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| Item Type: | Article |
|---|---|
| Title: | A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice |
| Creators Name: | Brenner, D. and Sieverding, K. and Srinidhi, J. and Zellner, S. and Secker, C. and Yilmaz, R. and Dyckow, J. and Amr, S. and Ponomarenko, A. and Tunaboylu, E. and Douahem, Y. and Schlag, J.S. and Rodríguez Martínez, L. and Kislinger, G. and Niemann, C. and Nalbach, K. and Ruf, W.P. and Uhl, J. and Hollenbeck, J. and Schirmer, L. and Catanese, A. and Lobsiger, C.S. and Danzer, K.M. and Yilmazer-Hanke, D. and Münch, C. and Koch, P. and Freischmidt, A. and Fetting, M. and Behrends, C. and Parlato, R. and Weishaupt, J.H. |
| Abstract: | Heterozygous mutations in the TBK1 gene can cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The majority of TBK1-ALS/FTD patients carry deleterious loss-of-expression mutations, and it is still unclear which TBK1 function leads to neurodegeneration. We investigated the impact of the pathogenic TBK1 missense variant p.E696K, which does not abolish protein expression, but leads to a selective loss of TBK1 binding to the autophagy adaptor protein and TBK1 substrate optineurin. Using organelle-specific proteomics, we found that in a knock-in mouse model and human iPSC-derived motor neurons, the p.E696K mutation causes presymptomatic onset of autophagolysosomal dysfunction in neurons precipitating the accumulation of damaged lysosomes. This is followed by a progressive, age-dependent motor neuron disease. Contrary to the phenotype of mice with full Tbk1 knock-out, RIPK/TNF-α-dependent hepatic, neuronal necroptosis, and overt autoinflammation were not detected. Our in vivo results indicate autophagolysosomal dysfunction as a trigger for neurodegeneration and a promising therapeutic target in TBK1-ALS/FTD. |
| Keywords: | Amyotrophic Lateral Sclerosis, Frontotemporal Dementia, Motor Neurons, Mutation, Neuroinflammatory Diseases, Phosphorylation, Protein Serine-Threonine Kinases, Animals, Mice |
| Source: | Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Volume: | 221 |
| Number: | 5 |
| Page Range: | e20221190 |
| Date: | 22 March 2024 |
| Official Publication: | https://doi.org/10.1084/jem.20221190 |
| PubMed: | View item in PubMed |
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