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| Item Type: | Article |
|---|---|
| Title: | The chloroplast RNA binding protein CP31A has a preference for mRNAs encoding the subunits of the chloroplast NAD(P)H dehydrogenase complex and is required for their accumulation |
| Creators Name: | Lenzen, B. and Rühle, T. and Lehniger, M.K. and Okuzaki, A. and Labs, M. and Muino, J.M. and Ohler, U. and Leister, D. and Schmitz-Linneweber, C. |
| Abstract: | Chloroplast RNA processing requires a large number of nuclear-encoded RNA binding proteins (RBPs) that are imported post-translationally into the organelle. Most of these RBPs are highly specific for one or few target RNAs. By contrast, members of the chloroplast ribonucleoprotein family (cpRNPs) have a wider RNA target range. We here present a quantitative analysis of RNA targets of the cpRNP CP31A using digestion-optimized RNA co-immunoprecipitation with deep sequencing (DO-RIP-seq). This identifies the mRNAs coding for subunits of the chloroplast NAD(P)H dehydrogenase (NDH) complex as main targets for CP31A. We demonstrate using whole-genome gene expression analysis and targeted RNA gel blot hybridization that the ndh mRNAs are all down-regulated in cp31a mutants. This diminishes the activity of the NDH complex. Our findings demonstrate how a chloroplast RNA binding protein can combine functionally related RNAs into one post-transcriptional operon. |
| Keywords: | Chloroplast, RNA Processing, RNA Binding, NDH Complex, RRM, Organelle, Arabidopsis thaliana |
| Source: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Publisher: | MDPI |
| Volume: | 21 |
| Number: | 16 |
| Page Range: | 5633 |
| Date: | 6 August 2020 |
| Official Publication: | https://doi.org/10.3390/ijms21165633 |
| PubMed: | View item in PubMed |
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