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Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system

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Item Type:Article
Title:Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system
Creators Name:Taxis, C., Keller, P., Kavagiou, Z., Jensen, L.J., Colombelli, J., Bork, P., Stelzer, E.H. and Knop, M.
Abstract:Spindle pole bodies (SPBs) provide a structural basis for genome inheritance and spore formation during meiosis in yeast. Upon carbon source limitation during sporulation, the number of haploid spores formed per cell is reduced. We show that precise spore number control (SNC) fulfills two functions. SNC maximizes the production of spores (1-4) that are formed by a single cell. This is regulated by the concentration of three structural meiotic SPB components, which is dependent on available amounts of carbon source. Using experiments and computer simulation, we show that the molecular mechanism relies on a self-organizing system, which is able to generate particular patterns (different numbers of spores) in dependency on one single stimulus (gradually increasing amounts of SPB constituents). We also show that SNC enhances intratetrad mating, whereby maximal amounts of germinated spores are able to return to a diploid lifestyle without intermediary mitotic division. This is beneficial for the immediate fitness of the population of postmeiotic cells.
Keywords:Carbon, Centromere, Computer Simulation, Cytoplasm, Fungal Gene Expression Regulation, Dominant Genes, Fungal Genes, Genotype, Green Fluorescent Proteins, Haploidy, Linkage, Meiosis, Electron Microscopy, Video Microscopy, Mitotic Spindle Apparatus, Biological Models, Mutation, Open Reading Frames, Plasmids, Genetic Recombination, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Software, Fungal Spores, Time Factors
Source:Journal of Cell Biology
Publisher:Rockefeller University Press
Page Range:627-640
Date:21 November 2005
Additional Information:Copyright (c) 2005 by Rockefeller University Press
Official Publication:https://doi.org/10.1083/jcb.200507168
PubMed:View item in PubMed

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