![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
![[thumbnail of 911oa.pdf]](https://edoc.mdc-berlin.de/style/images/fileicons/application_pdf.png) |
PDF
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
6MB |
| Item Type: | Article |
|---|---|
| Title: | Tissue restricted expression and chromosomal localization of the YB-1 gene encoding a 42 kD nuclear CCAAT binding protein |
| Creators Name: | Spitkovsky, D.D., Royer-Pokora, B., Delius, H., Kisseljov, F., Jenkins, N.A., Gilbert, D.J., Copeland, N.G. and Royer, H.D. |
| Abstract: | YB-1 cDNA clones were isolated by binding site screening of a Hela expression library using a human papillomavirus type 18 enhancer oligonucleotide. YB-1 belongs to a family of transcription factors which bind to recognition sequences containing a core CCAAT element. YB-1 bound to its single stranded recognition sequence on the sense strand but not to the anti-sense strand. A synthetic peptide antiserum derived from the predicted YB-1 amino acid sequence identified a 42 kD nuclear protein in immunoblots. A protein with the same size was detected by binding site blotting experiments using the HPV18 enhancer oligonucleotide which bound YB-1. YB-1 gene expression was restricted in tissues from a human 24 week old fetus. High levels of YB-1 mRNA were present in heart, muscle, liver, lung, adrenal gland and the brain, in contrast, low amounts of YB-1 mRNA were found in thymus, kidney, bone marrow and spleen. In pancreas, bladder, stomach and testis YB-1 mRNA could not be detected by Northern hybridization. Finally, we have identified four YB-1 related loci in the mouse genome and have mapped these loci to four different mouse chromosomes by interspecific backcross analysis. |
| Keywords: | Amino Acid Sequence, Base Sequence, Binding Sites, Northern Blotting, CCAAT-Enhancer-Binding Proteins, Cultured Cells, Chromosome Mapping, HPV DNA Probes, DNA-Binding Proteins, Genetic Enhancer Elements, Gene Expression, Molecular Sequence Data, Multigene Family, NFI Transcription Factors, Nuclear Proteins, Transcription Factors, Y-Box-Binding Protein 1, Animals, Mice |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 20 |
| Number: | 4 |
| Page Range: | 797-803 |
| Date: | 25 February 1992 |
| Official Publication: | https://doi.org/10.1093/nar/20.4.797 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
