Analysis of CD97 expression and manipulation: Antibody treatment but not gene targeting curtails granulocyte migration

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Item Type:	Article
Title:	Analysis of CD97 expression and manipulation: Antibody treatment but not gene targeting curtails granulocyte migration
Creators Name:	Veninga, H., Becker, S., Hoek, R.M., Wobus, M., Wandel, E., van der Kaa, J., van der Valk, M., de Vos, A.F., Haase, H., Owens, B., van der Poll, T., van Lier, R.A., Verbeek, J.S., Aust, G. and Hamann, J.
Abstract:	The heptahelical receptor CD97 is a defining member of the EGF-TM7 family of adhesion class receptors. In both humans and mice, CD97 isoforms are expressed with variable numbers of tandemly arranged N-terminal epidermal growth factor-like domains that facilitate interactions with distinct cellular ligands. Results from treatment of mice with mAbs in various disease models have suggested a role for CD97 in leukocyte trafficking. Here, we aimed to thoroughly characterize the expression profile of CD97, and delineate its biological function. To this end, we applied a novel polyclonal Ab, which is the first antiserum suitable for immunohistochemistry, and combined this analysis with the study of Cd97-lacZ knock-in mice. We show that similar to the situation in humans, hematopoietic, epithelial, endothelial, muscle, and fat cells expressed CD97. Despite this broad expression pattern, the Cd97(-/-) mouse that we created had no overt phenotype, except for a mild granulocytosis. Furthermore, granulocyte accumulation at sites of inflammation was normal in the absence of CD97. Interestingly, application of CD97 mAbs blocked granulocyte trafficking after thioglycollate-induced peritonitis in wild-type but not in knock-out mice. Hence, we conclude that CD97 mAbs actively induce an inhibitory effect that disturbs normal granulocyte trafficking, which is not perturbed by the absence of the molecule.
Keywords:	Antibodies, Cell Migration Inhibition, Gene Expression Regulation, Gene Targeting, Granulocytes, Immunophenotyping, Inflammation Mediators, Leukocytosis, Lung, Membrane Glycoproteins, Organ Specificity, Spleen, Animals, Mice
Source:	Journal of Immunology
ISSN:	0022-1767
Publisher:	American Association of Immunologists
Volume:	181
Number:	9
Page Range:	6574-6583
Date:	1 November 2008
Official Publication:	http://www.jimmunol.org/cgi/content/abstract/181/9/6574
PubMed:	View item in PubMed

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