Chromatin accessibility dynamics across C. elegans development and ageing

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Item Type:

Article

Title:

Chromatin accessibility dynamics across C. elegans development and ageing

Creators Name:

Jänes, J. and Dong, Y. and Schoof, M. and Serizay, J. and Appert, A. and Cerrato, C. and Woodbury, C. and Chen, R. and Gemma, C. and Huang, N. and Kissiov, D. and Stempor, P. and Steward, A. and Zeiser, E. and Sauer, S. and Ahringer, J.

Abstract:

An essential step for understanding the transcriptional circuits that control development and physiology is the global identification and characterization of regulatory elements. Here we present the first map of regulatory elements across the development and ageing of an animal, identifying 42,245 elements accessible in at least one C. elegans stage. Based on nuclear transcription profiles, we define 15,714 protein-coding promoters and 19,231 putative enhancers, and find that both types of element can drive orientation-independent transcription. Additionally, more than 1000 promoters produce transcripts antisense to protein coding genes, suggesting involvement in a widespread regulatory mechanism. We find that the accessibility of most elements changes during development and/or ageing and that patterns of accessibility change are linked to specific developmental or physiological processes. The map and characterization of regulatory elements across C. elegans life provides a platform for understanding how transcription controls development and ageing.

Keywords:

ATAC-seq, Chromatin Accessibility, Chromosomes, Enhancer, Gene Expression, Genetics, Genomics, Promoter, Regulatory Element, Animals, Caenorhabditis elegans

Source:

eLife

ISSN:

2050-084X

Publisher:

eLife Sciences Publications

Volume:

Page Range:

e37344

Date:

26 October 2018

Official Publication: