Designer T cells by T cell receptor replacement

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Item Type:	Article
Title:	Designer T cells by T cell receptor replacement
Creators Name:	Sommermeyer, D., Neudorfer, J., Weinhold, M., Leisegang, M., Engels, B., Noessner, E., Heemskerk, M.H., Charo, J., Schendel, D.J., Blankenstein, T., Bernhard, H. and Uckert, W.
Abstract:	T cell receptor (TCR) gene transfer is a convenient method to produce antigen-specific T cells for adoptive therapy. However, the expression of two TCR in T cells could impair their function or cause unwanted effects by mixed TCR heterodimers. With five different TCR and four different T cells, either mouse or human, we show that some TCR are strong - in terms of cell surface expression - and replace weak TCR on the cell surface, resulting in exchange of antigen specificity. Two strong TCR are co-expressed. A mouse TCR replaces human TCR on human T cells. Even though it is still poorly understood why some TCRalpha/beta combinations are preferentially expressed on T cells, our data suggest that, in the future, designer T cells with exclusive tumor reactivity can be generated by T cell engineering.
Keywords:	Gene Therapy, T cells, TCR, TCR Replacement, Animals, Mice
Source:	European Journal of Immunology
ISSN:	0014-2980
Publisher:	Wiley
Volume:	36
Number:	11
Page Range:	3052-3059
Date:	November 2006
Official Publication:	https://doi.org/10.1002/eji.200636539
PubMed:	View item in PubMed

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