A human protein-protein interaction network: a resource for annotating the proteome

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Item Type:	Article
Title:	A human protein-protein interaction network: a resource for annotating the proteome
Creators Name:	Stelzl, U., Worm, U., Lalowski, M., Haenig, C., Brembeck, F.H., Goehler, H., Stroedicke, M., Zenkner, M., Schoenherr, A., Koeppen, S., Timm, J., Mintzlaff, S., Abraham, C., Bock, N., Kietzmann, S., Goedde, A., Toksoez, E., Droege, A., Krobitsch, S., Korn, B., Birchmeier, W., Lehrach, H. and Wanker, E.E.
Abstract:	Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and co-immunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes.
Keywords:	Databases as Topic, Intracellular Signaling Peptides and Proteins, Molecular Models, Nerve Tissue Proteins, Protein Binding, Proteins, Proteomics, Repressor Proteins, Two-Hybrid System Techniques
Source:	Cell
ISSN:	0092-8674
Publisher:	Cell Press
Volume:	122
Number:	6
Page Range:	957-968
Date:	23 September 2005
Additional Information:	The original publication is available at www.sciencedirect.com
Official Publication:	https://doi.org/10.1016/j.cell.2005.08.029
PubMed:	View item in PubMed

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