Browse by Journal Title

2024

Quantification of microglial engulfment of synaptic material using flow cytometry.
Ugursu, B. and Wolf, S.A.
Journal of Visualized Experiments (207) 31 May 2024

Generation and downstream analysis of single-cell and single-nuclei transcriptomes in brain organoids.
Wandres, M., Aigner, D., Kastelic, N., Boltengagen, A., Rybak-Wolf, A. and Rajewsky, N.
Journal of Visualized Experiments (205): e66225. 29 March 2024

2022

Simultaneous imaging and flow-cytometry-based detection of multiple fluorescent senescence markers in therapy-induced senescent cancer cells.
Dovjak, E., Mairhofer, M., Wöß, C., Qi, J., Fan, D.N.Y., Schmitt, C.A. and Yu, Y.
Journal of Visualized Experiments (185): e63973. 12 July 2022

2021

Dual-color fluorescence cross-correlation spectroscopy to study protein-protein interaction and protein dynamics in live cells.
Hemmen, K., Choudhury, S., Friedrich, M., Balkenhol, J., Knote, F., Lohse, M.J. and Heinze, K.G.
Journal of Visualized Experiments 178 : e62954. 11 December 2021

Automated two-dimensional spatiotemporal analysis of mobile single-molecule FRET probes.
Schrangl, L., Göhring, J., Kellner, F., Huppa, J.B. and Schütz, G.J.
Journal of Visualized Experiments 177 : e63124. 23 November 2021

Automated two-dimensional spatiotemporal analysis of mobile single-molecule FRET probes.
Schrangl, L., Göhring, J., Kellner, F., Huppa, J.B. and Schütz, G.J.
[Video] 23 November 2021

Nuclei isolation from adult mouse kidney for single-nucleus RNA-sequencing.
Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N. and Schmidt-Ott, K.M.
Journal of Visualized Experiments (175): e62901. September 2021

Generation of human brain organoids for mitochondrial disease modeling.
Le, S., Petersilie, L., Inak, G., Menacho-Pando, C., Kafitz, K.W., Rybak-Wolf, A., Rajewsky, N., Rose, C.R. and Prigione, A.
Journal of Visualized Experiments (172): e62756. 21 June 2021

Isolation, culture, and genetic engineering of mammalian primary pigment epithelial cells for non-viral gene therapy.
Bascuas, T., Kropp, M., Harmening, N., Wong, B.M., Johnen, S., Izsvák, Z. and Thumann, G.
Journal of Visualized Experiments (168): e62145. 26 February 2021

Magnetic resonance imaging of multiple sclerosis at 7.0 Tesla.
Waiczies, S., Els, A., Kuchling, J., Markenroth Bloch, K., Pankowska, A., Waiczies, H., Herrmann, C., Chien, C., Finke, C., Paul, F. and Niendorf, T.
Journal of Visualized Experiments (168): e62142. 19 February 2021

Electroporation-based genetic modification of primary human pigment epithelial cells using the Sleeping Beauty transposon system.
Johnen, S., Harmening, N., Marie, C., Scherman, D., Izsvák, Z., Ivics, Z., Walter, P. and Thumann, G.
Journal of Visualized Experiments (168): e61987. February 2021

2020

Induction and analysis of oxidative stress in Sleeping Beauty transposon-transfected human retinal pigment epithelial cells.
Bascuas, T., Kropp, M., Harmening, N., Asrih, M., Izsvák, Z. and Thumann, G.
Journal of Visualized Experiments 2020 (166): e61957. 11 December 2020

2019

Cardiac magnetic resonance imaging at 7 Tesla.
Stäb, D., Al Najjar, A., O'Brien, K., Strugnell, W., Richer, J., Rieger, J., Niendorf, T. and Barth, M.
Journal of Visualized Experiments (143): e55853. 6 January 2019

2018

Candidate gene testing in clinical cohort studies with multiplexed genotyping and mass spectrometry.
Ashley, S.E., Meyer, B.A., Ellis, J.A. and Martino, D.J.
Journal of Visualized Experiments (136): e57601. 21 June 2018

Application of RNAi and heat-shock-induced transcription factor expression to reprogram germ cells to neurons in C. elegans.
Kolundzic, E., Seelk, S. and Tursun, B.
Journal of Visualized Experiments (131): e56889. 1 January 2018

