Detection of cell-free DNA in bronchial lavage fluid supernatants of patients with lung cancer

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Item Type:	Article
Title:	Detection of cell-free DNA in bronchial lavage fluid supernatants of patients with lung cancer
Creators Name:	Carstensen, T., Schmidt, B., Engel, E., Jandrig, B., Witt, C. and Fleischhacker, M.
Abstract:	Recently, it was shown that it is possible to isolate free circulating DNA from plasma/serum of patients with benign and malignant diseases. In addition, several groups were able to detect tumor-associated alterations in these nucleic acids. We wondered whether any nucleic acids are detectable in cell-free bronchial lavage supernatants, which until now have been discarded after cell harvest. Additionally, we wanted to find out if it is possible to detect tumor-associated alterations in these DNA molecules. DNA was isolated from cell-free lavage supernatants from 30 lung cancer patients, and the DNA was examined for microsatellite alterations. Intact DNA could be isolated from all cell-free bronchial lavage supernatants. Microsatellite alterations were found in lavage supernatants of 12 of 30 patients and in lavage cells of 6 of 30 patients. Altogether, alterations were found in 14 of 30 patients. Thus, we could demonstrate for the first time that it is possible to isolate intact DNA from cell-free bronchial lavage supernatants. Their quantity and quality are sufficient for further amplification via polymerase chain reaction. Altogether, tumor-associated changes were detected in the DNA of 47% of the patients that were analyzed.
Keywords:	Bronchial Lavage, Free DNA, Lung Cancer, Method, Microsatellite Alteration, Polymerase Chain Reaction (PCR)
Source:	Annals of the New York Academy of Sciences
ISSN:	0077-8923
Publisher:	New York Academy of Sciences
Volume:	1022
Page Range:	202-210
Date:	1 June 2004
Official Publication:	https://doi.org/10.1196/annals.1318.031
PubMed:	View item in PubMed

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