Elimination of human leukemia cells in NOD/SCID mice by WT1-TCR gene transduced human T cells

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Item Type:	Article
Title:	Elimination of human leukemia cells in NOD/SCID mice by WT1-TCR gene transduced human T cells
Creators Name:	Xue, S.A., Gao, L., Hart, D., Gillmore, R., Qasim, W., Thrasher, A., Apperley, J., Engels, B., Uckert, W., Morris, E. and Stauss, H.J.
Abstract:	Cytotoxic T lymphocytes (CTLs) specific for an HLA-A2-presented peptide epitope of the Wilms tumor antigen-1 (WT1) can selectively kill immature human leukemia progenitor and stem cells in vitro. In this study we have used retroviral gene transfer to introduce a WT1-specific T-cell receptor (TCR) into T lymphocytes obtained from patients with leukemia and from healthy donors. TCR-transduced T cells kill leukemia cells in vitro and display WT1-specific cytokine production. Intravenous injection of TCR-transduced T cells into nonobese diabetic-severe combined immunodeficiency (NOD/SCID) mice harboring human leukemia cells resulted in leukemia elimination, whereas transfer of control T cells transduced with an irrelevant TCR was ineffective. The data suggest that adoptive immunotherapy with WT1-TCR gene-modified patient T cells should be considered for the treatment of leukemia.
Keywords:	Antigen and T-Cell Receptors, Cell Line, Cell Proliferation, Health, Immunotherapy, Leukemia, T-Cell Receptor Genes, T-Lymphocytes, Transgenes, WT1 Proteins, Animals, Mice
Source:	Blood
ISSN:	0006-4971
Publisher:	American Society of Hematology
Volume:	106
Page Range:	3062-3067
Date:	14 July 2005
Official Publication:	https://doi.org/10.1182/blood-2005-01-0146
PubMed:	View item in PubMed

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