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| Item Type: | Article |
|---|---|
| Title: | The translocation motif of hepatitis B virus improves protein vaccination |
| Creators Name: | Bleifuss, E., Kammertoens, T., Hutloff, A., Quarcoo, D., Dorner, M., Straub, P., Uckert, W. and Hildt, E. |
| Abstract: | Cell-penetrating peptides (CPPs) have been shown to improve antigen loading of dendritic cell vaccines. Here we asked whether fusion of a CPP to a protein improves its immunogenicity when this fusion protein is directly applied as vaccine. We used the cell-penetrating translocation motif (TLM) derived from the hepatitis B virus, because no size limitation of cargos has been observed. Increased immunogenicity was observed when TLM was fused to ovalbumin (TLM-ova). TLM-ova was found to be superior to ova in inducing proliferation and cytotoxicity of ova-specific CD8+ T cells in vitro and in vivo. Using ovalbumin-expressing thymoma cells (EG7-ova), an improved anti-tumor immune response was observed for TLM-ova vaccination versus vaccination with ova. Moreover, TLM-ova vaccination induced a higher titer of anti-ovalbumin IgG2a antibodies compared to ova. These data demonstrate that CPP-protein vaccines can improve cellular as well as humoral immune responses. |
| Keywords: | Cell penetrating peptide, Translocation motif of hepatitis B virus, Tumor immunity, Ovalbumin, Protein vaccination |
| Source: | Cellular and Molecular Life Sciences |
| ISSN: | 1420-682X |
| Volume: | 63 |
| Page Range: | 627-635 |
| Date: | March 2006 |
| Official Publication: | https://doi.org/10.1007/s00018-005-5548-7 |
| PubMed: | View item in PubMed |
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