Sec61p Is adjacent to nascent type-I and type-II signal- anchor proteins during their membrane insertion

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Item Type:	Article
Title:	Sec61p Is adjacent to nascent type-I and type-II signal- anchor proteins during their membrane insertion
Creators Name:	High, S., Andersen, S.S.L., Goerlich, D., Hartmann, E., Prehn, S., Rapoport, T.A. and Dobberstein, B.
Abstract:	We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. Goerlich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (Goerlich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that Sec61p is a core component of these sites.
Keywords:	Base Sequence, Biological Transport, DNA, Endoplasmic Reticulum, Glycosylphosphatidylinositols, Intracellular Membranes, Membrane Proteins, Molecular Sequence Data, Precipitin Tests
Source:	Journal of Cell Biology
ISSN:	0021-9525
Publisher:	Rockefeller University Press
Volume:	121
Number:	4
Page Range:	743-750
Date:	May 1993
Official Publication:	https://doi.org/10.1083/jcb.121.4.743
PubMed:	View item in PubMed

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