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Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes

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Item Type:Article
Title:Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes
Creators Name:Doss, M.X., Winkler, J., Chen, S., Hippler-Altenburg, R., Sotiriadou, I., Halbach, M., Pfannkuche, K., Liang, H., Schulz, H., Hummel, O., Huebner, N., Rottscheidt, R., Hescheler, J. and Sachinidis, A.
Abstract:BACKGROUND: Characterisation of gene expression signatures for cardiomyocytes derived from embryonic stem (ES) cells will help define their early biological processes. RESULTS: A transgenic a-myosin heavy chain (a-MHC) ES cell lineage was generated, expressing puromycin resistance and enhanced green fluorescent protein (EGFP) under the control of the a-MHC promoter. A puromycin-resistant, EGFP+, a-MHC+ cardiomyocyte population was isolated with over 92% purity. RNA was isolated after electrophysiological characterisation of the cardiomyocytes. Comprehensive transcriptome analysis of a-MHC+ cardiomyocytes in comparison to undifferentiated a-MHC ES cells and the control population from 15-day old embryoid bodies (EBs) led to the identification of 884 upregulated probe sets and 951 downregulated probe sets in a-MHC+ cardiomyocytes. A subset of upregulated genes encodes for cytoskeletal and voltage-dependent channel proteins, and proteins participating in aerobic energy metabolism. Interestingly, mitosis, apoptosis and Wnt signalling-associated genes were downregulated in the cardiomyocytes. In contrast, annotations for genes upregulated in the a-MHC+ cardiomyocytes are enriched for the gene ontology categories: enzyme-linked receptor protein signalling pathway (GO:0007167); protein kinase activity (GO:0004672); negative regulation of Wnt receptor signalling pathway (GO:0030178); regulation of cell size (O:0008361); as well as for the Biocarta p38 MAPK signalling pathway and KEGG calcium signalling pathway. CONCLUSION: The specific pattern of gene expression in the cardiomyocytes derived from ES cells reflects the biological, physiological and functional processes occurring in mature cardiomyocytes. Identification of cardiomyocyte-specific gene expression patterns and signalling pathways will contribute towards elucidating their roles during intact cardiac function.
Keywords:Apoptosis, Cell Differentiation, Cell Proliferation, Cluster Analysis, Embryonic Stem Cells, Gene Expression Profiling, Gene Expression Regulation, Genetic Engineering, Cardiac Myocytes, Oligonucleotide Array Sequence Analysis, Messenger RNA, Ventricular Myosins, Animals, Mice
Source:Genome Biology
Publisher:BioMed Central
Page Range:R56
Date:11 April 2007
Official Publication:https://doi.org/10.1186/gb-2007-8-4-r56
PubMed:View item in PubMed

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