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The translocation motif of hepatitis B virus improves protein vaccination

Item Type:Article
Title:The translocation motif of hepatitis B virus improves protein vaccination
Creators Name:Bleifuss, E., Kammertoens, T., Hutloff, A., Quarcoo, D., Dorner, M., Straub, P., Uckert, W. and Hildt, E.
Abstract:Cell-penetrating peptides (CPPs) have been shown to improve antigen loading of dendritic cell vaccines. Here we asked whether fusion of a CPP to a protein improves its immunogenicity when this fusion protein is directly applied as vaccine. We used the cell-penetrating translocation motif (TLM) derived from the hepatitis B virus, because no size limitation of cargos has been observed. Increased immunogenicity was observed when TLM was fused to ovalbumin (TLM-ova). TLM-ova was found to be superior to ova in inducing proliferation and cytotoxicity of ova-specific CD8+ T cells in vitro and in vivo. Using ovalbumin-expressing thymoma cells (EG7-ova), an improved anti-tumor immune response was observed for TLM-ova vaccination versus vaccination with ova. Moreover, TLM-ova vaccination induced a higher titer of anti-ovalbumin IgG2a antibodies compared to ova. These data demonstrate that CPP-protein vaccines can improve cellular as well as humoral immune responses.
Keywords:Cell penetrating peptide, Translocation motif of hepatitis B virus, Tumor immunity, Ovalbumin, Protein vaccination
Source:Cellular and Molecular Life Sciences
Page Range:627-635
Date:March 2006
Official Publication:https://doi.org/10.1007/s00018-005-5548-7
PubMed:View item in PubMed

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