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SNX27 and SORLA interact to reduce amyloidogenic subcellular distribution and processing of amyloid precursor protein

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Item Type:Article
Title:SNX27 and SORLA interact to reduce amyloidogenic subcellular distribution and processing of amyloid precursor protein
Creators Name:Huang, T.Y., Zhao, Y., Li, X., Wang, X., Tseng, I.C., Thompson, R., Tu, S., Willnow, T.E., Zhang, Y.W. and Xu, H.
Abstract:Proteolytic generation of amyloidogenic amyloid {beta} (A{beta}) fragments from the amyloid precursor protein (APP) significantly contributes to Alzheimer's disease (AD). Although amyloidogenic APP proteolysis can be affected by trafficking through genetically associated AD components such as SORLA, how SORLA functionally interacts with other trafficking components is yet unclear. Here, we report that SNX27, an endosomal trafficking/recycling factor and a negative regulator of the {gamma}-secretase complex, binds to the SORLA cytosolic tail to form a ternary complex with APP. SNX27 enhances cell surface SORLA and APP levels in human cell lines and mouse primary neurons, and depletion of SNX27 or SORLA reduces APP endosome-to-cell surface recycling kinetics. SNX27 overexpression enhances the generation of cell surface APP cleavage products such as soluble alpha-APP C-terminal fragment (CTF{alpha}) in a SORLA-dependent manner. SORLA-mediated A{beta} reduction is attenuated by downregulation of SNX27. This indicates that an SNX27/SORLA complex functionally interacts to limit APP distribution to amyloidogenic compartments, forming a non-amyloidogenic shunt to promote APP recycling to the cell surface.
Keywords:Alzheimer's Disease, APP, Endosome Recycling, Protein Trafficking, SNX27, SORLA, Animals, Mice
Source:Journal of Neuroscience
ISSN:0270-6474
Publisher:Society for Neuroscience
Volume:36
Number:30
Page Range:7996-8011
Date:27 July 2016
Official Publication:https://doi.org/10.1523/JNEUROSCI.0206-16.2016
PubMed:View item in PubMed

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