Low stability of huntington muscle mitochondria against Ca2+ in R6/2 mice

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Item Type:	Article
Title:	Low stability of huntington muscle mitochondria against Ca2+ in R6/2 mice
Creators Name:	Gizatullina, Z.Z., Lindenberg, K.S., Harjes, P., Chen, Y., Kosinski, C.M., Landwehrmeyer, B.G., Ludolph, A.C., Striggow, F., Zierz, S. and Gellerich, F.N.
Abstract:	Objective: The aim of the present work was the detection of Mitochondrial dysfunction of Huntingdon's disease (HD). Methods: We investigated muscle and muscle mitochondria of 14- to 16-week-old R6/2 mice in comparison with wild-type mice. Results: Atrophic fibers, increased fuchsinophilic aggregates, and reduced cytochrome c oxidase (15%) were found in HD muscle. With swelling measurements and Ca 2+ accumulation experiments, a decreased stability of HD mitochondria against Ca 2+-induced permeability transition was detected. Complex I-dependent respiration of HD mitochondria was more sensitive to inhibition by adding 10μm Ca 2+ than wild-type mitochondria. Interpretation: Data suggest that the decreased stability of HD mitochondria against Ca 2+ contributes to energetic depression and cell atrophy.
Keywords:	Animal Disease Models, Calcium, Drug Dose-Response Relationship, Huntington Disease, Inbred C57BL Mice, Muscle Mitochondria, Nerve Tissue Proteins, Nuclear Proteins, Oxygen Consumption, Respiration, Time Factors, Transgenic Mice, Trinucleotide Repeats, Animals, Mice
Source:	Annals of Neurology
ISSN:	0364-5134
Publisher:	Wiley
Volume:	59
Number:	2
Page Range:	407-411
Date:	February 2006
Official Publication:	https://doi.org/10.1002/ana.20754
PubMed:	View item in PubMed

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