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| Item Type: | Article |
|---|---|
| Title: | CD4+ T cell mitochondrial genotype in Multiple Sclerosis: a cross-sectional and longitudinal analysis |
| Creators Name: | Cortes-Figueiredo, F. and Asseyer, S. and Chien, C. and Zimmermann, H.G. and Ruprecht, K. and Schmitz-Hübsch, T. and Bellmann-Strobl, J. and Paul, F. and Morais, V.A. |
| Abstract: | Multiple Sclerosis (MS) is a chronic autoimmune demyelinating disease of the central nervous system (CNS), with a largely unknown etiology, where mitochondrial dysfunction likely contributes to neuroaxonal loss and brain atrophy. Mirroring the CNS, peripheral immune cells from patients with MS, particularly CD4+ T cells, show inappropriate mitochondrial phenotypes and/or oxidative phosphorylation (OxPhos) insufficiency, with a still unknown contribution of mitochondrial DNA (mtDNA). We hypothesized that mitochondrial genotype in CD4+ T cells might influence MS disease activity and progression. Thus, we performed a retrospective cross-sectional and longitudinal study on patients with a recent diagnosis of either Clinically Isolated Syndrome (CIS) or Relapsing–Remitting MS (RRMS) at two timepoints: 6 months (VIS1) and 36 months (VIS2) after disease onset. Our primary outcomes were the differences in mtDNA extracted from CD4+ T cells between: (I) patients with CIS/RRMS (PwMS) at VIS1 and age- and sex-matched healthy controls (HC), in the cross-sectional analysis, and (II) different diagnostic evolutions in PwMS from VIS1 to VIS2, in the longitudinal analysis. We successfully performed mtDNA whole genome sequencing (mean coverage: 2055.77 reads/base pair) in 183 samples (61 triplets). Nonetheless, mitochondrial genotype was not associated with a diagnosis of CIS/RRMS, nor with longitudinal diagnostic evolution. |
| Keywords: | CD4-Positive T-Lymphocytes, Cross-Sectional Studies, Mitochondrial DNA, Demyelinating Diseases, Genotype, Longitudinal Studies, Multiple Sclerosis, Relapsing-Remitting Multiple Sclerosis, Retrospective Studies, T-Lymphocytes |
| Source: | Scientific Reports |
| ISSN: | 2045-2322 |
| Publisher: | Nature Publishing Group |
| Volume: | 14 |
| Number: | 1 |
| Page Range: | 7507 |
| Date: | 29 March 2024 |
| Official Publication: | https://doi.org/10.1038/s41598-024-57592-z |
| PubMed: | View item in PubMed |
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