Targeting and tracing antigens in live cells with fluorescent nanobodies

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Item Type:	Article
Title:	Targeting and tracing antigens in live cells with fluorescent nanobodies
Creators Name:	Rothbauer, U., Zolghadr, K., Tillib, S., Nowak, D., Schermelleh, L., Gahl, A., Backmann, N., Conrath, K., Muyldermans, S., Cardoso, M.C. and Leonhardt, H.
Abstract:	We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
Keywords:	Antibodies, Antigen-Antibody Reactions, Antigens, Camels, Epitopes, Green Fluorescent Proteins, Immunoglobulin Heavy Chains, Mitosis, S Phase, Animals
Source:	Nature Methods
ISSN:	1548-7091
Publisher:	Nature Publishing Group
Volume:	3
Number:	11
Page Range:	887-889
Date:	November 2006
Official Publication:	https://doi.org/10.1038/nmeth953
PubMed:	View item in PubMed

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