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| Item Type: | Article |
|---|---|
| Title: | Phosphorylation of the L-type calcium channel beta subunit is involved in beta-adrenergic signal transduction in canine myocardium |
| Creators Name: | Haase, H., Karczewski, P., Beckert, R. and Krause, E.G. |
| Abstract: | Cyclic AMP-mediated phosphorylation of calcium channel subunits was studied in vitro and in vivo in preparations from dog heart. Calcium channels in native cardiac membranes were phosphorylated by cAMP-dependent protein kinase (PKA) solubilized with digitonin and subsequently immunoprecipitated using a polyclonal antibody generated against the deduced carboxy-terminal sequence of the cardiac beta subunit. A 62 kDa protein was identified as the major PKA-substrate in the immunoprecipitates. In the intact myocardium, this putative beta subunit was found to be phosphorylated in response to cAMP elevating agents. In contrast, no phosphorylation of a protein with an electrophoretic mobility similar to the alpha 1 subunit was detected, although 1,4-dihydropyridine receptor sites were recovered in the immunoprecipitates. Thus, we suggest that PKA-mediated phosphorylation of the beta subunit is the major mechanism for beta-adrenergic regulation of cardiac L-type calcium channel activity. |
| Keywords: | Calcium Channel, Canine Heart, Immunoprecipitation, Peptide Antibody, Protein Phosphorylation, Beta Subunit, Animals, Dogs |
| Source: | FEBS Letters |
| ISSN: | 0014-5793 |
| Publisher: | Elsevier |
| Volume: | 335 |
| Number: | 2 |
| Page Range: | 217-222 |
| Date: | 6 December 1993 |
| Official Publication: | https://doi.org/10.1016/0014-5793(93)80733-B |
| PubMed: | View item in PubMed |
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