Transplantation of human acute myeloid leukemia (AML) cells in immunodeficient mice reveals altered cell surface phenotypes and expression of human endothelial markers

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Item Type:	Article
Title:	Transplantation of human acute myeloid leukemia (AML) cells in immunodeficient mice reveals altered cell surface phenotypes and expression of human endothelial markers
Creators Name:	Henschler, R., Goettig, S., Junghahn, I., Bug, G., Seifried, E., Mueller, A.M. and Fichtner, I.
Abstract:	To better characterize acute myeloid leukemia (AML) development in non-obese diabetic (NOD)/severe combined immunodeficiency (SCID) mice, we transplanted samples from patients with AML or KG-1 and EOL-1 cell lines. We found 9/12 primary AML samples and both cell lines to engraft within 2-8 weeks, with 5-80% human cells in bone marrow. Compared with freshly isolated AML cells, percentages of human CD33+, CD38+, CD31+ CD13+ or CD15+ subpopulations increased after transplantation, whereas percentages of CD34+ cells decreased. Engrafted mice frequently showed expression of human endothelial cell markers. Thus, transplantation of human AML into NOD/SCID mice reveals expression of hematopoietic and endothelial differentiation markers.
Keywords:	Acute myeloid leukemia, Bone marrow culture, Differentiation, Endothelial markers, NOD/SCID mice, Transplantation, Animals, Mice
Source:	Leukemia Research
ISSN:	0145-2126
Publisher:	Pergamon-Elsevier Science Ltd
Volume:	29
Number:	10
Page Range:	1191-1199
Date:	1 January 2005
Official Publication:	https://doi.org/10.1016/j.leukres.2005.03.019
PubMed:	View item in PubMed

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