Browse by Journal Title

2018

Targeting the intrinsically disordered proteome using small-molecule ligands.
Wójcik, S. and Birol, M. and Rhoades, E. and Miranker, A.D. and Levine, Z.A.
Methods in Enzymology 611 : 703-734. 2018

2014

Pulsed stable isotope-resolved metabolomic studies of cancer cells.
Pietzke, M. and Kempa, S.
Methods in Enzymology 543 : 179-198. 9 June 2014

PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.
Spitzer, J. and Hafner, M. and Landthaler, M. and Ascano, M. and Farazi, T. and Wardle, G. and Nusbaum, J. and Khorshid, M. and Burger, L. and Zavolan, M. and Tuschl, T.
Methods in Enzymology 539 : 113-161. 25 February 2014

2013

Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway(R) recombination cloning technology and Flp-In T-REx(R) lines.
Spitzer, J. and Landthaler, M. and Tuschl, T.
Methods in Enzymology 529 : 99-124. 2013

2010

Gene knockdown in the mouse through RNAi.
Kleinhammer, A. and Wurst, W. and Kühn, R.
Methods in Enzymology 477 : 387-414. 2010

The power of reversibility regulating gene activities via tetracycline-controlled transcription.
Schoenig, K. and Bujard, H. and Gossen, M.
Methods in Enzymology 477 (C): 429-453. 2010

2005

Liposome-mediated suicide gene therapy in humans.
Reszka, R. and Jacobs, A. and Voges, J.
Methods in Enzymology 391 : 200-208. 1 January 2005

2003

Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy.
Phair, R.D. and Gorski, S.A. and Misteli, T.
Methods in Enzymology 375 : 393-414. 2003

2001

Scaffold protein regulation of mitogen-activated protein kinase cascade.
Catling, A.D. and Eblen, S.T. and Schaeffer, H.J. and Weber, M.J.
Methods in Enzymology 332 : 368-387. 1 January 2001

2000

Molecular parameters from sedimentation velocity experiments: whole boundary fitting using approximate and numerical solutions of lamm equation.
Demeler, B. and Behlke, J. and Ristau, O.
Methods in Enzymology 321 : 38-66. 1 January 2000

Analysis of RNA-protein cross-link sites by matrix-assisted laser desorption/ionization mass spectrometry and N-terminal microsequencing.
Thiede, B. and Wittmann-Liebold, B.
Methods in Enzymology 318 : 438-446. 1 January 2000

1999

Detection of polyglutamine aggregation in mouse models.
Davies, S.W. and Sathasivam, K. and Hobbs, C. and Doherty, P. and Mangiarini, L. and Scherzinger, E. and Wanker, E.E. and Bates, G.P.
Methods in Enzymology 309 : 687-701. 1 January 1999

Membrane filter assay for detection of amyloid-like polyglutamine-containing protein aggregates.
Wanker, E.E. and Scherzinger, E. and Heiser, V. and Sittler, A. and Eickhoff, H. and Lehrach, H.
Methods in Enzymology 309 : 375-386. 1 January 1999

1998

Purification and characterization of small heat shock proteins.
Buchner, J. and Ehrnsperger, M. and Gaestel, M. and Walke, S.
Methods in Enzymology 290 : 339-349. 1 January 1998

Molecular chaperones and their interactions investigated by analytical unltracentrifugation and other methodologies.
Schoenfeld, H.J. and Behlke, J.
Methods in Enzymology 290 : 269-296. 1 January 1998

1997

Use of tetracycline-controlled gene expression systems to study mammalian cell cycle.
Freundlieb, S. and Baron, U. and Bonin, A.L. and Gossen, M. and Bujard, H.
Methods in Enzymology 283 : 159-173. 1 January 1997

1996

Applying motif and profile searches.
Bork, P. and Gibson, T.
Methods in Enzymology 266 : 162-184. 1 January 1996

Generation of the cytosolic domain of microsomal P450 52A3 after high-level expression in Saccharomyces cerevisiae.
Scheller, U. and Juretzek, T. and Schunck, W.H.
Methods in Enzymology 272 : 65-75. 1 January 1996

1995

Avian hematopoietic cell culture: in vitro model systems to study oncogenic transformation of hematopoietic cells.
Beug, H. and Bartunek, P. and Steinlein, P. and Hayman, M.
Methods in Enzymology 254 : 41-76. 1 January 1995

Tightly regulated and inducible expression of dominant interfering dynamin mutant in stably transformed HeLa cells.
Damke, H. and Gossen, M. and Freundlieb, S. and Bujard, H. and Schmid, S.L.
Methods in Enzymology 257 : 209-220. 1995

1993

Polyethylene glycol and electric field-mediated cell fusion for formation of hybridomas.
Karsten, U. and Stolley, P. and Seidel, B.
Methods in Enzymology 220 : 228-238. 1 January 1993

1991

Purification, biological assay, and immunoassay of mammary- derived growth inhibitor.
Grosse, R. and Boehmer, F.D. and Langen, P. and Kurtz, A. and Lehmann, W. and Mieth, M. and Wallukat, G.
Methods in Enzymology 198 : 425-440. 1 January 1991

Identification of receptor-activated G proteins with photoreactive GTP analog, [alpha-32P]GTP azidoanilide.
Offermanns, S. and Schultz, G. and Rosenthal, W.
Methods in Enzymology 195 : 286-301. 1991

1990

Cell culture of bovine corneal endothelial cells and its application to transport studies.
Wiederholt, M. and Jentsch, T.J.
Methods in Enzymology 192 : 571-582. 1990

1985

Purification of Ns and Ni, the coupling proteins of hormone-sensitive adenylyl cyclases without intervention of activating regulatory ligands.
Codina, J. and Rosenthal, W. and Hildebrandt, J.D. and Birnbaumer, L. and Sekura, R.D.
Methods in Enzymology 109 : 446-465. 1985