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| Item Type: | Article |
|---|---|
| Title: | Sequence specificity of single-stranded DNA-binding proteins: a novel DNA microarray approach |
| Creators Name: | Morgan, H.P., Estibeiro, P., Wear, M.A., Max, K.E., Heinemann, U., Cubeddu, L., Gallagher, M.P., Sadler, P.J. and Walkinshaw, M.D. |
| Abstract: | We have developed a novel DNA microarray-based approach for identification of the sequence-specificity of single-stranded nucleic-acid-binding proteins (SNABPs). For verification, we have shown that the major cold shock protein (CspB) from Bacillus subtilis binds with high affinity to pyrimidine-rich sequences, with a binding preference for the consensus sequence, 5'-GTCTTTG/T-3'. The sequence was modelled onto the known structure of CspB and a cytosine-binding pocket was identified, which explains the strong preference for a cytosine base at position 3. This microarray method offers a rapid high-throughput approach for determining the specificity and strength of ss DNA-protein interactions. Further screening of this newly emerging family of transcription factors will help provide an insight into their cellular function. |
| Keywords: | Archaeal Proteins, Bacterial Proteins, Base Sequence, Binding Sites, Competitive Binding, Calorimetry, Carbocyanines, Consensus Sequence, Cytosine, Single-Stranded DNA, DNA-Binding Proteins, Electrophoretic Mobility Shift Assay, Fluorescent Dyes, Heat-Shock Proteins, Molecular Models, Oligonucleotide Array Sequence Analysis |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 35 |
| Number: | 10 |
| Page Range: | e75 |
| Date: | May 2007 |
| Official Publication: | https://doi.org/10.1093/nar/gkm040 |
| PubMed: | View item in PubMed |
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