Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin

Tools

Item Type:	Article
Title:	Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin
Creators Name:	Jeannet, G., Scheller, M., Scarpellino, L., Duboux, S., Gardiol, N., Back, J., Kuttler, F., Malanchi, I., Birchmeier, W., Leutz, A., Huelsken, J. and Held, W.
Abstract:	The canonical Wnt signaling pathway plays key roles in stem cell maintenance, progenitor cell expansion and lineage decisions. Transcriptional responses induced by Wnt depend on the association of either beta-catenin or gamma-catenin with TCF/LEF transcription factors. Here we show that hematopoiesis, including thymopoiesis, is normal in the combined absence of beta- and gamma-catenin. Double-deficient hematopoietic stem cells (HSCs) maintain long-term repopulation capacity and multi lineage differentiation potential. Unexpectedly, two independent ex vivo reporter gene assays show that Wnt signal transmission is maintained in double-deficient HSCs, thymocytes, or peripheral T cells. In contrast, Wnt signaling is strongly reduced in thymocytes lacking TCF-1 or in non-hematopoietic cells devoid of beta-catenin. These data provide the first evidence that hematopoietic cells can transduce canonical Wnt signals in the combined absence of beta- and gamma-catenin.
Keywords:	Bone Marrow Cells, Cell Lineage, Hematopoiesis, Hematopoietic Stem Cells, Hybridomas, Signal Transduction, Spleen, T-Lymphocytes, Thymus Gland, Wnt Proteins, beta Catenin, gamma Catenin, Animals, Mice
Source:	Blood
ISSN:	0006-4971
Publisher:	American Society of Hematology
Volume:	111
Number:	1
Page Range:	142-149
Date:	1 January 2008
Official Publication:	https://doi.org/10.1182/blood-2007-07-102558
PubMed:	View item in PubMed

Repository Staff Only: item control page