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| Item Type: | Article |
|---|---|
| Title: | Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1 |
| Creators Name: | Dietrich, A., Kalwa, H., Storch, U., Mederos Y Schnitzler, M., Salanova, B., Pinkenburg, O., Dubrovska, G., Essin, K., Gollasch, M., Birnbaumer, L. and Gudermann, T. |
| Abstract: | Among the classical transient receptor potential (TRPC) subfamily, TRPC1 is described as a mechanosensitive and store-operated channel proposed to be activated by hypoosmotic cell swelling and positive pipette pressure as well as regulated by the filling status of intracellular Ca(2+) stores. However, evidence for a physiological role of TRPC1 may most compellingly be obtained by the analysis of a TRPC1-deficient mouse model. Therefore, we have developed and analyzed TRPC1(-/-) mice. Pressure-induced constriction of cerebral arteries was not impaired in TRPC1(-/-) mice. Smooth muscle cells from cerebral arteries activated by hypoosmotic swelling and positive pipette pressure showed no significant differences in cation currents compared to wild-type cells. Moreover, smooth muscle cells of TRPC1(-/-) mice isolated from thoracic aortas and cerebral arteries showed no change in store-operated cation influx induced by thapsigargin, inositol-1,4,5 trisphosphate, and cyclopiazonic acid compared to cells from wild-type mice. In contrast to these results, small interference RNAs decreasing the expression of stromal interaction molecule 1 (STIM1) inhibited thapsigargin-induced store-operated cation influx, demonstrating that STIM1 and TRPC1 are mutually independent. These findings also imply that, as opposed to current concepts, TRPC1 is not an obligatory component of store-operated and stretch-activated ion channel complexes in vascular smooth muscle cells. |
| Keywords: | TRPC1-Deficient Mice, Classical Transient Receptor Channel 1, Store-Operated Cation Influx, Pressure-Induced Cation Influx, Bayliss Effect, STIM1, Thapsigargin, Inositol-1,4,5 Trisphosphate, Animals, Mice |
| Source: | Pfluegers Archiv European Journal of Physiology |
| ISSN: | 0031-6768 |
| Publisher: | Springer |
| Volume: | 455 |
| Number: | 3 |
| Page Range: | 465-477 |
| Date: | December 2007 |
| Official Publication: | https://doi.org/10.1007/s00424-007-0314-3 |
| PubMed: | View item in PubMed |
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