![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Article |
|---|---|
| Title: | Detection of cell-free DNA in bronchial lavage fluid supernatants of patients with lung cancer |
| Creators Name: | Carstensen, T., Schmidt, B., Engel, E., Jandrig, B., Witt, C. and Fleischhacker, M. |
| Abstract: | Recently, it was shown that it is possible to isolate free circulating DNA from plasma/serum of patients with benign and malignant diseases. In addition, several groups were able to detect tumor-associated alterations in these nucleic acids. We wondered whether any nucleic acids are detectable in cell-free bronchial lavage supernatants, which until now have been discarded after cell harvest. Additionally, we wanted to find out if it is possible to detect tumor-associated alterations in these DNA molecules. DNA was isolated from cell-free lavage supernatants from 30 lung cancer patients, and the DNA was examined for microsatellite alterations. Intact DNA could be isolated from all cell-free bronchial lavage supernatants. Microsatellite alterations were found in lavage supernatants of 12 of 30 patients and in lavage cells of 6 of 30 patients. Altogether, alterations were found in 14 of 30 patients. Thus, we could demonstrate for the first time that it is possible to isolate intact DNA from cell-free bronchial lavage supernatants. Their quantity and quality are sufficient for further amplification via polymerase chain reaction. Altogether, tumor-associated changes were detected in the DNA of 47% of the patients that were analyzed. |
| Keywords: | Bronchial Lavage, Free DNA, Lung Cancer, Method, Microsatellite Alteration, Polymerase Chain Reaction (PCR) |
| Source: | Annals of the New York Academy of Sciences |
| ISSN: | 0077-8923 |
| Publisher: | New York Academy of Sciences |
| Volume: | 1022 |
| Page Range: | 202-210 |
| Date: | 1 June 2004 |
| Official Publication: | https://doi.org/10.1196/annals.1318.031 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
