT-III membrane-remodeling superfamily

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Item Type:Article
Title:Bacterial Vipp1 and PspA are members of the ancient ESCRT-III membrane-remodeling superfamily
Creators Name:Liu, J. and Tassinari, M. and Souza, D.P. and Naskar, S. and Noel, J.K. and Bohuszewicz, O. and Buck, M. and Williams, T.A. and Baum, B. and Low, H.H.
Abstract:Membrane remodeling and repair are essential for all cells. Proteins that perform these functions include Vipp1/IM30 in photosynthetic plastids, PspA in bacteria, and ESCRT-III in eukaryotes. Here, using a combination of evolutionary and structural analyses, we show that these protein families are homologous and share a common ancient evolutionary origin that likely predates the last universal common ancestor. This homology is evident in cryo-electron microscopy structures of Vipp1 rings from the cyanobacterium Nostoc punctiforme presented over a range of symmetries. Each ring is assembled from rungs that stack and progressively tilt to form dome-shaped curvature. Assembly is facilitated by hinges in the Vipp1 monomer, similar to those in ESCRT-III proteins, which allow the formation of flexible polymers. Rings have an inner lumen that is able to bind and deform membranes. Collectively, these data suggest conserved mechanistic principles that underlie Vipp1, PspA, and ESCRT-III-dependent membrane remodeling across all domains of life.
Keywords:Vipp1/IM30, PspA, ESCRT-III, Membrane Remodeling, Cryoelectron Microscopy, Ring Structure, Cytoskeleton, LUCA, Evolution, Eukaryogenesis
Source:Cell
ISSN:0092-8674
Publisher:Cell Press
Volume:184
Number:14
Page Range:3660-3673
Date:8 July 2021
Official Publication:https://doi.org/10.1016/j.cell.2021.05.041
PubMed:View item in PubMed

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