2017

Chromatin immunoprecipitation (ChIP) protocol for low-abundance embryonic samples.
Rehimi, R., Bartusel, M., Solinas, F., Altmüller, J. and Rada-Iglesias, A.
Journal of Visualized Experiments (126): e56186. 29 August 2017

2016

Measurement of vibration detection threshold and tactile spatial acuity in human subjects.
Moshourab, R., Frenzel, H., Lechner, S., Haseleu, J., Bégay, V., Omerbašić, D. and Lewin, G.R.
Journal of Visualized Experiments (115): e52966. 1 September 2016

Using light sheet fluorescence microscopy to image zebrafish eye development.
Icha, J., Schmied, C., Sidhaye, J., Tomancak, P., Preibisch, S. and Norden, C.
Journal of Visualized Experiments 2016 (110): e53966. 10 April 2016

2015

Single molecule fluorescence microscopy on planar supported bilayers.
Axmann, M., Schütz, G.J. and Huppa, J.B.
Journal of Visualized Experiments (105): e53158. 31 October 2015

Measuring TCR-pMHC binding in situ using a FRET-based microscopy assay.
Axmann, M., Schütz, G.J. and Huppa, J.B.
Journal of Visualized Experiments (105): e53157. 30 October 2015

Automated quantification of hematopoietic cell - stromal cell interactions in histological images of undecalcified bone.
Zehentmeier, S., Cseresnyes, Z., Escribano Navarro, J., Niesner, R.A. and Hauser, A.E.
Journal of Visualized Experiments (98) 8 April 2015

Large-scale zebrafish embryonic heart dissection for transcriptional analysis.
Lombardo, V.A., Otten, C. and Abdelilah-Seyfried, S.
Journal of Visualized Experiments (95) 12 January 2015

2014

Highly resolved intravital striped-illumination microscopy of germinal centers.
Cseresnyes, Z., Oehme, L., Andresen, V., Sporbert, A., Hauser, A.E. and Niesner, R.
Journal of Visualized Experiments (86): e51135. 9 April 2014

2013

Culturing primary rat inner medullary collecting duct cells.
Faust, D., Geelhaar, A., Eisermann, B., Eichhorst, J., Wiesner, B., Rosenthal, W. and Klussmann, E.
Journal of Visualized Experiments (76): e50366. 21 June 2013

Monitoring dendritic cell migration using (19)F/(1)H magnetic resonance imaging.
Waiczies, H., Guenther, M., Skodowski, J., Lepore, S., Pohlmann, A., Niendorf, T. and Waiczies, S.
Journal of Visualized Experiments (73): e50251. 20 March 2013

Isolation and kv channel recordings in murine atrial and ventricular cardiomyocytes.
Koehncke, C., Lisewski, U., Schleussner, L., Gaertner, C., Reichert, S. and Roepke, T.K.
Journal of Visualized Experiments (73): e50145. 12 March 2013

Laser-inflicted injury of zebrafish embryonic skeletal muscle.
Otten, C. and Abdelilah-Seyfried, S.
Journal of Visualized Experiments 30 (71): e4351. 30 January 2013

2012

Cell tracking using photoconvertible proteins during zebrafish development.
Lombardo, V.A., Sporbert, A. and Abdelilah-Seyfried, S.
Journal of Visualized Experiments (67): e4350. 28 September 2012

A system for ex vivo culturing of embryonic pancreas.
Petzold, K.M. and Spagnoli, F.M.
Journal of Visualized Experiments (66): e3979. 27 August 2012

2011

DiI-labeling of DRG neurons to study axonal branching in a whole mount preparation of mouse embryonic spinal cord.
Schmidt, H. and Rathjen, F.G.
Journal of Visualized Experiments (58): e3667. 13 December 2011

2010

PAR-CliP: a method to identify transcriptome-wide the binding sites of RNA binding proteins.
Hafner, M., Landthaler, M., Burger, L., Khorshid, M., Hausser, J., Berninger, P., Rothballer, A., Ascano, M., Jungkamp, A.C., Munschauer, M., Ulrich, A., Wardle, G.S., Dewell, S., Zavolan, M. and Tuschl, T.
Journal of Visualized Experiments 41 (2034): e2034. 2 July 2010